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  2. 誘導(dǎo)疾病模型產(chǎn)品 Metabolic Enzyme/Protease Neuronal Signaling GPCR/G Protein Autophagy
  3. 心血管系統(tǒng)疾病模型 Endogenous Metabolite Adrenergic Receptor Autophagy
  4. 心臟疾病模型
  5. Norepinephrine

Norepinephrine  (Synonyms: 去甲腎上腺素; Levarterenol; L-Noradrenaline)

目錄號(hào): HY-13715 純度: 99.43%
COA 產(chǎn)品使用指南 技術(shù)支持

Norepinephrine (Levarterenol; L-Noradrenaline) 是一種有效的腎上腺素能受體 (AR) 激動(dòng)劑。Norepinephrine 可以激活 α1、α2、β1 受體。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Norepinephrine Chemical Structure

Norepinephrine Chemical Structure

CAS No. : 51-41-2

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Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 37 篇科研文獻(xiàn)

IF
WB

    Norepinephrine purchased from MCE. Usage Cited in: Cardiovasc Res. 2018 Feb 1;114(2):300-311.  [Abstract]

    Knockdown of GATA4 ameliorated morphology and functional changes in a Meox1 cell line with NE stimulation. Immunodetection of actin in HL-1 cells. Actin staining appeared green, and sections are counterstained blue with DAPI (40,6-diamidino-2-phenylindole).

    Norepinephrine purchased from MCE. Usage Cited in: J Nutr Biochem. 2018 May 1;58:110-118.  [Abstract]

    PASMCs are pretreated with or not Norepinephrine (50 μM) or ICI 118,551 (5 nM), and α-SMA (D) are analyzed.

    查看 Adrenergic Receptor 亞型特異性產(chǎn)品:

    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Norepinephrine (Levarterenol; L-Noradrenaline) is a potent adrenergic receptor (AR) agonist. Norepinephrine activates α1, α2, β1 receptors[1][2][3][4].

    IC50 & Target[1][2]

    α1-adrenergic receptor

     

    α2-adrenergic receptor

     

    Beta-1 adrenergic receptor

     

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    3T3-L1 IC50
    > 100 μg/mL
    Compound: (-)-Arterenol
    Cytotoxicity against mouse 3T3L1 cells assessed as cell viability after 7 days
    Cytotoxicity against mouse 3T3L1 cells assessed as cell viability after 7 days
    		[PMID: 20052971]
    3T3-L1 IC50
    69 μg/mL
    Compound: (-)-Arterenol
    Inhibition of adipogenesis in mouse 3T3L1 cells assessed as triglyceride accumulation after 7 days
    Inhibition of adipogenesis in mouse 3T3L1 cells assessed as triglyceride accumulation after 7 days
    		[PMID: 20052971]
    CHO EC50
    110 nM
    Compound: Noradrenaline
    Agonist activity at human recombinant alpha2A adrenergic receptor expressed in CHO cells assessed as induction of [35S]GTPgammaS binding after 60 mins by scintillation counting
    Agonist activity at human recombinant alpha2A adrenergic receptor expressed in CHO cells assessed as induction of [35S]GTPgammaS binding after 60 mins by scintillation counting
    		[PMID: 21129985]
    CHO EC50
    5.5 nM
    Compound: norepinephrine
    Agonist activity at human adrenergic beta-3 receptor expressed in CHO cells assessed as cAMP levels by DELFIA method
    Agonist activity at human adrenergic beta-3 receptor expressed in CHO cells assessed as cAMP levels by DELFIA method
    		[PMID: 18083578]
    CHO EC50
    6.3 nM
    Compound: norepinephrine
    Agonist activity at human adrenergic beta3 receptor expressed in CHO cells assessed as [3H]cAMP levels by radiolabeled ligand based assay
    Agonist activity at human adrenergic beta3 receptor expressed in CHO cells assessed as [3H]cAMP levels by radiolabeled ligand based assay
    		[PMID: 18083578]
    CHO-K1 EC50
    118 nM
    Compound: Noradrenaline
    Agonist activity at human adrenoceptor alpha2A expressed in CHOK1 cells assessed as stimulation of agonist-induced [35S]GTPgammaS binding by scintillation counting
    Agonist activity at human adrenoceptor alpha2A expressed in CHOK1 cells assessed as stimulation of agonist-induced [35S]GTPgammaS binding by scintillation counting
    		[PMID: 22172308]
    CHO-K1 EC50
    166 nM
    Compound: Noradrenaline
    Agonist activity at human recombinant Alpha-2 adrenergic receptor expressed in CHOK1 cells after 30 mins by [35S]GTPgammaS binding assay
    Agonist activity at human recombinant Alpha-2 adrenergic receptor expressed in CHOK1 cells after 30 mins by [35S]GTPgammaS binding assay
    		[PMID: 27031216]
    HEK293 EC50
    9.1 nM
    Compound: norepinephrine
    Agonist activity at human alpha1A-adrenergic receptor expressed in HEK293 cells assessed as calcium flux by fluorescence assay
    Agonist activity at human alpha1A-adrenergic receptor expressed in HEK293 cells assessed as calcium flux by fluorescence assay
    		[PMID: 26125514]
    HEK293 EC50
    9.1 nM
    Compound: Norepinephrine
    Agonist activity at recombinant human alpha1A adrenergic receptor expressed in HEK293 cells assessed as intercellular Ca mobilization by Fura-2AM dye-based fluorescence assay
    Agonist activity at recombinant human alpha1A adrenergic receptor expressed in HEK293 cells assessed as intercellular Ca mobilization by Fura-2AM dye-based fluorescence assay
    		[PMID: 28355078]
    體外研究
    (In Vitro)

    Norepinephrine (NE) 通常被認(rèn)為是一種 β1-亞型選擇性腎上腺素能激動(dòng)劑，優(yōu)于 β2-腎上腺素能受體。Norepinephrine (NE) 在較高濃度下也對 β2-腎上腺素能受體具有直接活性[1]。
    來自腹股溝脂肪墊 (iWA) 或肩胛間脂肪墊 (BA) 從新生野生型 C57BL/6J 小鼠中分離并培養(yǎng)。為了檢查激活 AT2 對 β-腎上腺素能信號(hào)傳導(dǎo)的影響，首先評估 cAMP 產(chǎn)生對 Norepinephrine (NE，10 μM) 有或沒有 CGP (10 nM) 聯(lián)合處理的反應(yīng)。
    Norepinephrine (NE) 增加 cAMP正如在 iWA 中預(yù)期的那樣，CGP 不會(huì)改變這種效果
    Norepinephrine (NE) 也已知會(huì)誘導(dǎo)脂肪分解，并且需要釋放的脂肪酸來功能性激活 UCP1 蛋白并刺激熱量產(chǎn)生。在小鼠 iWA 中，Norepinephrine (NE) 處理后 Ser133 處的 CREB 磷酸化增加，并且與 CGP 聯(lián)合處理顯著減弱[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    誘導(dǎo)心肌病[5][6]

    致病原理
    Norepinephrine 是心肌細(xì)胞的一種強(qiáng)效生長因子，通過在動(dòng)物體內(nèi)長期輸注亞高壓劑量的Norepinephrine 可引起心肌質(zhì)量和左心室壁厚度的增加。Norepinephrine 通過 α1 和 β腎上腺素能刺激激活 raf-1 激酶/MAP 激酶級聯(lián)，來自兩個(gè)受體的信號(hào)通路協(xié)同誘導(dǎo)心肌細(xì)胞肥大。
    具體造模方法
    Rat: Spragues-Dawley rats • adult (6 months old) • Male &bull.
    Administration: Each rat was continuously injected with 100 μg/kg/h or 200 μg/kg/h through an osmotic minipump.
    造模成功指標(biāo)
    分子變化：顯著增加左心室 Dnmt活性和Dnmt1、3a 和 3b 的表達(dá)。
    顯著增加左心室中胎兒基因 ANP、BNP 和 βMHC 的 mRNA 表達(dá)。
    顯著增加左心室 ROS 生成并以濃度依賴方式增加整體基因組 DNA 甲基化和左心室 PKCε 啟動(dòng)子區(qū)域 Egr-1 結(jié)合位點(diǎn)基因特異性 CpG 甲基化。
    以濃度依賴性方式增加乳酸脫氫酶釋放。
    顯著降低左心室發(fā)育壓力和 dP/dtmax、誘導(dǎo)了肌營養(yǎng)蛋白的上調(diào)和四加半LIM域蛋白2 (FHL2) 的下調(diào)。
    表型觀測：增加心肌梗死面積并持續(xù)升高血壓。
    誘導(dǎo)心臟肥厚和心臟收縮力降低。
    增加左心室體重。
    拮抗產(chǎn)品 5-Aza-2’-deoxycytidine (HY-A0004), Prazosin (HY-B0193), Propranolol (HY-B0573B)

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    169.18

    Formula

    C8H11NO3
    CAS 號(hào)
    性狀

    固體

    顏色

    White to yellow

    中文名稱

    去甲腎上腺素
    結(jié)構(gòu)分類
    初始來源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : 50 mg/mL (295.54 mM; 超聲助溶; 酸性條件溶解 (HCL 調(diào)節(jié)，pH≈2); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    H2O 中的溶解度 : 33.33 mg/mL (197.01 mM; ultrasonic and adjust pH to 1 with 1 M HCL)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 5.9109 mL 29.5543 mL 59.1086 mL
    5 mM 1.1822 mL 5.9109 mL 11.8217 mL
    查看完整儲(chǔ)備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

    * 備注：如您選擇水作為儲(chǔ)備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    請根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (12.29 mM); 澄清溶液

      此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (12.29 mM); 澄清溶液

      此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
    動(dòng)物溶解方案計(jì)算器
    請輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請具體參考實(shí)測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: 99.43%

    參考文獻(xiàn)
    Cell Assay
    [2]

    Subcutaneous preadipocytes derived from a 38-year old non-diabetic female donor are immortalized with TERT and HPV E6/E7. For the current studies, a stable diploid clone (referred to as clone B) with consistent differentiation capacity is isolated by ring cloning. Cells are grown in preadipocyte PGM2 media. Once cells are confluent, differentiation is induced by incubation in differentiation media consisting of dexamethasone, IBMX, indomethacin, and additional insulin. Cells are differentiated for 10 days. Prior to treatment, media is replaced with PGM2 media for one day and then switched to serum-free media overnight for treatments. Adipocytes are treated for 6 hours with vehicle, Norepinephrine (NE, 10 μM), CGP (10 nM), or Norepinephrine (NE) and CGP[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.9109 mL 29.5543 mL 59.1086 mL 147.7716 mL
    5 mM 1.1822 mL 5.9109 mL 11.8217 mL 29.5543 mL
    10 mM 0.5911 mL 2.9554 mL 5.9109 mL 14.7772 mL
    15 mM 0.3941 mL 1.9703 mL 3.9406 mL 9.8514 mL
    20 mM 0.2955 mL 1.4777 mL 2.9554 mL 7.3886 mL
    25 mM 0.2364 mL 1.1822 mL 2.3643 mL 5.9109 mL
    30 mM 0.1970 mL 0.9851 mL 1.9703 mL 4.9257 mL
    40 mM 0.1478 mL 0.7389 mL 1.4777 mL 3.6943 mL
    50 mM 0.1182 mL 0.5911 mL 1.1822 mL 2.9554 mL
    60 mM 0.0985 mL 0.4926 mL 0.9851 mL 2.4629 mL
    80 mM 0.0739 mL 0.3694 mL 0.7389 mL 1.8471 mL
    100 mM 0.0591 mL 0.2955 mL 0.5911 mL 1.4777 mL

    * 備注：如您選擇水作為儲(chǔ)備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

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    產(chǎn)品名稱：
    Norepinephrine
    目錄號(hào)：
    HY-13715
    需求量：