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  2. Anti-infection
  3. Antibiotic Bacterial
  4. Kanamycin

Kanamycin  (Synonyms: 卡那霉素 A; Kanamycin A)

目錄號: HY-16566 純度: 99.97%
COA 產(chǎn)品使用指南 技術(shù)支持

Kanamycin (Kanamycin A) 是一種口服有效的抗菌劑 (革蘭氏陰性/陽性細(xì)菌)，可通過與 70 S 核糖體亞單位結(jié)合，抑制轉(zhuǎn)位并導(dǎo)致編碼錯誤。Kanamycin 對 M. tuberculosis (敏感或耐藥) 以及 K. pneumonia 均具有較好的抑制活性，可用于肺結(jié)核和肺炎的研究。

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Kanamycin Chemical Structure

Kanamycin Chemical Structure

CAS No. : 59-01-8

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規(guī)格 價格 是否有貨 數(shù)量
100 mg ￥210
In-stock
200 mg ￥350
In-stock
500 mg   詢價  
1 g   詢價  

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Customer Review

Other Forms of Kanamycin:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Kanamycin (Kanamycin A) is an orally active antibacterial (gram-negative/positive bacteria) agent, inhibits translocation and causes misencoding by binding to the 70 S ribosomal subunit. Kanamycin shows good inhibitory activity to both M. tuberculosis (sensitive and drug-resistant ) and K. pneumonia, which can be used in studies of tuberculosis and pneumonia[1][2][3][4].

IC50 & Target

Aminoglycoside

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
Vero IC50
> 100 μg/mL
Compound: KAN
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay
[PMID: 24087924]
體外研究
(In Vitro)

Kanamycin (0.1-100 μg/mL; 2 weeks) exhibits good antibacterial activity (MIC=1-5 μg/mL) to various strains of mycobacteria in vitro[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: mycobacteria H37Rv, H2, H37RvR-PAS, Ravenel, Kirchbergand and BCG.
Concentration: 0.1-100 μg/mL
Incubation Time: 2 weeks
Result: Showed good antibacterial activity to various strains of mycobacteria (H37Rv, H2, H37RvR-PAS, Ravenel, and BCG) with MICs were 1 μg/mL and 5 μg/mL for strain of Kirchbergand.
體內(nèi)研究
(In Vivo)

Kanamycin (2, 4 mg/kg; s.c.; once daily, 6 times a week for 3 weeks) inhibits growth of bovine tubercle bacilli in lung and spleen of mice[1].
Kanamycin (1.25, 5 mg/kg; s.c.; single (at 3 h after infection)) inhibits the multiplication of K. pneumonia DT-S in lung, trachea, and blood of mice and in proportion to the dose administration, and also increases the survival rate of mice[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Inbred strain normal mice (14-16 g; bovine tubercle bacilli (Ravenel strain) infected model)[1].
Dosage: 2, 4 mg/kg
Administration: Subcutaneous injection; once daily, 6 times a week for 3 weeks.
Result: Exerted a marked effect to inhibit the multiplication of the tuberculosis in vivo, especially in the lung of mice.
Animal Model: Slc:ICR male mice (4-week-old; 18-24 g; K. pneumonia DT-S infection model (by the aerosol method))[2].
Dosage: 1.25, 5 mg/kg
Administration: Subcutaneous administration; single (at 3 h after infection).
Result: Suppressed the growth of K. pneumonia DT-S in lung, trachea, and blood in proportion to the dose administration.
Resulted in 90% survival at 6 days after infection (negative control group: all died within 4 days), and cleared the K. pneumonia DT-S from lung, trachea, and blood of mice within 48 h (when dosage at 5 mg/kg).
Clinical Trial
分子量

484.50

Formula

C18H36N4O11
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

卡那霉素 A
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數(shù)據(jù)
細(xì)胞實驗： 

H2O 中的溶解度 : 70 mg/mL (144.48 mM; 超聲助溶 (<60°C))

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.0640 mL 10.3199 mL 20.6398 mL
5 mM 0.4128 mL 2.0640 mL 4.1280 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
純度 & 產(chǎn)品資料

純度: 99.97%

參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.0640 mL 10.3199 mL 20.6398 mL 51.5996 mL
5 mM 0.4128 mL 2.0640 mL 4.1280 mL 10.3199 mL
10 mM 0.2064 mL 1.0320 mL 2.0640 mL 5.1600 mL
15 mM 0.1376 mL 0.6880 mL 1.3760 mL 3.4400 mL
20 mM 0.1032 mL 0.5160 mL 1.0320 mL 2.5800 mL
25 mM 0.0826 mL 0.4128 mL 0.8256 mL 2.0640 mL
30 mM 0.0688 mL 0.3440 mL 0.6880 mL 1.7200 mL
40 mM 0.0516 mL 0.2580 mL 0.5160 mL 1.2900 mL
50 mM 0.0413 mL 0.2064 mL 0.4128 mL 1.0320 mL
60 mM 0.0344 mL 0.1720 mL 0.3440 mL 0.8600 mL
80 mM 0.0258 mL 0.1290 mL 0.2580 mL 0.6450 mL
100 mM 0.0206 mL 0.1032 mL 0.2064 mL 0.5160 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Kanamycin
目錄號：
HY-16566
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