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  1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Menadione

Menadione  (Synonyms: 維生素K3; Vitamin K3)

目錄號: HY-B0332 純度: 99.53%
COA 產(chǎn)品使用指南

Menadione 是一種萘醌,可在體內(nèi)轉(zhuǎn)化為活性維生素K2。Menadione 是一種潛在的抗癌劑和放射增敏劑。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報,我們不為任何個人用途提供產(chǎn)品和服務

Menadione Chemical Structure

Menadione Chemical Structure

CAS No. : 58-27-5

1.  客戶無需承擔相應的運輸費用。

2.  同一機構(gòu)(單位)同一產(chǎn)品試用裝僅限申領(lǐng)一次,同一機構(gòu)(單位)一年內(nèi)

     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ¥550
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500 mg ¥500
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1 g ¥650
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5 g ¥1500
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10 g ¥2000
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50 g   詢價  

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Customer Review

Other Forms of Menadione:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Menadione is a naphthoquinone that is converted into active vitamin K2 in the body. Menadione is a potential anticancer agent and radiosensitizer[1].

IC50 & Target

Human Endogenous Metabolite

 

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
A2058 IC50
13.4 μM
Compound: Menadione
Cytotoxicity against human A2058 cells assessed as inhibition of formazan formation at day 5 by measuring absorbance at 430 nm
Cytotoxicity against human A2058 cells assessed as inhibition of formazan formation at day 5 by measuring absorbance at 430 nm
[PMID: 19969400]
A2780 IC50
2.6 μM
Compound: 13
Antiproliferative activity against human A2780 cells after 2 days by alamar-blue assay
Antiproliferative activity against human A2780 cells after 2 days by alamar-blue assay
[PMID: 19028102]
A549 IC50
15.89 μM
Compound: VK3
Cytotoxicity in human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity in human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 35085721]
A549 IC50
25.1 μM
Compound: 18
Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
DU-145 IC50
11 μM
Compound: mnd
Antiproliferative activity against human androgen-independent prostate carcinoma DU145 cell line by colorimetric assay
Antiproliferative activity against human androgen-independent prostate carcinoma DU145 cell line by colorimetric assay
[PMID: 16216500]
DU-145 IC50
9.86 μM
Compound: Menadione
Cytotoxicity against human DU145 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human DU145 cells assessed as growth inhibition after 72 hrs by MTS assay
[PMID: 23791367]
GES1 IC50
16.85 μM
Compound: VK3
Toxicity in human GES1 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Toxicity in human GES1 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 35085721]
HaCaT IC50
15.8 μM
Compound: Menadione
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
Antihyperproliferative activity against human HaCaT cells after 48 hrs by MTT assay
[PMID: 28410493]
HaCaT IC50
15.8 μM
Compound: MD
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopic analysis
[PMID: 26840368]
HaCaT IC50
15.8 μM
Compound: Menadione
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells assessed as inhibition of cell growth after 48 hrs by phase contrast microscopy
[PMID: 24964246]
HaCaT IC50
15.8 μM
Compound: menadione
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
Antihyperproliferative activity against human HaCaT cells after 48 hrs by phase contrast microscopy
[PMID: 22845014]
HCT-116 IC50
12.37 μM
Compound: VK3
Cytotoxicity in human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity in human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 35085721]
HeLa IC50
18 μM
Compound: 1d
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 24913712]
HeLa IC50
24.7 μM
Compound: Menadione
Cytotoxicity against human HeLa cells assessed as inhibition of formazan formation at day 6 by measuring absorbance at 430 nm
Cytotoxicity against human HeLa cells assessed as inhibition of formazan formation at day 6 by measuring absorbance at 430 nm
[PMID: 19969400]
HFF-1 IC50
> 50 μM
Compound: 3
Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
Cytotoxicity against HFF-1 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
[PMID: 34438124]
HGC-27 IC50
9.7 μM
Compound: VK3
Cytotoxicity in human HGC-27 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity in human HGC-27 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 35085721]
HL-60 IC50
5.6 μM
Compound: 18
Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
HL-60 IC50
7.51 μM
Compound: VK3
Cytotoxicity in human HL-60 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity in human HL-60 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 35085721]
HT-29 IC50
13.6 μM
Compound: VK3
Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity in human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 35085721]
HT-29 IC50
9.69 μM
Compound: Menadione
Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by MTS assay
[PMID: 23791367]
Jurkat IC50
5 μM
Compound: 18
Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
LS174T IC50
26.6 μM
Compound: 18
Cytotoxicity against human LS174T cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human LS174T cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
MCF7 IC50
9 μM
Compound: 18
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
MDA-MB-231 IC50
12.68 μM
Compound: Menadione
Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTS assay
Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTS assay
[PMID: 23791367]
MGC-803 IC50
11.85 μM
Compound: VK3
Cytotoxicity in human MGC-803 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity in human MGC-803 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 35085721]
MIA PaCa-2 IC50
6.2 μM
Compound: mnd
Antiproliferative activity against human pancreatic carcinoma Mia PaCa2 cell line by colorimetric assay
Antiproliferative activity against human pancreatic carcinoma Mia PaCa2 cell line by colorimetric assay
[PMID: 16216500]
MONO-MAC-6 IC50
6.2 μM
Compound: 18
Cytotoxicity against human MONO-MAC-6 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human MONO-MAC-6 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
N2a IC50
2.688 μM
Compound: Menadione
Inhibition of reactive oxygen species production in mouse Neuro2a cells assessed as decrease in H2O2 levels after 6 hrs by ROS-Glo luminescence assay
Inhibition of reactive oxygen species production in mouse Neuro2a cells assessed as decrease in H2O2 levels after 6 hrs by ROS-Glo luminescence assay
[PMID: 28152427]
PBMC IC50
14.5 μM
Compound: 18
Cytotoxicity against human PBMC cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human PBMC cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
SAS IC50
14 μM
Compound: 1d
Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human SAS cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 24913712]
SW982 IC50
9.3 μM
Compound: 18
Cytotoxicity against human SW982 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human SW982 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
THP-1 IC50
6.3 μM
Compound: 18
Cytotoxicity against human THP1 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human THP1 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
U-937 IC50
6.3 μM
Compound: 18
Cytotoxicity against human U937 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
Cytotoxicity against human U937 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay
[PMID: 31563013]
Vero IC50
6.5 μg/mL
Compound: 21, menadione
Cytotoxicity against Vero cells after 48 hrs by XTT method
Cytotoxicity against Vero cells after 48 hrs by XTT method
[PMID: 17888665]
體外研究
(In Vitro)

Menadione (0-100 μM, 24-72 h) 可在高濃度 (100 μM) 下誘導 AR4-2J 細胞壞死,在低濃度 (10-20 μM) 下誘導細胞凋亡[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[2]

Cell Line: AR4-2J cells
Concentration: 0-100 μM
Incubation Time: 24-72 h
Result: Inhibited cell proliferation in time- and dose-dependently manner at lower concentrations (1-20 μM).

Western Blot Analysis[2]

Cell Line: AR4-2J cells
Concentration: 0-100 μM
Incubation Time: 24 h
Result: Induced wild-type P53 at the dose of 1-20 μM.
Induced a low level of wild-type P53 at the dose of 100 μM.
體內(nèi)研究
(In Vivo)

Menadione (25-150 mg/kg, 靜脈注射, 一次或每隔一天共五次) 在雄性 Wister 大鼠中單次注射比五次注射產(chǎn)生更嚴重的損傷[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wister rats[1]
Dosage: 25-150 mg/kg
Administration: i.v., once time or every other day for five times
Result: Showed microgranular degeneration of renal tubular cells at the dose of 25 mg/kg.
Observed microgranular degeneration of renal tubular cells and mild pulmonary hemorrhage at the dose of 50 mg/kg.
Produced lesions in the kidney, heart, liver and lung at the dose of 100-150 mg/kg.
Showed kidney apoptosis at the dose of 100-150 mg/kg.
Clinical Trial
分子量

172.18

Formula

C11H8O2

CAS 號
性狀

固體

顏色

Light yellow to green yellow

中文名稱

維生素K3-d3

結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

溶解性數(shù)據(jù)
細胞實驗: 

DMSO 中的溶解度 : 125 mg/mL (725.98 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響,請使用新開封的 DMSO)

Ethanol 中的溶解度 : 9.09 mg/mL (52.79 mM; 超聲助溶)

H2O 中的溶解度 : 0.1 mg/mL (0.58 mM; 超聲助溶)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 5.8079 mL 29.0394 mL 58.0788 mL
5 mM 1.1616 mL 5.8079 mL 11.6158 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)。-80°C儲存時,請在1年內(nèi)使用, -20°C儲存時,請在6個月內(nèi)使用。

* 備注:如您選擇水作為儲備液,請稀釋至工作液后,再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗:

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:
——為保證實驗結(jié)果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當保存;體內(nèi)實驗的工作液,建議您現(xiàn)用現(xiàn)配,當天使用;
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (12.08 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL(飽和度未知)的澄清溶液。

    1 mL 工作液為例,取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中,混合均勻;再向上述體系中加入 50 μL Tween-80,混合均勻;然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL

    生理鹽水的配制:將 0.9 g 氯化鈉,溶解于 ddH?O 并定容至 100 mL,可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (12.08 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL(飽和度未知)的澄清溶液。

    1 mL 工作液為例,取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中,混合均勻。

    2 g SBE-β-CD(磺丁基醚 β-環(huán)糊精)粉末定容于 10 mL 的生理鹽水中,完全溶解至澄清透明。
動物溶解方案計算器
請輸入動物實驗的基本信息:

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

由于實驗過程有損耗,建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠,建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括:DMSO, ,均可在 MCE 網(wǎng)站選購。 ,Tween 80,均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO(母液濃度為 mg/mL)。

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度,以下方案僅供參考,如有需要,請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液,加入 μL 。 μL ,混合均勻至澄清,再加 μL Tween 80,混合均勻至澄清,再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上,請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài),從左到右依次添加助溶劑。您可采用超聲加熱 (超聲清洗儀,建議頻次 20-40 kHz),渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.53%

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)。-80°C儲存時,請在1年內(nèi)使用, -20°C儲存時,請在6個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 5.8079 mL 29.0394 mL 58.0788 mL 145.1969 mL
5 mM 1.1616 mL 5.8079 mL 11.6158 mL 29.0394 mL
10 mM 0.5808 mL 2.9039 mL 5.8079 mL 14.5197 mL
15 mM 0.3872 mL 1.9360 mL 3.8719 mL 9.6798 mL
20 mM 0.2904 mL 1.4520 mL 2.9039 mL 7.2598 mL
25 mM 0.2323 mL 1.1616 mL 2.3232 mL 5.8079 mL
30 mM 0.1936 mL 0.9680 mL 1.9360 mL 4.8399 mL
40 mM 0.1452 mL 0.7260 mL 1.4520 mL 3.6299 mL
50 mM 0.1162 mL 0.5808 mL 1.1616 mL 2.9039 mL
DMSO 60 mM 0.0968 mL 0.4840 mL 0.9680 mL 2.4199 mL
80 mM 0.0726 mL 0.3630 mL 0.7260 mL 1.8150 mL
100 mM 0.0581 mL 0.2904 mL 0.5808 mL 1.4520 mL

* 備注:如您選擇水作為儲備液,請稀釋至工作液后,再用 0.22 μm 的濾膜過濾除菌后使用。

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱:
Menadione
目錄號:
HY-B0332
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