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  2. Apoptosis Epigenetics Cell Cycle/DNA Damage Others
  3. Bcl-2 Family Caspase PARP Apoptosis Fluorescent Dye
  4. Chlorin e6

Chlorin e6  (Synonyms: Ce6)

目錄號: HY-13594 純度: 98.31%
COA 產品使用指南

Chlorin e6 是一種光敏劑，在波長 402 和 662 nm 處有較強的吸收峰，在 668 nm 處表現(xiàn)出強烈的熒光。Chlorin e6 具有抗菌功效和抗癌活性。Chlorin e6 通過激活 caspase-3 誘導細胞凋亡，可用于癌癥的研究。

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Chlorin e6 Chemical Structure

Chlorin e6 Chemical Structure

CAS No. : 19660-77-6

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規(guī)格 價格 是否有貨 數量
10 mM * 1 mL in DMSO ￥880
In-stock
50 mg ￥800
In-stock
100 mg ￥1300
In-stock
200 mg   詢價  
500 mg   詢價  

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Customer Review

  • 生物活性

  • 純度 & 產品資料

  • 參考文獻

生物活性

Chlorin e6 is a photosensitizer and has strong absorption peaks at wavelength of 402 and 662 nm, as well as exhibiting intense fluorescence at 668 nm. Chlorin e6 has antimicrobial efficacy and anticancer activity. Chlorin e6 induces cell apoptosis via caspase-3 activation and can be used for the research of cancer[1][2][3].

IC50 & Target

Leishmania

 

Bax

 

Bcl-2

 

Caspase 3

 

PARP-1

 

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
HeLa IC50
> 50 μM
Compound: Ce6
Dark toxicity in human HeLa cells assessed as reduction in cell viability after 3 hrs by MTT assay
Dark toxicity in human HeLa cells assessed as reduction in cell viability after 3 hrs by MTT assay
[PMID: 32787080]
HeLa IC50
1.92 μM
Compound: Ce6
Phototoxicity against human HeLa cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay
Phototoxicity against human HeLa cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay
[PMID: 32787080]
HEp-2 IC50
> 400 μM
Compound: 5
Cytotoxicity against human Hep2 cells under dark condition after 18 hrs by celltiter blue assay
Cytotoxicity against human Hep2 cells under dark condition after 18 hrs by celltiter blue assay
[PMID: 21936519]
HEp-2 IC50
20.8 μM
Compound: 5
Cytotoxicity against human Hep2 cells after overnight incubation followed by cells irradiated with 1 J/cm'2 light for 20 mins measured after overnight incubation by celltiter blue assay
Cytotoxicity against human Hep2 cells after overnight incubation followed by cells irradiated with 1 J/cm'2 light for 20 mins measured after overnight incubation by celltiter blue assay
[PMID: 21936519]
HepG2 IC50
41.2 μM
Compound: Chlorin e6
Photo cytotoxic activity against human HepG2 cells irradiated with 1.7 J/cm'2 light dose for 10 mins measured after overnight incubation by MTT assay
Photo cytotoxic activity against human HepG2 cells irradiated with 1.7 J/cm'2 light dose for 10 mins measured after overnight incubation by MTT assay
[PMID: 26306981]
HepG2 IC50
52.9 μM
Compound: Chlorin e6
Dark toxicity in human HepG2 cells after overnight incubation by MTT assay
Dark toxicity in human HepG2 cells after overnight incubation by MTT assay
[PMID: 26306981]
MCF7 IC50
> 50 μM
Compound: Ce6
Dark toxicity in human MCF7 cells assessed as reduction in cell viability after 3 hrs by MTT assay
Dark toxicity in human MCF7 cells assessed as reduction in cell viability after 3 hrs by MTT assay
[PMID: 32787080]
MCF7 IC50
3.4 μM
Compound: Ce6
Phototoxicity against human MCF7 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay
Phototoxicity against human MCF7 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay
[PMID: 32787080]
SK-OV-3 IC50
0.39 μM
Compound: Chlorine E6
Photocytotoxicity in human SKOV3 cells assessed as cell death incubated for 6 hrs followed by PDT-1200 light irradiation at 20 J/cm2 measured after 16 hrs using liposome-encapsulated compound by MTT assay
Photocytotoxicity in human SKOV3 cells assessed as cell death incubated for 6 hrs followed by PDT-1200 light irradiation at 20 J/cm2 measured after 16 hrs using liposome-encapsulated compound by MTT assay
[PMID: 29670694]
SW480 IC50
> 50 μM
Compound: Ce6
Dark toxicity in human SW480 cells assessed as reduction in cell viability after 3 hrs by MTT assay
Dark toxicity in human SW480 cells assessed as reduction in cell viability after 3 hrs by MTT assay
[PMID: 32787080]
SW480 IC50
3.1 μM
Compound: Ce6
Phototoxicity against human SW480 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay
Phototoxicity against human SW480 cells assessed as reduction in cell viability preincubated for 3 hrs followed by 54 J/cm2 light irradiation and measured after 24 hrs by MTT assay
[PMID: 32787080]
體外研究
(In Vitro)

Chlorin e6 (100 μg/mL, 24 h) 在 635 nm 激光照射下對 HT-29 細胞中沒有毒性[2]。
Chlorin e6 (5-10 μM, 3 h) 在 660 nm 近紅外激光照射下通過激活 caspase-3 誘導胰腺癌細胞凋亡，從而抑制 AsPC-1 和 MIA PaCa-2 細胞的腫瘤生長[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[3]

Cell Line: AsPC-1 and MIA PaCa-2 Cells
Concentration: 5 μM, 10 μM
Incubation Time: 3 h
Result: Significantly reduced Bcl-2 protein expression while increasing apoptotic molecule expression of Bax.
Activated caspase-3 through its cleavage and upregulated the expression of cleaved PARP-1 in a dose-dependent manner.
體內研究
(In Vivo)

Chlorin e6 (2.5 mg/kg，靜脈注射，單次劑量) 以 100 J/cm2 的紅光照射 8 min 20 s 在 B16F10 黑色素瘤細胞和 PANC02 胰腺細胞異種移植小鼠模型中具有抗腫瘤活性[3]。
Chlorin e6 (2.5 mg/kg，靜脈注射，單次劑量) 在 660 nm 近紅外激光照射下可抑制犬腫瘤生長[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B16F10 melanoma cells xenograft mouse, PANC02 pancreatic cells xenograft mouse[3]
Dosage: 2.5 mg/kg
Administration: Intravenous injection (i.v.)
Result: Significantly reduced tumor volume.
分子量

596.67

Formula

C34H36N4O6
CAS 號
性狀

固體

顏色

Brown to black

運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數據
細胞實驗： 

DMSO 中的溶解度 : 20 mg/mL (33.52 mM; 超聲助溶; 吸濕的 DMSO 對產品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質量 1 mg 5 mg 10 mg
1 mM 1.6760 mL 8.3798 mL 16.7597 mL
5 mM 0.3352 mL 1.6760 mL 3.3519 mL
查看完整儲備液配制表

* 請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內使用，-20°C儲存時，請在1個月內使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據您的 實驗動物和給藥方式 選擇適當的溶解方案。

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結果的可靠性，澄清的儲備液可以根據儲存條件，適當保存；體內實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (3.49 mM); 懸濁液; 超聲助溶

    此方案可獲得 2.08 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網站選購。 ，Tween 80，均可在 MCE 網站選購。
計算結果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的儲備液濃度超過該產品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術支持聯(lián)系。
動物實驗體內工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產品資料

純度: 98.31%

參考文獻

完整儲備液配制表

* 請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內使用，-20°C儲存時，請在1個月內使用。

可選溶劑 濃度 溶劑體積 質量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6760 mL 8.3798 mL 16.7597 mL 41.8992 mL
5 mM 0.3352 mL 1.6760 mL 3.3519 mL 8.3798 mL
10 mM 0.1676 mL 0.8380 mL 1.6760 mL 4.1899 mL
15 mM 0.1117 mL 0.5587 mL 1.1173 mL 2.7933 mL
20 mM 0.0838 mL 0.4190 mL 0.8380 mL 2.0950 mL
25 mM 0.0670 mL 0.3352 mL 0.6704 mL 1.6760 mL
30 mM 0.0559 mL 0.2793 mL 0.5587 mL 1.3966 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產品名稱：
Chlorin e6
目錄號：
HY-13594
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