PFI-1

目錄號(hào): HY-16586 純度: 99.87%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南技術(shù)支持

PFI-1 是一種有效的 BET 抑制劑，能夠有效抑制 BRD4，IC₅₀ 值為 0.22 μM。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

PFI-1 Chemical Structure

CAS No. : 1403764-72-6

1. 客戶無(wú)需承擔(dān)相應(yīng)的運(yùn)輸費(fèi)用。

2. 同一機(jī)構(gòu)（單位）同一產(chǎn)品試用裝僅限申領(lǐng)一次，同一機(jī)構(gòu)（單位）一年內(nèi)

可免費(fèi)申領(lǐng)三個(gè)不同產(chǎn)品的試用裝。

3. 試用裝只面向終端客戶。

規(guī)格	價(jià)格	是否有貨
10 mM * 1 mL in DMSO	￥484	In-stock
1 mg	￥176	In-stock
5 mg	￥440	In-stock
10 mg	￥800	In-stock
50 mg	￥2200	In-stock
100 mg	￥4000	In-stock
200 mg		詢價(jià)
500 mg		詢價(jià)

or 大包裝詢價(jià)

* Please select Quantity before adding items.

Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻(xiàn)

•Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):2961-2966. [Abstract]
•Patent. US20180263995A1.

PFI-1 相關(guān)產(chǎn)品

•相關(guān)化合物庫(kù):

•同靶點(diǎn)產(chǎn)品:

•同靶點(diǎn)蛋白產(chǎn)品:

生物活性
純度 & 產(chǎn)品資料
參考文獻(xiàn)

生物活性

PFI-1 is a selective BET (bromodomain-containing protein) inhibitor for BRD4 with IC₅₀ of 0.22 μM in a cell-free assay.

IC₅₀ & Target

IC50: 0.22 μM (BRD4)

細(xì)胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
786-0	GI₅₀	12 μM Compound: 7; PFI-1	Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
A498	GI₅₀	1.8 μM Compound: 7; PFI-1	Growth inhibition of human A498 cells after 48 hrs by SRB assay Growth inhibition of human A498 cells after 48 hrs by SRB assay	[PMID: 29776834]
A549	GI₅₀	19.5 μM Compound: 7; PFI-1	Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
ACHN	GI₅₀	7.8 μM Compound: 7; PFI-1	Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
BT-549	GI₅₀	14.8 μM Compound: 7; PFI-1	Growth inhibition of human BT549 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human BT549 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
CAKI-1	GI₅₀	3.6 μM Compound: 7; PFI-1	Growth inhibition of human CAKI-1 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human CAKI-1 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
CCRF-CEM	GI₅₀	7.8 μM Compound: 7; PFI-1	Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
COLO 205	GI₅₀	3 μM Compound: 7; PFI-1	Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
DU-145	GI₅₀	28.8 μM Compound: 7; PFI-1	Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCC 2998	GI₅₀	9.1 μM Compound: 7; PFI-1	Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCC 2998	GI₅₀	93.3 μM Compound: 7; PFI-1	Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCT-116	GI₅₀	4.5 μM Compound: 7; PFI-1	Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HCT-15	GI₅₀	3.2 μM Compound: 7; PFI-1	Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HL-60	IC₅₀	2.19 μM Compound: 1; PFI-1	Cytotoxicity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 27266999]
HL-60	IC₅₀	3.99 μM Compound: PFI-1	Antiproliferative activity against human HL60 cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human HL60 cells incubated for 72 hrs by CCK8 assay	[PMID: 32883643]
HL-60(TB)	GI₅₀	3.4 μM Compound: 7; PFI-1	Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HOP-62	GI₅₀	2.5 μM Compound: 7; PFI-1	Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HOP-92	GI₅₀	1.2 μM Compound: 7; PFI-1	Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
Hs-578T	GI₅₀	1.9 μM Compound: 7; PFI-1	Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
HT-29	IC₅₀	14.72 μM Compound: PFI-1	Antiproliferative activity against human HT29 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human HT29 cells incubated for 72 hrs by MTT assay	[PMID: 32883643]
HT-29	GI₅₀	3 μM Compound: 7; PFI-1	Growth inhibition of human HT-29 cells after 48 hrs by SRB assay Growth inhibition of human HT-29 cells after 48 hrs by SRB assay	[PMID: 29776834]
IGROV-1	GI₅₀	6.3 μM Compound: 7; PFI-1	Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
K562	GI₅₀	4.6 μM Compound: 7; PFI-1	Growth inhibition of human K562 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human K562 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
KM12	GI₅₀	10.7 μM Compound: 7; PFI-1	Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
LOX IMVI	GI₅₀	3.5 μM Compound: 7; PFI-1	Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
M14	GI₅₀	2.9 μM Compound: 7; PFI-1	Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MCF7	GI₅₀	1.5 μM Compound: 7; PFI-1	Growth inhibition of human MCF7 cells after 48 hrs by SRB assay Growth inhibition of human MCF7 cells after 48 hrs by SRB assay	[PMID: 29776834]
MDA-MB-231	GI₅₀	3.5 μM Compound: 7; PFI-1	Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MDA-MB-435	GI₅₀	2.6 μM Compound: 7; PFI-1	Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MDA-MB-468	GI₅₀	1.5 μM Compound: 7; PFI-1	Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MOLT-4	GI₅₀	10.5 μM Compound: 7; PFI-1	Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
MV4-11	IC₅₀	7.75 μM Compound: 1; PFI-1	Cytotoxicity against human MV4-11 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human MV4-11 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 27266999]
NCI/ADR-RES	GI₅₀	17.4 μM Compound: 7; PFI-1	Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H226	GI₅₀	14.1 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H226 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H226 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H23	GI₅₀	8.5 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H23 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H23 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H322M	GI₅₀	6 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H322M cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H322M cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H460	GI₅₀	9.3 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H460 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H460 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
NCI-H522	GI₅₀	8.7 μM Compound: 7; PFI-1	Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-3	GI₅₀	4 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-4	GI₅₀	6.3 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-5	GI₅₀	10.5 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR5 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR5 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
OVCAR-8	GI₅₀	9.1 μM Compound: 7; PFI-1	Growth inhibition of human OVCAR8 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR8 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
PBMC	EC₅₀	1.89 μM Compound: 17, PFI-1, PF-6405761, 4E96	Inhibition of LPS-induced IL6 production in human PBMC by ELISA Inhibition of LPS-induced IL6 production in human PBMC by ELISA	[PMID: 23095041]
PC-3	GI₅₀	28.2 μM Compound: 7; PFI-1	Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
RPMI-8226	GI₅₀	2.2 μM Compound: 7; PFI-1	Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
RXF 393	GI₅₀	7.9 μM Compound: 7; PFI-1	Growth inhibition of human RXF393 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human RXF393 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SF-268	GI₅₀	28.8 μM Compound: 7; PFI-1	Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SF-295	GI₅₀	13.5 μM Compound: 7; PFI-1	Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SF-539	GI₅₀	4.5 μM Compound: 7; PFI-1	Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-MEL-2	GI₅₀	29.5 μM Compound: 7; PFI-1	Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-MEL-28	GI₅₀	6.2 μM Compound: 7; PFI-1	Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-MEL-5	GI₅₀	6.3 μM Compound: 7; PFI-1	Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SK-OV-3	GI₅₀	4.9 μM Compound: 7; PFI-1	Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SN12C	GI₅₀	6.8 μM Compound: 7; PFI-1	Growth inhibition of human SN12C cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SN12C cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SNB-19	GI₅₀	4.8 μM Compound: 7; PFI-1	Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SNB-75	GI₅₀	0.4 μM Compound: 7; PFI-1	Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SR	GI₅₀	3.7 μM Compound: 7; PFI-1	Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
SW-620	GI₅₀	12 μM Compound: 7; PFI-1	Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
T47D	GI₅₀	3.7 μM Compound: 7; PFI-1	Growth inhibition of human T47D cells after 48 hrs by sulforhodamine B assay Growth inhibition of human T47D cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
THP-1	IC₅₀	0.43 μM Compound: 1; PFI-1	Antiinflammatory activity against human THP-1 cells assessed as inhibition of LPS-induced IL-6 production incubated for 18 hrs by ELISA Antiinflammatory activity against human THP-1 cells assessed as inhibition of LPS-induced IL-6 production incubated for 18 hrs by ELISA	[PMID: 35318165]
TK-10	GI₅₀	1.6 μM Compound: 7; PFI-1	Growth inhibition of human TK10 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human TK10 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
U-251	GI₅₀	15.1 μM Compound: 7; PFI-1	Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
U-937	IC₅₀	> 20 μM Compound: 1; PFI-1	Cytotoxicity against human U937 cells assessed as decrease in cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as decrease in cell viability after 24 hrs by MTT assay	[PMID: 27266999]
UACC-257	GI₅₀	3.6 μM Compound: 7; PFI-1	Growth inhibition of human UACC257 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human UACC257 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
UACC-62	GI₅₀	0.7 μM Compound: 7; PFI-1	Growth inhibition of human UACC62 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human UACC62 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
UO-31	GI₅₀	1.1 μM Compound: 7; PFI-1	Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay	[PMID: 29776834]
WI-38	IC₅₀	19.8 μM Compound: PFI-1	Antiproliferative activity against human WI-38 cells incubated for 72 hrs by MTT assay Antiproliferative activity against human WI-38 cells incubated for 72 hrs by MTT assay	[PMID: 32883643]

體外研究
(In Vitro)

PFI-1對(duì)白血病細(xì)胞系具有抗增殖作用，并能有效抑制其克隆生長(zhǎng)。敏感細(xì)胞系暴露于PFI-1后會(huì)導(dǎo)致G1期細(xì)胞周期停滯、MYC表達(dá)下調(diào)以及誘導(dǎo)細(xì)胞凋亡，并促使原發(fā)性白血病母細(xì)胞分化。暴露于PFI-1的細(xì)胞顯示出Aurora B激酶顯著下調(diào)，從而減弱了Aurora底物H3S10的磷酸化，這為特異性抑制這一公認(rèn)的腫瘤學(xué)靶點(diǎn)提供了另一種策略^[1]。^[2] PFI-1與環(huán)腺苷酸反應(yīng)結(jié)合蛋白結(jié)合，解離常數(shù)K_d為49 μM。PFI-1抑制LPS刺激的人血液?jiǎn)魏思?xì)胞產(chǎn)生IL-6的EC₅₀值為1.89 μM^[2]。^[2] PFI-1在T4302 CD133⁺細(xì)胞中引起劑量依賴性的細(xì)胞存活率下降^[3]。^[2] PFI-1能夠抑制三種神經(jīng)內(nèi)分泌腫瘤（NET）細(xì)胞系的增殖，包括源自胰腺NET的Bon-1細(xì)胞系以及源自肺部NET的H727和H720細(xì)胞系^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

在大鼠中給予 PFI-1 (1 mg/kg，iv) 導(dǎo)致分布容積為 1 L/kg，血漿清除率為 18 mL/min/kg，半衰期為 1 小時(shí)。大鼠口服 PFI-1 (2 mg/kg) 導(dǎo)致口服生物利用度低至 32%。在小鼠中給予 PFI-1 (2 mg/kg，sc) 導(dǎo)致 Cmax 為 58 ng/mL，Tmax 為 1 小時(shí)，半衰期約為 2 小時(shí)^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

347.39

Formula

C₁₆H₁₇N₃O₄S

CAS 號(hào)

1403764-72-6

性狀

固體

顏色

Light yellow to yellow

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性數(shù)據(jù)

細(xì)胞實(shí)驗(yàn)：

DMSO 中的溶解度 : 33.33 mg/mL (95.94 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	2.8786 mL	14.3930 mL	28.7861 mL
5 mM	0.5757 mL	2.8786 mL	5.7572 mL
10 mM	0.2879 mL	1.4393 mL	2.8786 mL

查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

摩爾計(jì)算器
稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥方式選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

方案一

請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.20 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請(qǐng)依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.20 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實(shí)驗(yàn)周期在半個(gè)月以上的動(dòng)物實(shí)驗(yàn)酌情使用。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL玉米油中，混合均勻。

動(dòng)物溶解方案計(jì)算器

請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只

由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量

請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過(guò) 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購(gòu)。，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。

計(jì)算結(jié)果

工作液所需濃度 : mg/mL

儲(chǔ)備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。

動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過(guò)半月以上，請(qǐng)謹(jǐn)慎選擇該方案。

請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 99.87%

選擇批次：

Data Sheet (651 KB) SDS (393 KB)

COA (288 KB) LCMS (93 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻(xiàn)

[1]. Picaud S, et al. PFI-1, a Highly Selective Protein Interaction Inhibitor, Targeting BET Bromodomains. Cancer Res. 2013 May 21. [Epub ahead of print] [Content Brief]

[2]. Fish PV, et al. Identification of a chemical probe for bromo and extra C-terminal bromodomain inhibition through optimization of a fragment-derived hit. J Med Chem. 2012 Nov 26;55(22):9831-7. [Content Brief]

[3]. Cheng Z, et al. Inhibition of BET bromodomain targets genetically diverse glioblastoma. Clin Cancer Res. 2013 Apr 1;19(7):1748-59. [Content Brief]

[4]. Kate E Lines, et al. Epigenetic modifiers reduce proliferation of human neuroendocrine tumour cell lines. Endocrine Abstracts (2013) 31 P149

PFI-1 相關(guān)分類

完整儲(chǔ)備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8786 mL	14.3930 mL	28.7861 mL	71.9652 mL
	5 mM	0.5757 mL	2.8786 mL	5.7572 mL	14.3930 mL
	10 mM	0.2879 mL	1.4393 mL	2.8786 mL	7.1965 mL
	15 mM	0.1919 mL	0.9595 mL	1.9191 mL	4.7977 mL
	20 mM	0.1439 mL	0.7197 mL	1.4393 mL	3.5983 mL
	25 mM	0.1151 mL	0.5757 mL	1.1514 mL	2.8786 mL
	30 mM	0.0960 mL	0.4798 mL	0.9595 mL	2.3988 mL
	40 mM	0.0720 mL	0.3598 mL	0.7197 mL	1.7991 mL
	50 mM	0.0576 mL	0.2879 mL	0.5757 mL	1.4393 mL
	60 mM	0.0480 mL	0.2399 mL	0.4798 mL	1.1994 mL
	80 mM	0.0360 mL	0.1799 mL	0.3598 mL	0.8996 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PFI-11403764-72-6PFI1PFI 1Epigenetic Reader DomainAutophagyApoptosisInhibitorinhibitorinhibit

產(chǎn)品名稱：			* 需求量：
* 客戶姓名：			* Email：
* 電話：			* 公司或機(jī)構(gòu)名稱：
留言給我們：

產(chǎn)品名稱：			* Lot #：
* 客戶姓名：			* Email：
電話：			* 部門：
* 公司或機(jī)構(gòu)名稱：
留言給我們：

MedChemExpress (MCE) 只為有資質(zhì)的科研機(jī)構(gòu)、醫(yī)藥企業(yè)基于科學(xué)研究或藥證申報(bào)的用途提供醫(yī)藥研發(fā)服務(wù)，不為任何個(gè)人或者非科研性質(zhì)的、非用于藥證申報(bào)使用等其他用途提供服務(wù)。	站點(diǎn)地圖隱私聲明
滬ICP備15051369號(hào)-4 上海工商滬公網(wǎng)安備31011502019417 滬(浦)應(yīng)急管危經(jīng)許[2021]201709(QFYS) 營(yíng)業(yè)執(zhí)照（三證合一）
Copyright ? 2013-2025 MedChemExpress. All Rights Reserved.

成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

PFI-1

Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻(xiàn)

PFI-1 相關(guān)產(chǎn)品

•相關(guān)化合物庫(kù):

•同靶點(diǎn)產(chǎn)品:

•同靶點(diǎn)蛋白產(chǎn)品:

生物活性

純度 & 產(chǎn)品資料

參考文獻(xiàn)

摩爾計(jì)算器

稀釋計(jì)算器

PFI-1 相關(guān)分類

完整儲(chǔ)備液配制表

Keywords:

您最近查看的產(chǎn)品:

熱門區(qū)域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

姓名
郵箱 *

Sorry, but the email address you supplied was invalid.

成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

PFI-1

Customer Review

PFI-1 相關(guān)抗體

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻(xiàn)

PFI-1 相關(guān)產(chǎn)品

•相關(guān)化合物庫(kù):

•同靶點(diǎn)產(chǎn)品:

•同靶點(diǎn)蛋白產(chǎn)品:

生物活性

純度 & 產(chǎn)品資料

參考文獻(xiàn)

PFI-1 相關(guān)抗體:

摩爾計(jì)算器

稀釋計(jì)算器

PFI-1 相關(guān)分類

完整儲(chǔ)備液配制表

Keywords:

您最近查看的產(chǎn)品:

Request for HNMR Report

Request for HNMR Report

熱門區(qū)域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z