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  2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. NO Synthase Endogenous Metabolite
  4. L-NMMA acetate

L-NMMA acetate  (Synonyms: Tilarginine acetate; Methylarginine acetate)

目錄號(hào): HY-18732A 純度: ≥99.0%
COA 產(chǎn)品使用指南 技術(shù)支持

L-NMMA acetate是一氧化氮合酶 (nitric oxide synthase) 抑制劑, 抑制一氧化氮合酶的所有亞型NOS1，NOS2和NOS3。對(duì)nNOS (大鼠), eNOS (人), and iNOS (小鼠) 的Ki 值分別為0.18，0.4，6 μM。

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L-NMMA acetate Chemical Structure

L-NMMA acetate Chemical Structure

CAS No. : 53308-83-1

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規(guī)格 價(jià)格 是否有貨 數(shù)量
1 mg ￥361
In-stock
5 mg ￥950
In-stock
10 mg ￥1350
In-stock
25 mg ￥2363
In-stock
50 mg ￥3488
In-stock
100 mg   詢價(jià)  
200 mg   詢價(jià)  

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Customer Review

Other Forms of L-NMMA acetate:

    L-NMMA acetate purchased from MCE. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173.  [Abstract]

    L-NMMA acetate (200?μM; 48 h) leads to elevating ammonia and inhibiting CD8+ TM cell induction, in parallel with the inhibition of NO production.

    查看 NO Synthase 亞型特異性產(chǎn)品:

    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    L-NMMA acetate is a nitric oxide synthase inhibitor of all NOS isoforms including NOS1, NOS2, and NOS3. The Ki values for nNOS (rat), eNOS (human), and iNOS (mouse) are approximately 0.18, 0.4, and 6 μM, respectively.

    IC50 & Target

    eNOS

     

    iNOS

     

    nNOS

     

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    RAW264.7 IC50
    17 μM
    Compound: L-NMMA
    Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay
    Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pre-incubated for 2 hrs before LPS stimulation for 24 hrs by nitrate/nitrite assay
    		[PMID: 31095383]
    RAW264.7 IC50
    20.34 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay
    Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by Griess reagent based assay
    		[PMID: 35503996]
    RAW264.7 CC50
    214.41 μM
    Compound: L-NMMA
    Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay
    Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability measured after 12 hrs by MTT assay
    		[PMID: 35503996]
    RAW264.7 IC50
    39.9 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay
    Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs by Griess assay
    		[PMID: 28654256]
    RAW264.7 IC50
    47.2 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay
    Inhibition of LPS-induced NO production in mouse RAW264.7 cells incubated for 18 hrs by Griess reagent based assay
    		[PMID: 33325237]
    RAW264.7 IC50
    78.3 μM
    Compound: L-NMMA
    Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis
    Inhibition of NO production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent based analysis
    		[PMID: 34890996]
    體外研究
    (In Vitro)

    L-NMMA, starting from 100 μM, produces a concentration-dependent inhibition of the evoked relaxations (2Hz); maximal inhibition at 1 mM averaged about 35%. The inhibitory effect of L-NMMA is unchanged by previous incubation with D-arginine while it is prevented by L-arginine (L-Arg). L-NMMA does not affect isoprenaline-induced relaxation[2]. Superfusion of L-NMMA reduces arteriolar diameter and causes dose-dependent increases in arteriolar tone. The onset of action of L-NMMA is nearly immediate. L-NMMA inhibits vasodilator responses to the endothelium-dependent vasodilator ACh but not to the endothelium-independent NP. NE induced dose-related vasoconstriction that is significantly potentiated by L-NMMA[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    248.28

    Formula

    C9H20N4O4
    CAS 號(hào)
    性狀

    固體

    顏色

    White to off-white

    結(jié)構(gòu)分類(lèi)
    初始來(lái)源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    H2O 中的溶解度 : ≥ 50 mg/mL (201.39 mM)

    * "≥" means soluble, but saturation unknown.

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 4.0277 mL 20.1386 mL 40.2771 mL
    5 mM 0.8055 mL 4.0277 mL 8.0554 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑： PBS

      Solubility: 100 mg/mL (402.77 mM); 澄清溶液; 超聲助溶

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請(qǐng)具體參考實(shí)測(cè) 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: ≥99.0%

    參考文獻(xiàn)
    Kinase Assay
    [1]

    NOS activity is determined by monitoring the conversion of L-[14C]arginine to [14C]citrulline. For nNOS, reaction mixtures contain, in a final volume of 200 μL, 20 mM Na+ HEPES buffer, pH 7.5, 100 μM EDTA, 2 mM CaCl2, 10 μg/mL calmodulin, 500 μM dithiothreitol, 100 μM THB, 25 μM FAD, 25 μM FMN, 100 μg/mL bovine serum albumin, 20 μM L-[14C]citrulline, 500 PM NADPH, and enzyme. Reaction is initiated by the addition of L-[14C]citrulline; reaction temperature is 25°C. At appropriate times, typically 3.5, 7, and 10.5 min, 50-1.11 portions are removed and added to 200 pl of a stopping solution of 100 mM Na+ HEPES buffer, pH 5.5, containing 5 mM EGTA. Those samples are immediately heated for 1 min in a boiling water bath, chilled, and centrifuged. A portion (225 μL) of the supernatant is fractionated on small Dowex 50 columns. [14C]citrulline is eluted with 2 ml of water and is quantitated by liquid scintillation counting[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 4.0277 mL 20.1386 mL 40.2771 mL 100.6928 mL
    5 mM 0.8055 mL 4.0277 mL 8.0554 mL 20.1386 mL
    10 mM 0.4028 mL 2.0139 mL 4.0277 mL 10.0693 mL
    15 mM 0.2685 mL 1.3426 mL 2.6851 mL 6.7129 mL
    20 mM 0.2014 mL 1.0069 mL 2.0139 mL 5.0346 mL
    25 mM 0.1611 mL 0.8055 mL 1.6111 mL 4.0277 mL
    30 mM 0.1343 mL 0.6713 mL 1.3426 mL 3.3564 mL
    40 mM 0.1007 mL 0.5035 mL 1.0069 mL 2.5173 mL
    50 mM 0.0806 mL 0.4028 mL 0.8055 mL 2.0139 mL
    60 mM 0.0671 mL 0.3356 mL 0.6713 mL 1.6782 mL
    80 mM 0.0503 mL 0.2517 mL 0.5035 mL 1.2587 mL
    100 mM 0.0403 mL 0.2014 mL 0.4028 mL 1.0069 mL

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱(chēng)：
    L-NMMA acetate
    目錄號(hào)：
    HY-18732A
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