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  2. Membrane Transporter/Ion Channel
  3. Monocarboxylate Transporter
  4. 7ACC1

7ACC1  (Synonyms: 香豆素D1421; DEAC; Coumarin D 1421; D 1421)

目錄號: HY-D0067 純度: 99.71%
COA 產(chǎn)品使用指南 技術支持

7ACC1 (DEAC; Coumarin D 1421; D 1421) 是一種單羧酸轉運蛋白 1 (MCT-1)/MCT-4 的特異性阻斷劑。7ACC1 能夠減弱腎癌細胞的增殖、遷移、侵襲，并下調(diào) MCT1/MCT4 表達水平和細胞外乳酸的含量。7ACC1 有望用于癌癥的研究。

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7ACC1 Chemical Structure

7ACC1 Chemical Structure

CAS No. : 50995-74-9

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥178
In-stock
250 mg ￥162
In-stock
1 g ￥228
In-stock
5 g   詢價  
10 g   詢價  

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Customer Review

    7ACC1 purchased from MCE. Usage Cited in: Cancer Med. 2018 Sep;7(9):4690-4700.  [Abstract]

    The protein expression of MCT4 in the murine breast cancer cell line 4T1 treated with 7ACC1 (0.1 mM) or transfected with different ShMCT4 vectors.

    查看 Monocarboxylate Transporter 亞型特異性產(chǎn)品:

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    7ACC1 (DEAC; Coumarin D 1421; D 1421) is a monocarboxylate transporter 1 (MCT-1)/MCT-4 specifc blocker. 7ACC1 attenuates renal cancer cell proliferation, migration, invasion and down-regulates the levels of MCT1/MCT4 expression and extracellular lactate. 7ACC1 is promising for research of cancers[1][2][3].

    IC50 & Target

    MCT1

     

    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    4T1 IC50
    > 100 μM
    Compound: 7
    Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell proliferation by MTT assay
    Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell proliferation by MTT assay
    		[PMID: 34626786]
    HepG2 IC50
    > 100 μM
    Compound: 10
    Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 28666736]
    MCF-10A IC50
    > 100 μM
    Compound: 7
    Cytotoxicity against human MCF-10A cells assessed as inhibition of cell proliferation by MTT assay
    Cytotoxicity against human MCF-10A cells assessed as inhibition of cell proliferation by MTT assay
    		[PMID: 34626786]
    MCF7 IC50
    > 100 μM
    Compound: 10
    Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
    Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay
    		[PMID: 28666736]
    MDA-MB-231 IC50
    > 100 μM
    Compound: 10
    Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay
    Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay
    		[PMID: 28666736]
    MDA-MB-231 IC50
    > 100 μM
    Compound: 7
    Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation by MTT assay
    Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation by MTT assay
    		[PMID: 34626786]
    SiHa IC50
    0.86 μM
    Compound: 17
    Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
    Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
    		[PMID: 24095010]
    SiHa EC50
    1.8 μM
    Compound: 17
    Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
    Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
    		[PMID: 24095010]
    SiHa IC50
    250 nM
    Compound: 17
    Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting
    Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 12 mins by liquid scintillation counting
    		[PMID: 24095010]
    SK-HEP1 IC50
    > 100 μM
    Compound: 10
    Antiproliferative activity against human SKHEP1 cells after 48 hrs by MTT assay
    Antiproliferative activity against human SKHEP1 cells after 48 hrs by MTT assay
    		[PMID: 28666736]
    WiDr IC50
    > 100 μM
    Compound: 7
    Antiproliferative activity against human WiDr cells assessed as inhibition of cell proliferation by MTT assay
    Antiproliferative activity against human WiDr cells assessed as inhibition of cell proliferation by MTT assay
    		[PMID: 34626786]
    體外研究
    (In Vitro)

    7ACC1 (10 μM, 0-96 小時) 在 786-O 細胞和 HUVECs 的共培養(yǎng)條件下減弱了細胞的生存能力，降低了細胞遷移和侵襲能力[1]。
    7ACC1 (10 μM, 24 小時) 抑制了 786-O 細胞與 HUVECs 共培養(yǎng)條件下 MCT1 和 MCT4 的蛋白表達，降低了細胞外乳酸水平[1]。
    7ACC1 (0-1 μM, 24 小時) 抑制癌細胞內(nèi)乳酸的內(nèi)流，但不影響外流[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[1]

    Cell Line: 786-O cells and HUVECs
    Concentration: 10 μM
    Incubation Time: 24 h
    Result: Attenuated the protein expressions of MCT1 and MCT4 in co-culturing of 786-O cells and HUVECs.
    體內(nèi)研究
    (In Vivo)

    7ACC1 (3 mg/kg，腹腔注射，每日一次，持續(xù) 12 天) 能抑制在小鼠中表達 MCT 的宮頸 SiHa，結直腸 HCT116 和乳腺 MCF-7 腫瘤的生長[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: mice bearing tumors, including cervix cancer SiHa xenografts but also tumors derived from human colon cancer cells HCT116 and bladder cancer cells UM-UC3[2]
    Dosage: 3 mg/kg
    Administration: i.p., daily for 12 days
    Result: Led to significant SiHa and HCT116 tumor growth delays in mice bearing tumors.
    分子量

    261.27

    Formula

    C14H15NO4
    CAS 號
    性狀

    固體

    顏色

    Orange to red

    Emission (Em)

    445
    Excitation (Ex)

    345
    中文名稱

    香豆素D1421
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : 33.33 mg/mL (127.57 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 3.8275 mL 19.1373 mL 38.2746 mL
    5 mM 0.7655 mL 3.8275 mL 7.6549 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (9.57 mM); 懸濁液; 超聲助溶

      此方案可獲得 2.5 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.71%

    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.8275 mL 19.1373 mL 38.2746 mL 95.6865 mL
    5 mM 0.7655 mL 3.8275 mL 7.6549 mL 19.1373 mL
    10 mM 0.3827 mL 1.9137 mL 3.8275 mL 9.5686 mL
    15 mM 0.2552 mL 1.2758 mL 2.5516 mL 6.3791 mL
    20 mM 0.1914 mL 0.9569 mL 1.9137 mL 4.7843 mL
    25 mM 0.1531 mL 0.7655 mL 1.5310 mL 3.8275 mL
    30 mM 0.1276 mL 0.6379 mL 1.2758 mL 3.1895 mL
    40 mM 0.0957 mL 0.4784 mL 0.9569 mL 2.3922 mL
    50 mM 0.0765 mL 0.3827 mL 0.7655 mL 1.9137 mL
    60 mM 0.0638 mL 0.3190 mL 0.6379 mL 1.5948 mL
    80 mM 0.0478 mL 0.2392 mL 0.4784 mL 1.1961 mL
    100 mM 0.0383 mL 0.1914 mL 0.3827 mL 0.9569 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    7ACC1
    目錄號：
    HY-D0067
    需求量：