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  2. Anti-infection Metabolic Enzyme/Protease Apoptosis Membrane Transporter/Ion Channel Neuronal Signaling
  3. Bacterial Antibiotic HIF/HIF Prolyl-Hydroxylase Apoptosis MDM-2/p53 Potassium Channel Calcium Channel
  4. Minocycline hydrochloride

Minocycline hydrochloride  (Synonyms: 鹽酸米諾環(huán)素)

目錄號: HY-17412 純度: 99.79%
COA 產(chǎn)品使用指南

Minocycline hydrochloride 是一種口服有效、能透過血腦屏障的半合成四環(huán)素類抗生素。Minocycline hydrochloride 是一種缺氧誘導(dǎo)因子 (HIF-1α) 抑制劑。Minocycline hydrochloride 具有抗癌(anti-cancer)，抗炎(anti-inflammatory) 和谷氨酸 (glutamate) 拮抗作用。Minocycline hydrochloride 降低谷氨酸神經(jīng)傳遞，顯示神經(jīng)保護特性和抗抑郁作用。Minocycline hydrochloride 通過與細菌核糖體30S亞基結(jié)合，抑制細菌蛋白的合成，從而產(chǎn)生抑菌 (bacteriostatic) 作用。

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Minocycline hydrochloride Chemical Structure

Minocycline hydrochloride Chemical Structure

CAS No. : 13614-98-7

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥500
In-stock
50 mg ￥450
In-stock
100 mg ￥650
In-stock
200 mg   詢價  
500 mg   詢價  

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Customer Review

Other Forms of Minocycline hydrochloride:

MCE 顧客使用本產(chǎn)品發(fā)表的 36 篇科研文獻

Proliferation Assay
IF

    Minocycline hydrochloride purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Aug 30;15(1):245.  [Abstract]

    Immunostaining of the trigeminal nucleus caudalis (TNC) for Iba1 in the NTG group and the NTG+Minocycline (Mino) group on day 9.

    Minocycline hydrochloride purchased from MCE. Usage Cited in: Neurochem Res. 2017 Oct;42(10):2698-2711.  [Abstract]

    a Statistical analysis showing the prevention effect of minocycline pretreatment (40 mg/kg/day) on CUS-, CRS- or CSDS-induced decreases in hippocampal microglial numbers. b, c Statistical analysis showing the prevention effect of minocycline pretreatment (40 mg/kg/day) on CUS-, CRS- or CSDS-induced increases in the immobile time in the TST (b) and FST (c).

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Minocycline hydrochloride is an orally active, potent and BBB-penetrated semi-synthetic tetracycline antibiotic. Minocycline hydrochloride is a hypoxia-inducible factor (HIF)-1α inhibitor. Minocycline hydrochloride shows anti-cancer, anti-inflammatory, and glutamate antagonist effects. Minocycline hydrochloride reduces glutamate neurotransmission and shows neuroprotective properties and antidepressant effects. Minocycline hydrochloride inhibits bacterial protein synthesis through binding with the 30S subunit of the bacterial ribosome, resulting in a bacteriostatic effect[1][2][3][4][5][6][7].

    IC50 & Target

    L-type calcium channel

     

    體外研究
    (In Vitro)

    Minocycline hydrochloride (0-100 μM，24-72 小時) 抑制卵巢癌細胞系 (OVCAR-3、SKOV-3 和 A2780) 的增殖和克隆形成活性[3]。
    Minocycline hydrochloride (0-100 μM，24-48 h) 通過抑制細胞周期蛋白和抑制 DNA 摻入來抑制細胞周期[3]。
    Minocycline hydrochloride (0-100 μM，72 h)) 在卵巢癌細胞系中誘導(dǎo)細胞凋亡[3]。
    Minocycline hydrochloride 顯示出直接的神經(jīng)元保護作用，這種保護模式可能與線粒體完整性和細胞色素 c 的保護有關(guān)，隨后抑制 caspase 依賴性和非 caspase 依賴性細胞死亡[2]。
    Minocycline hydrochloride 導(dǎo)致缺氧誘導(dǎo)因子 (HIF)-1α 的抑制，并伴有 up-p53 蛋白水平的調(diào)節(jié)和 AKT/mTOR/p70S6K/4E-BP1 通路的失活[6]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Proliferation Assay[3]

    Cell Line: Human ovarian cancer cell lines (OVCAR-3, SKOV-3 and A2780) and primary cells (HEK-293, HMEC, HUVEC, ATCC)
    Concentration: 0, 1, 10, 50 and 100 μM
    Incubation Time: 24, 48 or 72 h
    Result: Inhibited proliferation of OVCAR-3, SKOV-3 and A2780 cells in a concentration-dependent manner, with IC50 values of 62.0, 56.1 and 59.5 μM, respectively. Had no effect on the viability of HEK-293 or HUVEC.

    Western Blot Analysis[3]

    Cell Line: OVCAR-3, SKOV-3 and A2780 cells
    Concentration: 0, 10, 50 and 100 μM
    Incubation Time: 72 h
    Result: Expressed lower levels of cyclins A, B and E. Increased caspase-3 levels by more than 3.0 fold in the 100 μM. Minocycline-activated caspase-3 in turn led to cleavage of PARP-1. Increased the degradation product p89 of PARP-1 by caspase-3.

    Cell Cycle Analysis[3]

    Cell Line: OVCAR-3, SKOV-3 and A2780 cells
    Concentration: 0, 10, 50 and 100 μM
    Incubation Time: 24 or 48 h
    Result: Arrested cells in the G0-G1 phase in a concentration and time-dependent manner. Declined percentage of cells in the S and G2-M phases in excess of 80% each at 100 μM.
    體內(nèi)研究
    (In Vivo)

    Minocycline hydrochloride (0-30 mg/kg，口服，每日一次，持續(xù) 4 周) 抑制雌性裸鼠 OVCAR-3 腫瘤的生長[3]。
    Minocycline hydrochloride (IP) 是一種有效的藥物當(dāng)腹腔內(nèi)給予高劑量時，腦缺血動物模型中的神經(jīng)保護劑[1]。
    Minocycline hydrochloride (0-40 mg/kg，IP，一次) 顯著減弱 METH 誘導(dǎo)的過度運動和小鼠行為敏化的發(fā)展[2]。
    Minocycline hydrochloride (3 和 10 mg/kg，IV，一次) 可有效減少臨時大腦中動脈閉塞模型中的梗塞面積 (TMCAO)[1]。
    Minocycline hydrochloride (3-10 mg/kg，IV，一次) 導(dǎo)致血清水平 (3 mg/kg) 與人類在標(biāo)準(zhǔn) 200 mg 劑量[1]。
    Minocycline hydrochloride 可減輕大鼠缺血引起的室性心律失常。這種作用可能與 PI3K/Akt 信號通路、線粒體 KATP 通道和 L 型 Ca2+ 通道的激活有關(guān)[7]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female nude mice (6 weeks old, 9 per group, OVCAR-3 cells were injected s.c. into the left flank of each mouse)[3]
    Dosage: 10 or 30 mg/kg
    Administration: Administered orally in the drinking water, initiated on day 8 of cell inoculation, daily for 4 weeks
    Result: Suppressed OVCAR-3 tumor growth in these female nude mice, and reduced microvessel density.
    Animal Model: Male Balb/cAnNCrICrIj mice (8 weeks old, 23-30 g, methamphetamine (METH, 3 mg/kg) was injected subcutaneously (s.c.) in a volume of 10 ml/kg)[2]
    Dosage: 0, 10, 20, or 40 mg/kg
    Administration: IP, once, 30 min before the administration of METH
    Result: Significantly attenuated METH-induced hyperlocomotion and the development of behavioral sensitization in mice at 40 mg/kg. Did not exert any effect on the induction of METH-induced hyperthermia in mice. Significantly attenuated the reduction of DA and DOPAC in the striatum. Significantly attenuated the reduction of DAT-immunoreactivity in the mouse striatum. Significantly attenuated the increase in MAC1-immunoreactivity in the striatum after the administration of METH.
    Animal Model: Male Sprague-Dawley rats (270-330 g, TMCAO model)[1]
    Dosage: 3 mg/kg and 10 mg/kg
    Administration: IV, once, 4, 5, or 6 hours post TMCAO
    Result: Reduced infarct size by 42% while 10 mg/kg reduced infarct size by 56% at doses of 3 mg/kg; significantly reduced infarct size at 5 hours by 40% at doses of 10 mg/kg and the 3 mg/kg dose significantly reduced infarct size by 34%. With a 6 hour time window there was a non-significant trend in infarct reduction.
    Animal Model: Male Sprague-Dawley rats (270-330 g)[1]
    Dosage: 3, 10, or 20 mg/kg
    Administration: IV, once
    Result: Peak concentrations of serum levels of minocycline averaged 3.6, 13.0 and 28.8 mg/L with 3, 10 and 20 mg/kg doses respectively. The serum levels of minocycline at a 3 mg/kg dose (3.6 mg/L) were similar to that reported in humans after a standard 200 mg dose. Did not significantly affect hemodynamic and physiological variables.
    Clinical Trial
    分子量

    493.94

    Formula

    C23H28ClN3O7
    CAS 號
    性狀

    固體

    顏色

    Light yellow to yellow

    中文名稱

    鹽酸米諾環(huán)素；二甲胺四環(huán)素；二甲胺四環(huán)素鹽酸鹽
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : 19.23 mg/mL (38.93 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    H2O 中的溶解度 : 9.09 mg/mL (18.40 mM; 超聲助溶)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.0245 mL 10.1227 mL 20.2454 mL
    5 mM 0.4049 mL 2.0245 mL 4.0491 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： PBS

      Solubility: 7.69 mg/mL (15.57 mM); 澄清溶液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    計算結(jié)果
    工作液所需濃度 : mg/mL
    純度 & 產(chǎn)品資料

    純度: 99.79%

    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.0245 mL 10.1227 mL 20.2454 mL 50.6134 mL
    5 mM 0.4049 mL 2.0245 mL 4.0491 mL 10.1227 mL
    10 mM 0.2025 mL 1.0123 mL 2.0245 mL 5.0613 mL
    15 mM 0.1350 mL 0.6748 mL 1.3497 mL 3.3742 mL
    DMSO 20 mM 0.1012 mL 0.5061 mL 1.0123 mL 2.5307 mL
    25 mM 0.0810 mL 0.4049 mL 0.8098 mL 2.0245 mL
    30 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6871 mL

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Minocycline hydrochloride
    目錄號：
    HY-17412
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