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  4. Calcein-AM

Calcein-AM  (Synonyms: 鈣黃綠素-AM; Calcein acetoxymethyl ester)

目錄號: HY-D0041 純度: 96.00%
COA 產(chǎn)品使用指南

Calcein AM，中文名為鈣黃綠素乙酰氧基甲酯，具細(xì)胞膜滲透性，可輕易進(jìn)入到細(xì)胞內(nèi)，Calcein AM 身并無熒光，進(jìn)入細(xì)胞后被細(xì)胞中內(nèi)源性酯酶水解生成具有強負(fù)電荷的不能通透細(xì)胞膜的極性分子鈣黃綠素 (Calcein)，從而被滯留在細(xì)胞內(nèi)。而 Calcein 可發(fā)出強綠色熒光，因此常與 Propidium Iodide 用于細(xì)胞活力/毒力檢測，激發(fā)/發(fā)射波長：494/515 nm。

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Calcein-AM Chemical Structure

Calcein-AM Chemical Structure

CAS No. : 148504-34-1

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規(guī)格 價格 是否有貨 數(shù)量
1 mg ￥2760
In-stock
5 mg   詢價  
10 mg   詢價  

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Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 50 篇科研文獻(xiàn)

IF

    Calcein-AM purchased from MCE. Usage Cited in: Bioact Mater. 2022 Aug 11;21:20-31.  [Abstract]

    HUVECs proliferation is assessed by Calcein-AM, and the results show that HP-β-CD and HP-β-CD@Res could effectively promote the proliferation of HUVECs.

    Calcein-AM purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(30):e2203031.  [Abstract]

    XPS analysis of CaALN powder before and after immersing in FeCl3 solution for 24 h. Detection of intracellular Fe concentration using Calcein-AM in MGC 803 cells after treatment with NaALN, CaALN, and DFX at 25 mg/L for 24 h by FCM analysis. Then these cells are washed with PBS and incubated with Calcein-AM (0.5 μM) for 15 min at 37 °C in the dark.

    Calcein-AM purchased from MCE. Usage Cited in: Pharmaceuticals. 2022 Aug 26;15(9):1059.

    For U251 cells, Calcein-AM (10 μM) solution and 0.5 μL PI staining solution (15 μM) is added into each well, stained for 10 min.

    Calcein-AM purchased from MCE. Usage Cited in: Antioxidants (Basel). 2021 May 18;10(5):798.  [Abstract]

    HeLa cells are pretreated with 20 μM Fe(II) for 2 h and then incubated with Calcein-AM ( 0.5 μM; for 15 min at 37 °C). After 15 min, cells are washed and incubated with or without CAPE and its analogues for 15 min. The fluorescence intensity of cells is measured by flow cytometry.

    Calcein-AM purchased from MCE. Usage Cited in: Biochem Pharmacol. 2021 Jun;188:114583.  [Abstract]

    Cells are incubated with fluorescence probes Calcein-AM (1 μM) and propidium iodide (5 μM) for 5 min and imaged through fluorescence inverted microscope.

    Calcein-AM purchased from MCE. Usage Cited in: Acta Pharm Sin B. 2020 Sep;10(9):1694-1708.  [Abstract]

    HUVECs are pre-treated with SMI or Rd for 24 h and seeded on Matrigel for 5.5 h followed by 0.5 h of Calcein-AM staining.

    Calcein-AM purchased from MCE. Usage Cited in: EMBO Rep. 2019 Jul;20(7):e47563.  [Abstract]

    Cells are trypsinized, washed twice with 0.5 ml PBS, and then incubated with 0.2 μM Calcein‐AM at 37°C for 15 min. Then, the cells were washed twice with 0.5 ml PBS and either incubated with 100 μM Deferiprone or left untreated at 37°C for 1 h. The cells are analyzed with a flow cytometer. Calcein-AM is excited at 488 nm, and fluorescence is measured at 525 nm.

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Calcein AM, has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein), which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm[1].

    體外研究
    (In Vitro)

    使用方法
    1.Calcein AM 工作液的配制
    用 DMSO 配置 5 mM 儲備液，再用預(yù)熱好的無血清細(xì)胞培養(yǎng)基或 PBS 稀釋儲存液，配制成 1-10 μM 的 Calcein AM 工作液。
    注：請根據(jù)實際情況調(diào)整 Calcein AM 工作液濃度，且現(xiàn)用現(xiàn)配。
    2. 細(xì)胞染色（懸浮細(xì)胞）
    2.1 離心收集細(xì)胞，加入 PBS 洗滌兩次，每次 5 分鐘。細(xì)胞密度在 1×106/mL。
    2.2 加入 1 mL Calcein AM 工作液，37℃ 孵育 30-45 分鐘。
    2.3 400 g，離心 3-4 分鐘，棄去上清。
    2.4 加入 PBS 洗滌細(xì)胞兩次，每次 5 分鐘。
    2.5 用 1 mL 無血清培養(yǎng)基或 PBS 重懸細(xì)胞后，使用熒光顯微鏡或流式細(xì)胞儀進(jìn)行觀察。
    3. 細(xì)胞染色（貼壁細(xì)胞）
    3.1 將貼壁細(xì)胞培養(yǎng)于無菌蓋玻片上。
    3.2 從培養(yǎng)基中移出蓋玻片，吸除多余培養(yǎng)基。
    3.3 加入 100 μL 染料工作液，輕輕晃動使其完全覆蓋細(xì)胞，37℃ 孵育 30-45 分鐘。
    3.4 吸去染料工作液，用培養(yǎng)基洗 2-3 次，每次 5 分鐘，使用熒光顯微鏡或熒光酶標(biāo)檢測儀進(jìn)行觀察。

    保存條件
    -20℃ 避光保存

    注意事項
    1. 請根據(jù)實際情況調(diào)整 Calcein AM 工作液濃度與孵育時間。
    2. 本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品。
    3. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    Calcein-AM 被發(fā)現(xiàn)適用于體內(nèi)研究，因為它對細(xì)胞功能沒有有害影響，而且確實是細(xì)胞活力的標(biāo)志物[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    994.86

    Formula

    C46H46N2O23
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    Emission (Em)

    515
    Excitation (Ex)

    485
    中文名稱

    鈣黃綠素-AM
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性數(shù)據(jù)
    細(xì)胞實驗： 

    DMSO 中的溶解度 : 100 mg/mL (100.52 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.0052 mL 5.0258 mL 10.0517 mL
    5 mM 0.2010 mL 1.0052 mL 2.0103 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    計算結(jié)果
    工作液所需濃度 : mg/mL
    純度 & 產(chǎn)品資料

    純度: ≥98.0%

    染色示例
    參考文獻(xiàn)
    Cell Assay
    [1][2][3]

    K562, Daudi, and Chang liver cells are labeled with calcein-AM. Calcein-AM's excitation and emission wavelengths are 496 nm and 520 nm, respectively. The filter/mirror combination used to detect calcein-AM's green fluorescence includes the 490-nm excitation and 520-nm emission filters with a dichroic mirror. Differences in the automatic fluorescence readings between the test and control wells determine the results[1]. A simple and sensitive cell-cell adhesion microplate assay is established using the calcein-AM. The procedure involves three steps: the labeling of lymphocytes with an adequate concentration of calcein-AM (20 μM) during a short incubation period (30 min); the adhesion of 2×105 labeled lymphocytes per well to confluent keratinocyte or fibroblast monolayers grown in microtiter plates for 90 min; and, finally, measurement of the fluorescent signal utilizing a new system of cold-light microfluorimetry[2]. Cells are incubated for 15 min in 1 mL of a 1% saponin solution in PBS buffer, pH 7.4, containing 0.05% sodium azide. After saponin permeabilization, 4×105 RBCs in suspension in PBS buffer containing 0.1% saponin and 0.05% sodium azide are incubated (37°C in the dark for 45 min) with calcein-AM to a final concentration of 5 μM, ished three times with the same PBS buffer containing 0.1% saponin and 0.05% sodium azide, and the cell viability is analyzed by flow cytometry[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.0052 mL 5.0258 mL 10.0517 mL 25.1292 mL
    5 mM 0.2010 mL 1.0052 mL 2.0103 mL 5.0258 mL
    10 mM 0.1005 mL 0.5026 mL 1.0052 mL 2.5129 mL
    15 mM 0.0670 mL 0.3351 mL 0.6701 mL 1.6753 mL
    20 mM 0.0503 mL 0.2513 mL 0.5026 mL 1.2565 mL
    25 mM 0.0402 mL 0.2010 mL 0.4021 mL 1.0052 mL
    30 mM 0.0335 mL 0.1675 mL 0.3351 mL 0.8376 mL
    40 mM 0.0251 mL 0.1256 mL 0.2513 mL 0.6282 mL
    50 mM 0.0201 mL 0.1005 mL 0.2010 mL 0.5026 mL
    60 mM 0.0168 mL 0.0838 mL 0.1675 mL 0.4188 mL
    80 mM 0.0126 mL 0.0628 mL 0.1256 mL 0.3141 mL
    100 mM 0.0101 mL 0.0503 mL 0.1005 mL 0.2513 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Calcein-AM
    目錄號：
    HY-D0041
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