Sulforaphane (Synonyms: 蘿卜硫素)

目錄號: HY-13755 純度: 98.87%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南

摩爾計算器

Sulforaphane 是一種具有口服活性的 Keap1/Nrf2/ARE 誘導(dǎo)劑。Sulforaphane 能促進腫瘤抑制蛋白的轉(zhuǎn)錄，并有效地抑制 HDACs 的活性。Sulforaphane 通過激活 Keap1/Nrf2/ARE 途徑，以及進一步誘導(dǎo) HO-1 的表達保護心臟。Sulforaphane 通過 AMPK 依賴性信號傳導(dǎo)抑制高糖誘導(dǎo)的胰腺癌。Sulforaphane 具有抗癌和抗炎活性。

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Sulforaphane Chemical Structure

CAS No. : 4478-93-7

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規(guī)格	價格	是否有貨
5 mg	￥375	In-stock
10 mg	￥600	In-stock
25 mg	￥1350	In-stock
50 mg	￥2500	In-stock
100 mg	￥4500	In-stock
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500 mg		詢價

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Other Forms of Sulforaphane:

(R)-Sulforaphane In-stock

MCE 顧客使用本產(chǎn)品發(fā)表的 45 篇科研文獻

•J Adv Res. 2024 Jul 26:S2090-1232(24)00307-2. [Abstract]
•Nat Commun. 2023 Sep 18;14(1):5778. [Abstract]
•Acta Pharm Sin B. 2021 May;11(5):1246-1260.
•Theranostics. 2020 Jun 5;10(16):7319-7334. [Abstract]
•Acta Pharmacol Sin. 2021 Jul 16. [Abstract]
•Eur J Cell Biol. 2024 Oct 18;103(4):151463. [Abstract]

•Phytomedicine. 2024 Oct:133:155874. [Abstract]
•Phytomedicine. 2024 Feb 10:126:155441. [Abstract]
•Acta Neuropathol Commun. 2023 Oct 24;11(1):171. [Abstract]
•J Anim Sci Biotechnol. 2023 May 3;14(1):61. [Abstract]
•J Agric Food Chem. 2023 Jan 5. [Abstract]
•Free Radic Biol Med. 2022 Oct 14;193(Pt 1):177-189. [Abstract]
•Biomed Pharmacother. 2021 Jun 8;111652. [Abstract]
•Transl Psychiatry. 2021 Feb 24;11(1):140. [Abstract]
•Phytomedicine. 2021 Jan;80:153370. [Abstract]
•Life Sci. 2020 Dec 15;263:118485. [Abstract]
•BBA-Mol Basis Dis. 2020 Jan 1;1866(1):165575. [Abstract]
•J Ethnopharmacol. 2024 Aug 31:118776. [Abstract]
•Antioxid Redox Signal. 2024 May 30. [Abstract]
•Biochem Pharmacol. 2024 Jun 4:226:116337. [Abstract]
•Biomedicines. 2024 Apr 30, 12(5), 991.
•Antioxidants (Basel). 2023 Apr 28, 12(5), 1022.
•Int J Mol Sci. 2023, 24(2), 1070.
•J Sci Food Agric. 2022 Dec 9. [Abstract]
•J Nutr Biochem. 2022 Nov 23;109226. [Abstract]
•Nutrients. 2022, 14(19), 4017.
•Eur J Pharmacol. 2021 Aug 7;174412. [Abstract]
•J Neurochem. 2021 Jul 13. [Abstract]
•Ren Fail. 2024 Dec;46(2):2416937. [Abstract]
•Tohoku J Exp Med. 2024 Oct 3. [Abstract]
•Biosci Rep. 2024 Jun 13:BSR20240084. [Abstract]
•Redox Rep. 2023 Dec;28(1):2279818. [Abstract]
•Psychopharmacology. 2023 Feb 27. [Abstract]
•ChemMedChem. 2022 Jan 31;e202100732. [Abstract]
•Mol Biol Rep. 2021 Nov 3. [Abstract]
•J Cancer Res Clin Oncol. 2019 Apr;145(4):861-872. [Abstract]
•Vascul Pharmacol. 2018 Oct;109:56-71. [Abstract]
•Transpl Immunol. 2021, 101439.
•Res Sq. 2024 Aug 13.
•Sports Med Health Sci. 2024 Apr 4.
•Research Square Preprint. 2023 May 11.
•Research Square Preprint. 2023 Apr 6.
•Research Square Print. 2022 Jul.
•Research Square Preprint. 2021 Jan.
•Research Square Preprint. 2020 Nov.

: Sulforaphane purchased from MCE. Usage Cited in: Eur J Pharmacol. 2021 Aug 7;174412. [Abstract]; Western blotting analysis shows that CIBP can significantly induce the protein expression of Nrf2 and HO-1 in the spinal cord and that intrathecal administration of Sulforaphane (SFN) can further promoted the protein expression of Nrf2 and HO-1 protein.

: Sulforaphane purchased from MCE. Usage Cited in: Eur J Pharmacol. 2021 Aug 7;174412. [Abstract]; Immunofluorescence histochemistry confirmes that Sulforaphane (SFN) treatment further increased Nrf2 and HO-1 expression in the dorsal horn of the spinal cord of CIBP rats.

: Sulforaphane purchased from MCE. Usage Cited in: Theranostics. 2020 Jun 5;10(16):7319-7334. [Abstract]; Western blot detection of Nrf2, TLR4, IRF1, iNOS, and ARG-1 levels following Sulforaphane (SFN; 10 μM or 20 μM) treatment or Nrf2 upregulation/downregulation in BMDMs co-cultured with COM-stimulated TECs. SFN treatment activates Nrf2 expression, which then downregulates the expression of TLR4, IRF1, and iNOS and upregulates the expression of ARG-1.

: Sulforaphane purchased from MCE. Usage Cited in: Theranostics. 2020 Jun 5;10(16):7319-7334. [Abstract]; The distribution of iNOS (green) and ARG-1 (red) in BMDMs according to immunofluorescence. Sulforaphane (SFN; 10 μM or 20 μM) decreases iNOS and increases ARG-1 levels.

: Sulforaphane purchased from MCE. Usage Cited in: Vascul Pharmacol. 2018 Oct;109:56-71. [Abstract]; A representative western blot analysis gel showing the reduces abundance of eNOS relative to GAPDH in tissues cultured under hyperglycaemic conditions (25 mM glucose: 25) versus either freshly isolated tissues (F, first lane on left) or tissues cultured under euglycaemic conditions (10 mM glucose: 10). The abundance of eNOS is preserved for tissues cultured in 25 mM glucose along with 1 μM Sulforaphane (25+S).

Sulforaphane 相關(guān)產(chǎn)品

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生物活性
純度 & 產(chǎn)品資料
參考文獻

生物活性

Sulforaphane is an orally active inducer of the Keap1/Nrf2/ARE pathway. Sulforaphane promotes the transcription of tumor-suppressing proteins and effectively inhibits the activity of HDACs. Through the activation of the Keap1/Nrf2/ARE pathway and further induction of HO-1 expression, Sulforaphane protects the heart. Sulforaphane suppresses high glucose-induced pancreatic cancer through AMPK-dependent signal transmission. Sulforaphane exhibits both anticancer and anti-inflammatory properties^[1]^[2]^[3]^[4]^[5]^[6].

IC₅₀ & Target^[1]

HDAC

Bax

Caspase-3

細胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-375	IC₅₀	24.52 μM Compound: SFA	Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A375 cells after 72 hrs by CCK8 assay	[PMID: 30282324]
A-431	IC₅₀	15.76 μM Compound: SFN	Antiproliferative activity against human A431 cells assessed as cell growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human A431 cells assessed as cell growth inhibition after 72 hrs by MTT assay	[PMID: 27135370]
A-431	IC₅₀	9 μM Compound: SFN	Inhibition of EGFR-TK in human A431 cell lysate assessed as reduction in EGF stimulated kinase activity after 60 mins using biotinylated peptide substrate by ELISA Inhibition of EGFR-TK in human A431 cell lysate assessed as reduction in EGF stimulated kinase activity after 60 mins using biotinylated peptide substrate by ELISA	[PMID: 27135370]
A549	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human A549 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human A549 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
A549	IC₅₀	11.93 μM Compound: SFA	Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay	[PMID: 30282324]
A549	IC₅₀	20.18 μM Compound: SFN	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
A549	IC₅₀	21.99 μM Compound: 77, SFN	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 23685571]
AGS	IC₅₀	18.41 μM Compound: SFN	Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human AGS cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
ARPE-19	IC₅₀	10 μM Compound: Sulforaphane	Cytotoxicity against human ARPE-19 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human ARPE-19 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 35549469]
Caco-2	IC₅₀	79.83 μM Compound: SF, Sulforaphane	Cytotoxicity against human Caco2 cells incubated for 24 hrs by WST1 dye reduction assay Cytotoxicity against human Caco2 cells incubated for 24 hrs by WST1 dye reduction assay	[PMID: 22998472]
CCRF-CEM	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human CCRF-CEM cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human CCRF-CEM cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
DU-145	IC₅₀	10 μM Compound: 1	Antiproliferative activity against human DU145 cells assessed as viable cells after 24 hrs Antiproliferative activity against human DU145 cells assessed as viable cells after 24 hrs	[PMID: 18855447]
DU-145	IC₅₀	13.8 μM Compound: SF, Sulforaphane	Cytotoxicity against human DU145 cells incubated for 24 hrs by WST1 dye reduction assay Cytotoxicity against human DU145 cells incubated for 24 hrs by WST1 dye reduction assay	[PMID: 22998472]
HCC827	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human HCC827 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human HCC827 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
HCT-116	IC₅₀	11.59 μM Compound: 77, SFN	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 23685571]
HCT-116	IC₅₀	36.73 μM Compound: SFA	Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay	[PMID: 30282324]
HeLa	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human HeLa cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
HeLa	IC₅₀	66.09 μM Compound: SFA	Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay Antiproliferative activity against human HeLa cells after 72 hrs by CCK8 assay	[PMID: 30282324]
Hep 3B2	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human Hep3B cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human Hep3B cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
HepG2	EC₅₀	1.2 μM Compound: Sulforaphane	Activation of Nrf2 (unknown origin) expressed in human HepG2 cells after 5 hrs by ARE-driven luciferase reporter gene assay Activation of Nrf2 (unknown origin) expressed in human HepG2 cells after 5 hrs by ARE-driven luciferase reporter gene assay	[PMID: 28126440]
HepG2	IC₅₀	14.05 μM Compound: 77, SFN	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 23685571]
HepG2	IC₅₀	20.99 μM Compound: SFN	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
HepG2	IC₅₀	24.89 μM Compound: SF, Sulforaphane	Cytotoxicity against human HepG2 cells incubated for 24 hrs by WST1 dye reduction assay Cytotoxicity against human HepG2 cells incubated for 24 hrs by WST1 dye reduction assay	[PMID: 22998472]
HepG2	EC₅₀	4 μM Compound: sulforaphane	Induction of ARE in human HepG2 cells by luciferase assay Induction of ARE in human HepG2 cells by luciferase assay	[PMID: 16562850]
HepG2	IC₅₀	8.357 μM Compound: SFN	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
HONE1 cell line	IC₅₀	19.26 μM Compound: SFN	Antiproliferative activity against human HONE1 cell line assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human HONE1 cell line assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
Jurkat	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human Jurkat cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human Jurkat cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
Jurkat	IC₅₀	15 μM Compound: SFN	Cytotoxicity against human Jurkat T cells assessed as decrease in cell viability after 24 hrs by flow cytometric analysis Cytotoxicity against human Jurkat T cells assessed as decrease in cell viability after 24 hrs by flow cytometric analysis	[PMID: 22200402]
Jurkat	IC₅₀	3.8 μM Compound: 1	Antiproliferative activity against human lymphoblastoid cells using annexin-V FITC and PI staining by flow cytometry Antiproliferative activity against human lymphoblastoid cells using annexin-V FITC and PI staining by flow cytometry	[PMID: 18855447]
LoVo	IC₅₀	18.52 μM Compound: SFN	Antiproliferative activity against human LoVo cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human LoVo cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
LoVo	IC₅₀	22.9 μM Compound: SFN	Antiproliferative activity against human LoVo cells after 72 hrs by SRB assay Antiproliferative activity against human LoVo cells after 72 hrs by SRB assay	[PMID: 29945793]
LoVo	IC₅₀	22.9 μM Compound: SFN	Cytotoxicity against human LoVo cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human LoVo cells after 72 hrs by sulforhodamine B assay	[PMID: 28342398]
MCF7	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
MCF7	IC₅₀	17.66 μM Compound: 77, SFN	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 23685571]
MCF7	IC₅₀	23.13 μM Compound: SFN	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
MDA-MB-231	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
MDA-MB-231	IC₅₀	22.18 μM Compound: SFN	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
NCI-H1299	IC₅₀	11.57 μM Compound: SFN	Antiproliferative activity against human NCI-H1299 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human NCI-H1299 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
NCI-H1299	IC₅₀	7.648 μM Compound: SFN	Antiproliferative activity against human H1299 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human H1299 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
NCI-H1650	IC₅₀	22.01 μM Compound: SFN	Antiproliferative activity against human NCI-H1650 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human NCI-H1650 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
NCI-H1975	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
NCI-H460	IC₅₀	21.25 μM Compound: SFN	Antiproliferative activity against human NCI-H460 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human NCI-H460 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
OVCAR-3	IC₅₀	18.44 μM Compound: SFN	Antiproliferative activity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human OVCAR-3 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
PC-3	IC₅₀	32.38 μM Compound: SF, Sulforaphane	Cytotoxicity against human PC3 cells incubated for 24 hrs by WST1 dye reduction assay Cytotoxicity against human PC3 cells incubated for 24 hrs by WST1 dye reduction assay	[PMID: 22998472]
PC-9	IC₅₀	6.308 μM Compound: SFN	Antiproliferative activity against human PC9 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human PC9 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
Peritoneal macrophage	CC₅₀	> 10 μM Compound: SFN	Cytotoxicity against mouse peritoneal macrophages assessed as reduction in cell viability measured after 24 hrs by CCK8 assay Cytotoxicity against mouse peritoneal macrophages assessed as reduction in cell viability measured after 24 hrs by CCK8 assay	[PMID: 34119834]
RAW264.7	IC₅₀	400 nM Compound: sulforaphane	Inhibition of iNOS in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of nitric oxide production after 48 hrs by Griess assay Inhibition of iNOS in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of nitric oxide production after 48 hrs by Griess assay	[PMID: 25965897]
SGC-7901	IC₅₀	24.95 μM Compound: SFN	Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
SH-SY5Y	IC₅₀	13.27 μM Compound: 77, SFN	Cytotoxicity against human SH-SY5Y cells after 72 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells after 72 hrs by MTT assay	[PMID: 23685571]
SH-SY5Y	IC₅₀	20 μM Compound: sulforaphane	Cytotoxicity against human SH-SY5Y cells after 24 hrs by Annexin V-PE-based flow cytometry Cytotoxicity against human SH-SY5Y cells after 24 hrs by Annexin V-PE-based flow cytometry	[PMID: 24140951]
SiHa	IC₅₀	9.953 μM Compound: SFN	Antiproliferative activity against human SiHa cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human SiHa cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
SMMC-7721	IC₅₀	7.87 μM Compound: SFA	Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay Antiproliferative activity against human SMMC7721 cells after 72 hrs by CCK8 assay	[PMID: 30282324]
SW480	IC₅₀	17.84 μM Compound: SFN	Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
U-251	IC₅₀	22.47 μM Compound: SFN	Antiproliferative activity against human U-251 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human U-251 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 32416457]
U2OS	EC₅₀	0.58 μM Compound: SFN	Activation of ED-tagged Nrf2 in human U2OS cells co-expressing Keap1 assessed as induction of NRF2 translocation to nucleus after 6 hrs by beta-galactosidase based chemiluminescent assay Activation of ED-tagged Nrf2 in human U2OS cells co-expressing Keap1 assessed as induction of NRF2 translocation to nucleus after 6 hrs by beta-galactosidase based chemiluminescent assay	[PMID: 31312409]
U2OS	EC₅₀	0.58 μM Compound: SFN	Activation of NRF2 in human U2OS cells co-expressing Keap1 assessed as induction of NRF2 translocation to nucleus after 6 hrs by beta-galactosidase based chemiluminescent assay Activation of NRF2 in human U2OS cells co-expressing Keap1 assessed as induction of NRF2 translocation to nucleus after 6 hrs by beta-galactosidase based chemiluminescent assay	[PMID: 30540174]
U2OS	EC₅₀	0.87 μM Compound: SFN	Activation of NRF2 in human U2OS cells co-expressing Keap1 (unknown origin) assessed as induction of NRF2 translocation to nucleus incubated for 6 hrs by beta-galactosidase based chemiluminescent assay Activation of NRF2 in human U2OS cells co-expressing Keap1 (unknown origin) assessed as induction of NRF2 translocation to nucleus incubated for 6 hrs by beta-galactosidase based chemiluminescent assay	[PMID: 31670201]
U-937	IC₅₀	> 10 μM Compound: SFN	Antiproliferative activity against human U937 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay Antiproliferative activity against human U937 cells assessed as inhibition of cell viability after 72 hrs by CCK-8 assay	[PMID: 31376566]
Vero	IC₅₀	13.72 μM Compound: 77, SFN	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23685571]

體外研究
(In Vitro)

Sulforaphane (0-30 μM) 以劑量依賴性方式引發(fā)細胞周期停滯和細胞凋亡 (apoptosis)。這種由 Sulforaphane 誘導(dǎo)的細胞周期停滯與細胞周期蛋白 A 和 B1 的表達增加有關(guān)^[1]。
Sulforaphane (0-30 μM) 能抑制 HT29 細胞的生長再啟動，降低細胞活性，對分化的細胞具有較低的毒性^[1]。
Sulforaphane (10 μM, 24 小時) 預(yù)處理 H9c2 細胞后，可以減少凋亡細胞數(shù)量，降低促凋亡蛋白 (Bax、caspase-3、細胞色素 c) 的表達，并抵消 Doxorubicin (HY-15142A) (1 μM, 2 小時) 引發(fā)的線粒體膜電位增加^[2]。
Sulforaphane (10 μM, 2 或 24 小時) 通過激活 Keap1/Nrf2/ARE 途徑，以及進一步誘導(dǎo) HO-1 的表達，能有效地減少 Doxorubicin (1 μM, 2 或 24 小時) 誘導(dǎo)的 H9c2 細胞中 ROS 的產(chǎn)生和細胞凋亡^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	H9c2 cells
Concentration:	Sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	Sulforaphane: 2h; Doxorubicin: 2, 24h
Result:	Prevented the release of cytochrome c into the cytosol. Prevented the translocation of Bax into the cytosol. Attenuated the doxorubicin-induced increase in the levels of cleaved caspase-3. Induced a significant increase in HO-1 protein expression. Induced a significantly higher level of Nrf2 expression in the nucleus compared to the cytoplasm.

Apoptosis Analysis^[2]

Cell Line:	H9c2 cells
Concentration:	Sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	Sulforaphane: 2h; Doxorubicin: 24h
Result:	Protected the H9c2 cells against doxorubicin-induced cell death. Increased cell viability in a dose-dependent manner. Significantly reduced the number of apoptotic cells treated with Doxorubicin.

RT-PCR^[2]

Cell Line:	H9c2 cells
Concentration:	Sulforaphane: 10 μM; Doxorubicin: 1 μM
Incubation Time:	Sulforaphane: 2, 24h; Doxorubicin: 2, 24h
Result:	Induced heme oxygenase-1 (HO-1) mRNA expression in a dose-dependent manner.

體內(nèi)研究
(In Vivo)

Sulforaphane (13.3, 17.7, 26.6 mg/kg; 灌胃; 5 天) 能抑制雌性 Sprague-Dawley 大鼠接受 DMBA (HY-W011845) (8 mg/mL) 單次劑量處理后乳腺腫瘤的形成^[3]。
Sulforaphane (13.3, 26.6 mg/kg; 灌胃; 5 天) 能減少由 DMBA (8 mg/mL) 在雌性 Sprague-Dawley 大鼠中誘發(fā)的乳腺腫瘤的發(fā)生率、多發(fā)性和重量，并延遲其發(fā)展^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Sprague-Dawley rat^[3]
Dosage:	Sulforaphane: 13.3, 17.7, 26.6 mg/kg; DMBA (HY-W011845): 8 mg/mL
Administration:	Oral gavage; 5 days
Result:	Prevented the occurrence of tumors in a dose-dependent manner. Significantly reduced the incidence of tumors.

Clinical Trial

分子量

177.29

Formula

C₆H₁₁NOS₂

CAS 號

4478-93-7

性狀

液體（密度：1.17±0.1 g/cm³）

顏色

Colorless to light yellow

中文名稱

蘿卜硫素；萊菔硫烷

結(jié)構(gòu)分類

Others

初始來源

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

-20°C, sealed storage, away from moisture and light

*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

溶解性數(shù)據(jù)

細胞實驗：

DMSO 中的溶解度 : ≥ 62.5 mg/mL (352.53 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	5.6405 mL	28.2024 mL	56.4048 mL
5 mM	1.1281 mL	5.6405 mL	11.2810 mL
10 mM	0.5640 mL	2.8202 mL	5.6405 mL

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實驗：

請根據(jù)您的實驗動物和給藥方式選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.10 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.10 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液中，混合均勻。
2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
方案三

請依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.10 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

方案一

請依序添加每種溶劑： 50% PEG300 50% Saline
Solubility: ≥ 50 mg/mL (282.02 mM); 澄清溶液
方案二

請依序添加每種溶劑： 0.5% CMC-Na/saline water
Solubility: ≥ 50 mg/mL (282.02 mM); 澄清溶液
方案三

請依序添加每種溶劑： 30% SBE-β-CD in Saline
Solubility: 10 mg/mL (56.40 mM); 澄清溶液; 超聲助溶

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

請輸入您的動物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計算結(jié)果

工作液所需濃度 : mg/mL

儲備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。

動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。

請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 99.75%

選擇批次：

Data Sheet (647 KB) SDS (421 KB)

COA (190 KB) HNMR (191 KB) LCMS (139 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻

[1]. Gamet-Payrastre L, et al. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 2000 Mar 1;60(5):1426-33. [Content Brief]

[2]. Li B, et al. Sulforaphane prevents doxorubicin-induced oxidative stress and cell death in rat H9c2 cells. Int J Mol Med. 2015 Jul;36(1):53-64. [Content Brief]

[3]. Zhang Y, et al. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornylisothiocyanates. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3147-50. [Content Brief]

[4]. Chen X, et al. Activation of Nrf2 by Sulforaphane Inhibits High Glucose-Induced Progression of PancreaticCancer via AMPK Dependent Signaling. ell Physiol Biochem. 2018;50(3):1201-1215. [Content Brief]

[5]. De Nicola GR, et al. Novel gram-scale production of enantiopure R-sulforaphane from Tuscan black kale seeds. Molecules. 2014 May 27;19(6):6975-86. [Content Brief]

[6]. Abdull Razis AF, et al. The natural chemopreventive phytochemical R-sulforaphane is a far more potent inducer of the carcinogen-detoxifying enzyme systems in rat liver and lung than the S-isomer. Int J Cancer. 2011 Jun 15;128(12):2775-82. [Content Brief]

Sulforaphane 相關(guān)分類

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6405 mL	28.2024 mL	56.4048 mL	141.0119 mL
	5 mM	1.1281 mL	5.6405 mL	11.2810 mL	28.2024 mL
	10 mM	0.5640 mL	2.8202 mL	5.6405 mL	14.1012 mL
	15 mM	0.3760 mL	1.8802 mL	3.7603 mL	9.4008 mL
	20 mM	0.2820 mL	1.4101 mL	2.8202 mL	7.0506 mL
	25 mM	0.2256 mL	1.1281 mL	2.2562 mL	5.6405 mL
	30 mM	0.1880 mL	0.9401 mL	1.8802 mL	4.7004 mL
	40 mM	0.1410 mL	0.7051 mL	1.4101 mL	3.5253 mL
	50 mM	0.1128 mL	0.5640 mL	1.1281 mL	2.8202 mL
	60 mM	0.0940 mL	0.4700 mL	0.9401 mL	2.3502 mL
	80 mM	0.0705 mL	0.3525 mL	0.7051 mL	1.7626 mL
	100 mM	0.0564 mL	0.2820 mL	0.5640 mL	1.4101 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Sulforaphane (Synonyms: 蘿卜硫素)

Customer Review

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MCE 顧客使用本產(chǎn)品發(fā)表的 45 篇科研文獻

Sulforaphane 相關(guān)產(chǎn)品

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生物活性

純度 & 產(chǎn)品資料

參考文獻

摩爾計算器

稀釋計算器

Sulforaphane 相關(guān)分類

完整儲備液配制表

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Sulforaphane (Synonyms: 蘿卜硫素)

Customer Review

Other Forms of Sulforaphane:

Sulforaphane 相關(guān)抗體

MCE 顧客使用本產(chǎn)品發(fā)表的 45 篇科研文獻

Sulforaphane 相關(guān)產(chǎn)品

•相關(guān)化合物庫:

•同靶點產(chǎn)品:

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查看 HDAC 亞型特異性產(chǎn)品:

查看 Bcl-2 Family 亞型特異性產(chǎn)品:

查看 Caspase 亞型特異性產(chǎn)品:

生物活性

純度 & 產(chǎn)品資料

參考文獻

Sulforaphane 相關(guān)抗體:

摩爾計算器

稀釋計算器

Sulforaphane 相關(guān)分類

完整儲備液配制表

Keywords:

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