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  1. Cell Cycle/DNA Damage
  2. G-quadruplex
  3. Phen-DC3 Trifluoromethanesulfonate

Phen-DC3 Trifluoromethanesulfonate  (Synonyms: Phen-DC3 Triflate)

目錄號: HY-15594A 純度: 98.99%
COA 產品使用指南 技術支持

Phen-DC3 Trifluoromethanesulfonate 是一個 G4 特異性配體,可以抑制解旋酶 FANCJDinGIC50s 值分別為 65±6 和 50±10 nM。

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Phen-DC3 Trifluoromethanesulfonate Chemical Structure

Phen-DC3 Trifluoromethanesulfonate Chemical Structure

CAS No. : 929895-45-4

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ¥1681
In-stock
1 mg ¥600
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5 mg ¥1035
In-stock
10 mg ¥1800
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25 mg ¥3780
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50 mg 現(xiàn)貨 詢價
100 mg   詢價  
200 mg   詢價  

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Customer Review

Other Forms of Phen-DC3 Trifluoromethanesulfonate:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產品資料

  • 參考文獻

生物活性

Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively.

IC50 & Target

IC50: 65±6 nM (G4 substrate, FANCJ helicase), 50±10 nM (G4 substrate, DinG helicases)[1]

體外研究
(In Vitro)

在 WT 細胞中,CEB1-WT 陣列相當穩(wěn)定,但在添加 10 μM Phen-DC3 三氟甲磺酸鹽 (Phen-DC3) 后會頻繁重排。發(fā)現(xiàn) c-Myc 等位基因在 Phen-DC3 三氟甲磺酸鹽處理和 PIF1 缺失后表現(xiàn)出顯著的去穩(wěn)定化。CEB25-L111 (T) 陣列在 WT 細胞中穩(wěn)定,在添加 Phen-DC3 三氟甲磺酸鹽或刪除 PIF1 后變得不穩(wěn)定。在存在 Phen-DC3 三氟甲磺酸鹽或不存在 PIF1 的情況下,它也高度不穩(wěn)定。CEB1 環(huán) CEB25 等位基因在 PIF1 處理的細胞和 WT 細胞中保持完全穩(wěn)定[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

848.75

Formula

C36H26F6N6O8S2

CAS 號
性狀

固體

顏色

White to light yellow

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性數(shù)據(jù)
細胞實驗: 

DMSO 中的溶解度 : ≥ 34 mg/mL (40.06 mM; 吸濕的 DMSO 對產品的溶解度有顯著影響,請使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲備液
濃度 溶劑體積 質量 1 mg 5 mg 10 mg
1 mM 1.1782 mL 5.8910 mL 11.7820 mL
5 mM 0.2356 mL 1.1782 mL 2.3564 mL
查看完整儲備液配制表

* 請根據(jù)產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產品失效
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時,請在6個月內使用,-20°C儲存時,請在1個月內使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息:

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

由于實驗過程有損耗,建議您多配一只動物的量
計算結果
工作液所需濃度 : mg/mL
純度 & 產品資料

純度: 98.99%

參考文獻
Cell Assay
[2]

Briefly, untreated WT cells and pif1Δ cells from a fresh patch of cells get from a single colony bearing the parental allele size are diluted in 5 mL of YPD (2×105 cells/mL), grown for 8 generations at 30°C with shaking, and spreaded as single colony on YPD plates. To measure minisatellite instability upon Phen-DC3 Trifluoromethanesulfonate treatment, WT cells from a fresh patch on YPD are grown for 8 generations at 30°C in liquid SC containing Phen-DC3 Trifluoromethanesulfonate at 10 μM. Isolated colonies or pools of colonies are analyzed by Southern blot using the EcoRI digestion that cuts at each side of the minisatellite[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻

完整儲備液配制表

* 請根據(jù)產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時,請在6個月內使用,-20°C儲存時,請在1個月內使用。

可選溶劑 濃度 溶劑體積 質量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1782 mL 5.8910 mL 11.7820 mL 29.4551 mL
5 mM 0.2356 mL 1.1782 mL 2.3564 mL 5.8910 mL
10 mM 0.1178 mL 0.5891 mL 1.1782 mL 2.9455 mL
15 mM 0.0785 mL 0.3927 mL 0.7855 mL 1.9637 mL
20 mM 0.0589 mL 0.2946 mL 0.5891 mL 1.4728 mL
25 mM 0.0471 mL 0.2356 mL 0.4713 mL 1.1782 mL
30 mM 0.0393 mL 0.1964 mL 0.3927 mL 0.9818 mL
40 mM 0.0295 mL 0.1473 mL 0.2946 mL 0.7364 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產品名稱:
Phen-DC3 Trifluoromethanesulfonate
目錄號:
HY-15594A
需求量: