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  2. Cell Cycle/DNA Damage Epigenetics Autophagy
  3. Aurora Kinase Autophagy
  4. Reversine

Reversine  (Synonyms: 逆轉(zhuǎn)素)

目錄號: HY-14711 純度: 99.22%
COA 產(chǎn)品使用指南 技術(shù)支持

Reversine 是一種ATP-競爭性的 Aurora kinase 抑制劑，作用于 Aurora A，Aurora B 和 Aurora C，IC50 分別為 400，500 和 400 nM。

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Reversine Chemical Structure

Reversine Chemical Structure

CAS No. : 656820-32-5

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥716
In-stock
1 mg ￥295
In-stock
5 mg ￥651
In-stock
10 mg ￥837
In-stock
50 mg ￥2325
In-stock
100 mg ￥4185
In-stock
200 mg   詢價  
500 mg   詢價  

* Please select Quantity before adding items.

Customer Review

    Reversine purchased from MCE. Usage Cited in: Onco Targets Ther. 2018 Feb 26;11:1025-1035.  [Abstract]

    Reversine inhibits the expression levels of Cyclin A2, Cyclin B1, and p-Rb, but promotes p21 expression.

    Reversine purchased from MCE. Usage Cited in: Onco Targets Ther. 2018 Feb 26;11:1025-1035.  [Abstract]

    Reversine inhibits the expression levels of Cyclin A2, Cyclin B1, and p-Rb, but promotes p21 expression.

    Reversine purchased from MCE. Usage Cited in: Chinese Pharmacological Bulletin, 2018, 34(12): 1740-1744.

    Western analysis of Cleaved-PARP, beta-tubulin, Bcl-2, Bcl-xL, Bax protein expression in the treatment of Reversine with different concentrations.

    查看 Aurora Kinase 亞型特異性產(chǎn)品:

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Reversine is a novel class of ATP-competitive Aurora kinase inhibitor with IC50s of 400, 500 and 400 nM for Aurora A, Aurora B and Aurora C, respectively.

    IC50 & Target[1]

    Aurora A

    400 nM (IC50)

    Aurora B

    500 nM (IC50)

    Aurora C

    400 nM (IC50)

    體外研究
    (In Vitro)

    Reversine 是一種新型極光激酶抑制劑，可抑制人急性髓性白血病細胞的集落形成。Reversine 是 Aurora A 和 B 的有效抑制劑，也是 Aurora C 激酶的抑制劑。濃度為 0.5 μM時，Aurora A 和 B 活性被抑制 80%，Aurora 激酶 C 被抑制 55%，而在其他測試的激酶上沒有觀察到抑制或僅觀察到適度抑制。在第二輪實驗中，Reversine 對極光激酶 A 的 IC50 測定為 400 nM，而極光激酶 B 和 C IC50 分別為 500 和 400 nM。MEK1 的 IC50 也測定為 >1.5 μM，肌肉肌球蛋白 (非肌肉肌球蛋白 II 的類似物) 的 IC50 為 350 nM[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    Reversine 和 Aspirin 的組合可以更有效地誘導(dǎo)細胞周期停滯和細胞凋亡。為了評估這種組合的抗腫瘤作用，通過皮下注射建立了異種移植裸鼠模型。接種宮頸癌細胞的小鼠在腫瘤接種后約 16 天時體重減輕了約 10%。然而，聯(lián)合處理組的腫瘤生長 (腫瘤重量) 減少，小鼠存活時間更長[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    393.49

    Formula

    C21H27N7O
    CAS 號
    性狀

    固體

    顏色

    Light green to green

    中文名稱

    逆轉(zhuǎn)素
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : 20 mg/mL (50.83 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.5414 mL 12.7068 mL 25.4136 mL
    5 mM 0.5083 mL 2.5414 mL 5.0827 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    計算結(jié)果
    工作液所需濃度 : mg/mL
    純度 & 產(chǎn)品資料

    純度: 99.22%

    參考文獻
    Cell Assay
    [1]

    HCT116 and HL60 cells are incubated with either 5 μmol/L Reversine or DMSO 0.01%. Cells are harvested and fixed in 70% ethanol overnight. After double washing with PBS, cells are labeled with cell cycle staining reagent PBS, 0.1% Triton X-100, 200 μg/mL DNase-free RNase, and 25 μg/mL propidium iodide and incubated at room temperature in the dark for 30 min. DNA content is analyzed using FACSCalibur. Cell viability of different tumor cell lines is assessed using ATPlite 1step. Briefly, 2×104 cells for each well are plated in a 96-well plate in presence of crescent quantity of Reversine. After 72 h, the plates are recovered and 100 μL ATPlite solution is added to each well. The plates are shaken for 2 min at 700 rpm and luminescence is measured using EnVision Multilabel plate reader. Each sample is analyzed in triplicate[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    Female athymic 6-8 weeks old BALB/c nude mice are used. U14 cell suspension (5×106 cells in 100 μL of RPMI-1640 medium) is injected subcutaneously. The purpose of developing cervical tumors is to generate histological intact tumors for drug therapy. When the diameter of tumors reached up to about 1 cm, Reversine, aspirin or their combinations are administrated by intraperitoneal injection per 3 days, twenty-five of these mice are randomly assigned into one of the following five groups: (a) mice treated with RPMI-1640 medium, (b) mice treated with DMSO, (c) mice treated with Reversine (10 mg/kg), (d) mice treated with aspirin (1 μg/kg) and (e) mice treated with a Reversine and aspirin combination. Body weight and tumor size at the site of inoculation are measured three times a week. Tumor size is measured every 3 days from two diameters, tumor volume is estimated using the formula L×S2/2(L as the longest diameter, S as the shortest diameter).

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5414 mL 12.7068 mL 25.4136 mL 63.5340 mL
    5 mM 0.5083 mL 2.5414 mL 5.0827 mL 12.7068 mL
    10 mM 0.2541 mL 1.2707 mL 2.5414 mL 6.3534 mL
    15 mM 0.1694 mL 0.8471 mL 1.6942 mL 4.2356 mL
    20 mM 0.1271 mL 0.6353 mL 1.2707 mL 3.1767 mL
    25 mM 0.1017 mL 0.5083 mL 1.0165 mL 2.5414 mL
    30 mM 0.0847 mL 0.4236 mL 0.8471 mL 2.1178 mL
    40 mM 0.0635 mL 0.3177 mL 0.6353 mL 1.5884 mL
    50 mM 0.0508 mL 0.2541 mL 0.5083 mL 1.2707 mL
    Help & FAQs
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Reversine
    目錄號：
    HY-14711
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