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  2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related Anti-infection Apoptosis
  3. DNA Alkylator/Crosslinker DNA/RNA Synthesis ADC Cytotoxin Bacterial Apoptosis Antibiotic
  4. Mitomycin C

Mitomycin C  (Synonyms: 絲裂霉素 C ; Ametycine)

目錄號: HY-13316 純度: 99.98%
COA 產(chǎn)品使用指南

Mitomycin C (Ametycine) 是一種 DNA 交聯(lián)劑 (DNA cross-linking agent)，誘導(dǎo) DNA 損傷。Mitomycin C 是一種抗腫瘤化合物和抗生素，可有效的抑制 DNA 合成 (DNA synthesis)。Mitomycin C 是一種 ADC 毒性分子 (ADC Cytotoxin)，可以誘導(dǎo)凋亡 (Apoptosis) 作用。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Mitomycin C Chemical Structure

Mitomycin C Chemical Structure

CAS No. : 50-07-7

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MCE 顧客使用本產(chǎn)品發(fā)表的 111 篇科研文獻

Proliferation Assay

    Mitomycin C purchased from MCE. Usage Cited in: Sci Rep. 2017 Jun 30;7(1):4469.  [Abstract]

    In order to normalize the potential effect of cell proliferation, Mitomycin C is added to the culture media to make the cells uniformly arrested. The results show that all migration cell indexes reduced after adding Mitomycin C than those observed without Mitomycin C. Knockdown of RAD51-AS1 still inhibits cell migration (A) and invasion of SKOV3, SKOV3.ip and HO8910 cells (B), while overexpression of RAD51-AS1 only increased cell invasion capacity of the OVCAR3 cells, not in the Hey cells (C).

    Mitomycin C purchased from MCE. Usage Cited in: Exp Cell Res. 2016 Feb 15;341(2):157-65.  [Abstract]

    Curcumin inhibits the migration of BCPAP cells. Migration of cancer cells is assayed by wound-healing assay. Cells are cultured to nearly confluent cell monolayer and pretreated with Mitomycin C (10 μg/mL) for 2 h, followed by either DMSO or different dosages of curcumin for 6 h, before cells are wounded using yellow pipette tips. The cells are washed with PBS, and cultured for additional 24 h at 37 °C. (A) Photographs are taken after wound initially made and healing for 24 h. Data are represent

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Mitomycin C (Ametycine) is a DNA cross-linking agent and induces DNA damaging. Mitomycin C is an antitumor agent and antibiotic that shows extraordinary ability to inhibit DNA synthesis. Mitomycin C is an ADC Cytotoxin and induces apoptosis[1][2][3].

    IC50 & Target

    Traditional Cytotoxic Agents

     

    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    786-0 GI50
    0.21 μM
    Compound: Mitomycin
    Growth inhibition of human 786-O cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human 786-O cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    A2780 IC50
    0.18 μM
    Compound: MMC; MCC
    Antiproliferative activity against human A2780 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Antiproliferative activity against human A2780 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 33581556]
    A549 IC50
    0.04 μM
    Compound: Mitomycin-C
    Cytotoxicity against human A549 cells after 48 hrs by SRB assay
    Cytotoxicity against human A549 cells after 48 hrs by SRB assay
    		[PMID: 25259515]
    A549 GI50
    0.08 μM
    Compound: Mitomycin
    Growth inhibition of human A549 cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human A549 cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    A549 IC50
    0.24 μM
    Compound: MMC, mitomycin c
    Cytotoxicity against human A549 cells after 72 hrs
    Cytotoxicity against human A549 cells after 72 hrs
    		[PMID: 18037301]
    A549 IC50
    0.27 μM
    Compound: Mitomycin
    Cytotoxicity against human A549 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    		[PMID: 31490685]
    A549 IC50
    0.3 μM
    Compound: Mitomycin C
    Cytotoxicity against human A549 cells after 3 days by SRB assay
    Cytotoxicity against human A549 cells after 3 days by SRB assay
    		[PMID: 16038545]
    A549 IC50
    0.4 μM
    Compound: MMC
    Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
    Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
    		[PMID: 23123017]
    A549 IC50
    0.4 μM
    Compound: mitomycin C
    Cytotoxicity against human A549 cells after 72 hrs by Alamar Blue assay
    Cytotoxicity against human A549 cells after 72 hrs by Alamar Blue assay
    		[PMID: 18752870]
    A549 IC50
    0.4 μM
    Compound: MMC, mitomycin c
    Cytotoxicity against human A549 cells by Alamar blue assay
    Cytotoxicity against human A549 cells by Alamar blue assay
    		[PMID: 18037301]
    A549 IC50
    0.4 μM
    Compound: Mitomycin-C
    Cytotoxicity against Homo sapiens (human) A549 cells after 48 hr by SRB assay
    Cytotoxicity against Homo sapiens (human) A549 cells after 48 hr by SRB assay
    		10.1007/s00044-012-0424-0
    A549 IC50
    0.4 μM
    Compound: Mitomycin-C
    Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation after 48 hrs by sulforhodamine-B assay
    Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation after 48 hrs by sulforhodamine-B assay
    		10.1039/C3MD00055A
    A549 EC50
    0.43 μM
    Compound: mitomycin
    Antiproliferative activity against human A549 cells after 72 hrs by trypan blue exclusion assay
    Antiproliferative activity against human A549 cells after 72 hrs by trypan blue exclusion assay
    		[PMID: 19674905]
    A549 EC50
    0.43 μM
    Compound: Mitomycin
    Cytotoxicity against human A549 cells assessed as cell viability by tryptan blue staining method
    Cytotoxicity against human A549 cells assessed as cell viability by tryptan blue staining method
    		[PMID: 17950604]
    A549 IC50
    0.45 μM
    Compound: Mitomycin C
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 hrs by WST8 assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 hrs by WST8 assay
    		[PMID: 25442306]
    A549 IC50
    0.54 μg/mL
    Compound: Mitomycin
    Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay
    Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay
    		[PMID: 23451797]
    A549 IC50
    0.63 μM
    Compound: MMC; MCC
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 33581556]
    A549 IC50
    1.7 μM
    Compound: Mitomycin
    Cytotoxicity against human A549 cells after 72 hrs by CCK-8 assay
    Cytotoxicity against human A549 cells after 72 hrs by CCK-8 assay
    		[PMID: 26615890]
    A549 IC50
    11.03 μM
    Compound: Mitomycin C
    Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    		[PMID: 31761382]
    A549 IC50
    15.525 μM
    Compound: 1
    Antiproliferative activity against human A549 cells expressing NQO1 after 72 hrs by MTT assay
    Antiproliferative activity against human A549 cells expressing NQO1 after 72 hrs by MTT assay
    		[PMID: 28395199]
    A549 IC50
    2.75 μM
    Compound: Mitomycin C
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 34847663]
    A549 IC50
    3 μM
    Compound: mitomycin
    Cytotoxicity against human A549 cells by MTT assay
    Cytotoxicity against human A549 cells by MTT assay
    		[PMID: 22545792]
    A549 IC50
    4.5 μM
    Compound: MMC, mitomycin c
    Cytotoxicity against human A549 cells after 24 hrs
    Cytotoxicity against human A549 cells after 24 hrs
    		[PMID: 18037301]
    A549 EC50
    5.2 μM
    Compound: MMC
    Cytotoxicity against human A549 cells assessed as decrease in cell viability at 10 to 100 uM after 48 hrs by SRB assay
    Cytotoxicity against human A549 cells assessed as decrease in cell viability at 10 to 100 uM after 48 hrs by SRB assay
    		[PMID: 26508550]
    Bcap37 IC50
    2.33 μg/mL
    Compound: Mitomycin
    Antitumor activity against human Bcap37 cell line by MTT assay
    Antitumor activity against human Bcap37 cell line by MTT assay
    		[PMID: 16455245]
    BeWo IC50
    0.06 μM
    Compound: Mitomycin
    Antiproliferative activity against human BeWo cells assessed as reduction in cell viability after 72 hrs by MTT assay
    Antiproliferative activity against human BeWo cells assessed as reduction in cell viability after 72 hrs by MTT assay
    		[PMID: 27010926]
    BeWo IC50
    0.85 μg/mL
    Compound: (21) mitomycin
    In vitro cytotoxicity of compound against Bewo, human choriocarcinoma was defined by microculture tetrazolium assay
    In vitro cytotoxicity of compound against Bewo, human choriocarcinoma was defined by microculture tetrazolium assay
    		[PMID: 12620075]
    BXPC-3 GI50
    0.38 μM
    Compound: Mitomycin
    Growth inhibition of human BxPC3 cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human BxPC3 cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    Caco-2 IC50
    5.8 μM
    Compound: Mitomycin
    Cytotoxicity against human Caco2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human Caco2 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    		[PMID: 31490685]
    Ca-Ski IC50
    > 10 μM
    Compound: Mitomycin C
    Antiproliferative activity against human CaSki cells after 48 hrs by MTT assay
    Antiproliferative activity against human CaSki cells after 48 hrs by MTT assay
    		[PMID: 29280632]
    CCRF S-180 ED50
    4.4 μg/mL
    Compound: 30
    Antitumor activity against mouse S180 cells implanted in ip dosed ddY mouse assessed as tumor volume after 7 days
    Antitumor activity against mouse S180 cells implanted in ip dosed ddY mouse assessed as tumor volume after 7 days
    		[PMID: 19540628]
    CHO-AA8 IC50
    1.08 nM
    Compound: Mitomycin C
    Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours)
    Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours)
    		[PMID: 2909741]
    CHO-K1 IC50
    13.1 μg/mL
    Compound: Mitomycin C
    Cytotoxicity against CHOK1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against CHOK1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    		[PMID: 31195172]
    CHO-K1 IC50
    13.2 μM
    Compound: Mitomycin c
    Cytotoxicity against CHOK1 cells incubated for 24 hrs by MTT asasy
    Cytotoxicity against CHOK1 cells incubated for 24 hrs by MTT asasy
    		[PMID: 28615134]
    CHO-K1 IC50
    39.8 μM
    Compound: Mitomycin C
    Cytotoxicity against CHOK1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
    Cytotoxicity against CHOK1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
    		[PMID: 31858800]
    CNE-2 IC50
    > 10 μM
    Compound: Mitomycin C
    Antiproliferative activity against human CNE2 cells after 48 hrs by MTT assay
    Antiproliferative activity against human CNE2 cells after 48 hrs by MTT assay
    		[PMID: 29280632]
    Daoy IC50
    0.05 μg/mL
    Compound: Mitomycin
    Cytotoxicity against human DaOY cells after 1 to 11 days by MTT assay
    Cytotoxicity against human DaOY cells after 1 to 11 days by MTT assay
    		[PMID: 22014827]
    Daoy ED50
    0.08 μg/mL
    Compound: Mitomycin-C
    Antiproliferative activity against human DaOY cells after 6 days by MTT assay
    Antiproliferative activity against human DaOY cells after 6 days by MTT assay
    		[PMID: 22672798]
    Daoy ED50
    0.1 μg/mL
    Compound: mitomycin C
    Cytotoxicity against human DaOY cells after 3 days by MTT assay
    Cytotoxicity against human DaOY cells after 3 days by MTT assay
    		[PMID: 21115251]
    Daoy ED50
    0.14 μg/mL
    Compound: Mitomycin C
    Cytotoxicity against human DaOY cells by MTT assay
    Cytotoxicity against human DaOY cells by MTT assay
    		[PMID: 20144547]
    Daoy ED50
    0.21 μM
    Compound: mitomycin C
    Cytotoxicity against human Daoy cells by MTT assay
    Cytotoxicity against human Daoy cells by MTT assay
    		[PMID: 19061391]
    Daudi IC50
    0.45 μM
    Compound: Mitomycin C
    Growth inhibition of human Daudi cells after 24 to 72 hrs by MTT assay
    Growth inhibition of human Daudi cells after 24 to 72 hrs by MTT assay
    		[PMID: 20117936]
    DLD-1 ED50
    0.1 μg/mL
    Compound: mitomycin
    Cytotoxicity against human DLD1 cells after 3 days by methylene blue staining
    Cytotoxicity against human DLD1 cells after 3 days by methylene blue staining
    		[PMID: 16309320]
    DU-145 IC50
    0.17 μM
    Compound: MMC, NSC-26980
    Cytotoxicity against human DU145 cells assessed as reduction in mean cell viability after 72 hrs by MTT assay
    Cytotoxicity against human DU145 cells assessed as reduction in mean cell viability after 72 hrs by MTT assay
    		[PMID: 22522008]
    DU-145 IC50
    0.17 μM
    Compound: MMC
    Cytotoxicity against human DU145 cells after 72 hrs by MTT assay
    Cytotoxicity against human DU145 cells after 72 hrs by MTT assay
    		[PMID: 20605274]
    DU-145 IC50
    1.8 μM
    Compound: Mitomycin C
    Cytotoxicity against Homo sapiens (human) DU145 cells by SRB assay
    Cytotoxicity against Homo sapiens (human) DU145 cells by SRB assay
    		10.1007/s00044-010-9500-5
    EL4 IC50
    0.06 μM
    Compound: Mitomycin
    Antiproliferative activity against mouse EL4 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
    Antiproliferative activity against mouse EL4 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
    		[PMID: 27010926]
    EL4 IC50
    3.94 μg/mL
    Compound: (21) mitomycin
    In vitro cytotoxicity of compound against EL4, mouse thymoma was defined by microculture tetrazolium assay
    In vitro cytotoxicity of compound against EL4, mouse thymoma was defined by microculture tetrazolium assay
    		[PMID: 12620075]
    EMT6 ED50
    0.001 μM
    Compound: mytomycin C
    Compound is measured for surviving fractions of EMT6 tumor cells treated in culture with (0.50 uM) concentration for 1 hr under hypoxic condition.
    Compound is measured for surviving fractions of EMT6 tumor cells treated in culture with (0.50 uM) concentration for 1 hr under hypoxic condition.
    		[PMID: 7452674]
    EMT6 ED50
    0.7 μM
    Compound: mytomycin C
    Compound is measured for surviving fractions of EMT6 tumor cells treated in culture with (0.50 uM) concentration for 1 hr under aerobic condition.
    Compound is measured for surviving fractions of EMT6 tumor cells treated in culture with (0.50 uM) concentration for 1 hr under aerobic condition.
    		[PMID: 7452674]
    Fibroblast EC50
    0.93 μM
    Compound: mitomycin
    Antiproliferative activity against human skin fibroblast after 72 hrs by trypan blue exclusion assay
    Antiproliferative activity against human skin fibroblast after 72 hrs by trypan blue exclusion assay
    		[PMID: 19674905]
    GES1 IC50
    21.12 μM
    Compound: Mitomycin C
    Cytotoxicity against human GES1 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human GES1 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    		[PMID: 31761382]
    HCT-116 GI50
    0.2 μM
    Compound: Mitomycin
    Growth inhibition of human HCT116 cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human HCT116 cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    HCT-116 IC50
    0.5 μM
    Compound: Mytomycine
    Antiproliferative activity against human HCT116 cells after 48 hrs by sulforhodamine B assay
    Antiproliferative activity against human HCT116 cells after 48 hrs by sulforhodamine B assay
    		[PMID: 23501113]
    HCT-116 IC50
    0.55 μM
    Compound: Mitomycin C
    Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 34847663]
    HCT-116 IC50
    0.56 μM
    Compound: MMC; MCC
    Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 33581556]
    HCT-116 IC50
    11.03 μM
    Compound: Mitomycin C
    Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    		[PMID: 31761382]
    HCT-15 IC50
    0.07 μM
    Compound: Mitomycin C
    In vitro cytotoxic activity against human colon cancer cell line HCT15 after 72 hr of drug exposure determined by the SRB assay
    In vitro cytotoxic activity against human colon cancer cell line HCT15 after 72 hr of drug exposure determined by the SRB assay
    		[PMID: 14980672]
    HCT-15 IC50
    0.5 μM
    Compound: Mitomycin
    Cytotoxicity against human HCT15 cells after 48 hrs by sulforhodamine B assay
    Cytotoxicity against human HCT15 cells after 48 hrs by sulforhodamine B assay
    		[PMID: 24910974]
    HCT-8 IC50
    0.083 μM
    Compound: MMC
    Cytotoxicity against human HCT8 cells after 72 hrs by SRB assay
    Cytotoxicity against human HCT8 cells after 72 hrs by SRB assay
    		[PMID: 18845441]
    HCT-8 IC50
    0.6 μM
    Compound: Mitomycin C
    Cytotoxicity against human HCT8 cells after 3 days by SRB assay
    Cytotoxicity against human HCT8 cells after 3 days by SRB assay
    		[PMID: 16038545]
    HeLa IC50
    0.01 μg/mL
    Compound: Mytomycin C
    Cytotoxicity against human HeLa cells after 3 days by methylene blue assay
    Cytotoxicity against human HeLa cells after 3 days by methylene blue assay
    		[PMID: 9644081]
    HeLa IC50
    0.06 μg/mL
    Compound: Mitomycin
    Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay
    Cytotoxicity against human HeLa cells after 1 to 11 days by MTT assay
    		[PMID: 22014827]
    HeLa ED50
    0.13 μg/mL
    Compound: Mitomycin-C
    Antiproliferative activity against human HeLa cells after 6 days by MTT assay
    Antiproliferative activity against human HeLa cells after 6 days by MTT assay
    		[PMID: 22672798]
    HeLa ED50
    0.2 μg/mL
    Compound: mitomycin C
    Cytotoxicity against human HeLa cells after 3 days by MTT assay
    Cytotoxicity against human HeLa cells after 3 days by MTT assay
    		[PMID: 21115251]
    HeLa IC50
    0.2 μM
    Compound: MMC; MCC
    Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 33581556]
    HeLa IC50
    0.27 μM
    Compound: MMC, NSC-26980
    Cytotoxicity against human HeLa cells assessed as reduction in mean cell viability after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells assessed as reduction in mean cell viability after 72 hrs by MTT assay
    		[PMID: 22522008]
    HeLa IC50
    0.27 μM
    Compound: MMC
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    		[PMID: 20605274]
    HeLa IC50
    1.13 μM
    Compound: MMC
    Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay
    Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay
    		[PMID: 29475587]
    HeLa IC50
    1870 nM
    Compound: MC
    Antiproliferative activity against human HeLa cells after 48 hrs by resazurin reduction assay
    Antiproliferative activity against human HeLa cells after 48 hrs by resazurin reduction assay
    		[PMID: 26541587]
    HeLa IC50
    2 μM
    Compound: Mitomycin-C
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    		[PMID: 25176329]
    HeLa IC50
    2 μM
    Compound: Mitomycin-c
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    		[PMID: 24056146]
    HeLa IC50
    3 μM
    Compound: Mitomycin
    Cytotoxicity against human HeLa cells after 48 hrs by SRB method
    Cytotoxicity against human HeLa cells after 48 hrs by SRB method
    		[PMID: 24913558]
    HeLa IC50
    3.3 μM
    Compound: mitomycin
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    		[PMID: 19432411]
    HeLa IC50
    3.4 μM
    Compound: mitomycin C
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    Cytotoxicity against human HeLa cells after 48 hrs by SRB assay
    		[PMID: 22642381]
    HeLa IC50
    68 nM
    Compound: mitomycin C
    Cytotoxicity against human HeLa cells assessed as inhibition of colony formation after 5 to 8 days by colony assay
    Cytotoxicity against human HeLa cells assessed as inhibition of colony formation after 5 to 8 days by colony assay
    		[PMID: 11975488]
    HeLa S3 IC50
    0.52 μM
    Compound: Mitomycin C
    Cytotoxicity against human HeLa S3 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Cytotoxicity against human HeLa S3 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 35766102]
    Hep 3B2 IC50
    0.01 μg/mL
    Compound: Mytomycin C
    Cytotoxicity against human Hep3B cells after 3 days by methylene blue assay
    Cytotoxicity against human Hep3B cells after 3 days by methylene blue assay
    		[PMID: 9644081]
    HEp-2 ED50
    0.07 μg/mL
    Compound: Mitomycin C
    Cytotoxicity against human Hep2 cells by MTT assay
    Cytotoxicity against human Hep2 cells by MTT assay
    		[PMID: 20144547]
    HEp-2 ED50
    0.1 μg/mL
    Compound: mitomycin C
    Cytotoxicity against human Hep2 cells after 3 days by MTT assay
    Cytotoxicity against human Hep2 cells after 3 days by MTT assay
    		[PMID: 21115251]
    HEp-2 ED50
    0.12 μg/mL
    Compound: Mitomycin-C
    Antiproliferative activity against human Hep2 cells after 6 days by MTT assay
    Antiproliferative activity against human Hep2 cells after 6 days by MTT assay
    		[PMID: 22672798]
    HEp-2 IC50
    1.5 μM
    Compound: Mto-C
    Growth inhibition of human Hep2 cells after 48 hrs by sulforhodamine B assay
    Growth inhibition of human Hep2 cells after 48 hrs by sulforhodamine B assay
    		[PMID: 19939522]
    Hepatocyte EC50
    1.46 μM
    Compound: mitomycin
    Antiproliferative activity against human hepatocytes after 72 hrs by trypan blue exclusion assay
    Antiproliferative activity against human hepatocytes after 72 hrs by trypan blue exclusion assay
    		[PMID: 19674905]
    HepG2 IC50
    > 10 μM
    Compound: Mitomycin C
    Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
    Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 29280632]
    HepG2 IC50
    0.13 μM
    Compound: Mitomycin
    Anticancer activity against human HepG2 cells by MTT assay
    Anticancer activity against human HepG2 cells by MTT assay
    		[PMID: 34128674]
    HepG2 IC50
    0.14 μg/mL
    Compound: Mitomycin
    Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay
    Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay
    		[PMID: 23451797]
    HepG2 IC50
    2.27 μM
    Compound: Mitomycin C
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 34847663]
    HepG2 IC50
    2937 nM
    Compound: MC
    Antiproliferative activity against human HepG2 cells after 48 hrs by resazurin reduction assay
    Antiproliferative activity against human HepG2 cells after 48 hrs by resazurin reduction assay
    		[PMID: 26541587]
    HepG2 IC50
    32.63 μM
    Compound: Mitomycin C
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    		[PMID: 31761382]
    HepG2 IC50
    5.4 μM
    Compound: Mitomycin
    Cytotoxicity against human HepG2 by MTT assay
    Cytotoxicity against human HepG2 by MTT assay
    		[PMID: 20968296]
    HL-60 IC50
    0.1 μM
    Compound: MMC
    Cytotoxicity against human HL60 cells after 72 hrs by WST8 assay
    Cytotoxicity against human HL60 cells after 72 hrs by WST8 assay
    		[PMID: 22537363]
    HL-60 IC50
    0.19 μM
    Compound: MMC
    Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
    Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
    		[PMID: 29475587]
    HL-60 IC50
    1.5 μM
    Compound: Mitomycin
    Cytotoxicity against human HL60 by MTT assay
    Cytotoxicity against human HL60 by MTT assay
    		[PMID: 20968296]
    HL-60 IC50
    1.5 μM
    Compound: mitomycin
    Cytotoxicity against human HL60 cells after 48 hrs by SRB assay
    Cytotoxicity against human HL60 cells after 48 hrs by SRB assay
    		[PMID: 19432411]
    HL-60 IC50
    1.8 μM
    Compound: mitomycin C
    Cytotoxicity against human HL60 cells after 48 hrs by SRB assay
    Cytotoxicity against human HL60 cells after 48 hrs by SRB assay
    		[PMID: 22642381]
    HL-60 IC50
    3 μM
    Compound: Mitomycin
    Cytotoxicity against human HL60 cells after 48 hrs by SRB method
    Cytotoxicity against human HL60 cells after 48 hrs by SRB method
    		[PMID: 24913558]
    HL-60 IC50
    410 nM
    Compound: MC
    Antiproliferative activity against human HL60 cells after 48 hrs by resazurin reduction assay
    Antiproliferative activity against human HL60 cells after 48 hrs by resazurin reduction assay
    		[PMID: 26541587]
    HT-1080 IC50
    0.15 μg/mL
    Compound: Mitomycin
    Antiproliferative activity against human HT1080 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay
    Antiproliferative activity against human HT1080 cells assessed as inhibition of cell proliferation after 72 hrs by CCK8 assay
    		[PMID: 23451797]
    HT-29 IC50
    0.02 μg/mL
    Compound: (21) mitomycin
    In vitro cytotoxicity of compound against HT29, human colorectal adenocarcinoma was defined by microculture tetrazolium assay
    In vitro cytotoxicity of compound against HT29, human colorectal adenocarcinoma was defined by microculture tetrazolium assay
    		[PMID: 12620075]
    HT-29 IC50
    0.06 μM
    Compound: Mitomycin
    Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 72 hrs by MTT assay
    Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 72 hrs by MTT assay
    		[PMID: 27010926]
    HT-29 IC50
    0.44 μM
    Compound: Mitomycin C
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 34847663]
    HT-29 IC50
    0.9 μM
    Compound: mitomycin C
    Cytotoxicity against human HT29 cells after 72 hrs by MTT assay
    Cytotoxicity against human HT29 cells after 72 hrs by MTT assay
    		[PMID: 18222567]
    HT-29 IC50
    2.1 μM
    Compound: mitomycin C
    Cytotoxicity against human HT29 cells after 72 hrs by MTT assay in presence of 2 uM dicoumarol
    Cytotoxicity against human HT29 cells after 72 hrs by MTT assay in presence of 2 uM dicoumarol
    		[PMID: 18222567]
    HT-29 IC50
    7.099 μM
    Compound: 1
    Antiproliferative activity against human HT-29 cells expressing NQO1 after 72 hrs by MTT assay
    Antiproliferative activity against human HT-29 cells expressing NQO1 after 72 hrs by MTT assay
    		[PMID: 28395199]
    HT-29 IC50
    99 nM
    Compound: Mitomycin C
    Antiproliferative activity against human HT-29 cells after 96 hrs by MTT assay
    Antiproliferative activity against human HT-29 cells after 96 hrs by MTT assay
    		[PMID: 2778449]
    Huh-7 GI50
    2 μM
    Compound: Mitomycin
    Growth inhibition of human HuH7 cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human HuH7 cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    HuT78 IC50
    66 nM
    Compound: MC
    Antiproliferative activity against human HUT78 cells after 48 hrs by resazurin reduction assay
    Antiproliferative activity against human HUT78 cells after 48 hrs by resazurin reduction assay
    		[PMID: 26541587]
    Jurkat IC50
    0.43 μM
    Compound: Mitomycin
    Cytotoxicity against human Jurkat cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human Jurkat cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    		[PMID: 31490685]
    Jurkat IC50
    303 nM
    Compound: MC
    Antiproliferative activity against human Jurkat T cells in hypoxic condition after 48 hrs by resazurin reduction assay
    Antiproliferative activity against human Jurkat T cells in hypoxic condition after 48 hrs by resazurin reduction assay
    		[PMID: 26541587]
    Jurkat IC50
    578 nM
    Compound: MC
    Antiproliferative activity against human Jurkat T cells after 48 hrs by resazurin reduction assay
    Antiproliferative activity against human Jurkat T cells after 48 hrs by resazurin reduction assay
    		[PMID: 26541587]
    K562 IC50
    0.47 μM
    Compound: Mitomycin C
    Growth inhibition of human K562 cells after 24 to 72 hrs by MTT assay
    Growth inhibition of human K562 cells after 24 to 72 hrs by MTT assay
    		[PMID: 20117936]
    K562 IC50
    3.35 μg/mL
    Compound: Mitomycin
    Antitumor activity against human K562 cell line by MTT assay
    Antitumor activity against human K562 cell line by MTT assay
    		[PMID: 16455245]
    K562-Lucena 1 IC50
    2.75 μM
    Compound: Mitomycin C
    Growth inhibition of human K562-Lucena 1 cells after 24 to 72 hrs by MTT assay
    Growth inhibition of human K562-Lucena 1 cells after 24 to 72 hrs by MTT assay
    		[PMID: 20117936]
    KB IC50
    0.01 μg/mL
    Compound: mitomycin C
    Cytotoxicity against human KB cells after 72 hrs by methylene blue assay
    Cytotoxicity against human KB cells after 72 hrs by methylene blue assay
    		[PMID: 12502326]
    KB IC50
    0.01 μg/mL
    Compound: mitomycin C
    Cytotoxicity against human KB cells after 72 hrs by methylene blue assay
    Cytotoxicity against human KB cells after 72 hrs by methylene blue assay
    		[PMID: 11473432]
    KB IC50
    0.08 μg/mL
    Compound: Mitomycin
    Cytotoxicity against human KB cells after 1 to 11 days by MTT assay
    Cytotoxicity against human KB cells after 1 to 11 days by MTT assay
    		[PMID: 22014827]
    KB ED50
    0.13 μg/mL
    Compound: mitomycin
    Cytotoxicity against human KB cells after 3 days by methylene blue staining
    Cytotoxicity against human KB cells after 3 days by methylene blue staining
    		[PMID: 16309320]
    KB IC50
    0.4 μM
    Compound: Mytomycin C
    In vitro cytotoxicity determined against human epidermoid carcinoma KB cells by MTT assay
    In vitro cytotoxicity determined against human epidermoid carcinoma KB cells by MTT assay
    		10.1016/S0960-894X(97)00100-5
    KB IC50
    0.4 μM
    Compound: Mitomycin C
    In vitro antitumor activity against human epidermoid carcinoma KB cells by MTT assay.
    In vitro antitumor activity against human epidermoid carcinoma KB cells by MTT assay.
    		10.1016/0960-894X(95)00353-U
    KB ED50
    0.56 μg/mL
    Compound: Mitomycin
    Cytotoxicity against human KB cells after 72 hrs
    Cytotoxicity against human KB cells after 72 hrs
    		[PMID: 16643051]
    KB IC50
    0.6 μM
    Compound: Mitomycin C
    Cytotoxicity against human KB cells after 3 days by SRB assay
    Cytotoxicity against human KB cells after 3 days by SRB assay
    		[PMID: 16038545]
    KB 3-1 IC50
    0.1 μg/mL
    Compound: Mitomycin C
    Cytotoxicity against human KB-3-1 cells after 72 hrs by MTT assay
    Cytotoxicity against human KB-3-1 cells after 72 hrs by MTT assay
    		[PMID: 20149652]
    L02 IC50
    25.023 μM
    Compound: 1
    Cytotoxicity against human LO2 cells after 72 hrs by MTT assay
    Cytotoxicity against human LO2 cells after 72 hrs by MTT assay
    		[PMID: 28395199]
    L1210 IC50
    0.6 μM
    Compound: Mytomycin C
    In vitro cytotoxicity determined against murine leukemia L1210 cells by MTT assay
    In vitro cytotoxicity determined against murine leukemia L1210 cells by MTT assay
    		10.1016/S0960-894X(97)00100-5
    L1210 IC50
    0.6 μM
    Compound: Mitomycin C
    In vitro antitumor activity against murine lymphoma L1210 cells by MTT assay.
    In vitro antitumor activity against murine lymphoma L1210 cells by MTT assay.
    		10.1016/0960-894X(95)00353-U
    L6 ED50
    20.7 μM
    Compound: mitomycin C
    Cytotoxicity against human SCL6 cells by MTT assay
    Cytotoxicity against human SCL6 cells by MTT assay
    		[PMID: 12880314]
    M14 IC50
    0.47 μM
    Compound: Mitomycin C
    Cytotoxicity against human M14 cells after 72 hrs by MTS assay
    Cytotoxicity against human M14 cells after 72 hrs by MTS assay
    		[PMID: 26335269]
    MCF7 IC50
    0.06 μM
    Compound: Mitomycin
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs by SRB assay
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs by SRB assay
    		[PMID: 27010926]
    MCF7 ED50
    0.09 μg/mL
    Compound: Mitomycin-C
    Antiproliferative activity against human MCF7 cells after 6 days by MTT assay
    Antiproliferative activity against human MCF7 cells after 6 days by MTT assay
    		[PMID: 22672798]
    MCF7 ED50
    0.1 μg/mL
    Compound: mitomycin C
    Cytotoxicity against human MCF7 cells after 3 days by MTT assay
    Cytotoxicity against human MCF7 cells after 3 days by MTT assay
    		[PMID: 21115251]
    MCF7 ED50
    0.14 μg/mL
    Compound: Mitomycin C
    Cytotoxicity against human MCF7 cells by MTT assay
    Cytotoxicity against human MCF7 cells by MTT assay
    		[PMID: 20144547]
    MCF7 IC50
    0.2 μM
    Compound: mitomycin C
    Cytotoxicity against human MCF7 cells after 72 hrs by Alamar Blue assay
    Cytotoxicity against human MCF7 cells after 72 hrs by Alamar Blue assay
    		[PMID: 18752870]
    MCF7 IC50
    0.2 μM
    Compound: MMC, mitomycin c
    Cytotoxicity against human MCF7 cells by Alamar blue assay
    Cytotoxicity against human MCF7 cells by Alamar blue assay
    		[PMID: 18037301]
    MCF7 IC50
    0.2 μM
    Compound: Mitomycin-C
    Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
    Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
    		[PMID: 25259515]
    MCF7 IC50
    0.93 μg/mL
    Compound: (21) mitomycin
    In vitro cytotoxicity of compound against MCF7, human breast cancer was defined by microculture tetrazolium assay
    In vitro cytotoxicity of compound against MCF7, human breast cancer was defined by microculture tetrazolium assay
    		[PMID: 12620075]
    MCF7 EC50
    0.96 μM
    Compound: mitomycin
    Antiproliferative activity against human MCF7 cells after 72 hrs by trypan blue exclusion assay
    Antiproliferative activity against human MCF7 cells after 72 hrs by trypan blue exclusion assay
    		[PMID: 19674905]
    MCF7 EC50
    0.96 μM
    Compound: Mitomycin
    Cytotoxicity against human MCF7 cells assessed as cell viability by tryptan blue staining method
    Cytotoxicity against human MCF7 cells assessed as cell viability by tryptan blue staining method
    		[PMID: 17950604]
    MCF7 IC50
    1.2 μM
    Compound: MMC, mitomycin c
    Cytotoxicity against human MCF7 cells after 72 hrs
    Cytotoxicity against human MCF7 cells after 72 hrs
    		[PMID: 18037301]
    MCF7 IC50
    1.5 μM
    Compound: Mitomycin C
    Cytotoxicity against human MCF7 cells after 3 days by SRB assay
    Cytotoxicity against human MCF7 cells after 3 days by SRB assay
    		[PMID: 16038545]
    MCF7 IC50
    1.8 μM
    Compound: Mitomycin C
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 35766102]
    MCF7 IC50
    16.71 μM
    Compound: Mitomycin C
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    		[PMID: 31761382]
    MCF7 GI50
    2.5 μM
    Compound: Mitomycin
    Growth inhibition of human MCF7 cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human MCF7 cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    MCF7 IC50
    5 μM
    Compound: MMC, mitomycin c
    Cytotoxicity against human MCF7 cells after 24 hrs
    Cytotoxicity against human MCF7 cells after 24 hrs
    		[PMID: 18037301]
    MDA-MB-231 EC50
    0.103 μM
    Compound: Mitomycin
    Cytotoxicity against human MDA-MB-231 cells overexpressing DT-diaphorase after 5 days
    Cytotoxicity against human MDA-MB-231 cells overexpressing DT-diaphorase after 5 days
    		[PMID: 24436995]
    MDA-MB-231 EC50
    1.25 μM
    Compound: Mitomycin
    Cytotoxicity against wild-type human MDA-MB-231 cells after 5 days
    Cytotoxicity against wild-type human MDA-MB-231 cells after 5 days
    		[PMID: 24436995]
    MDA-MB-231 IC50
    2.89 μM
    Compound: Mitomycin C
    Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 34847663]
    MDA-MB-231 IC50
    4.4 μM
    Compound: Mitomycin C
    Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay
    Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay
    		[PMID: 29280632]
    MDA-MB-468 IC50
    0.27 μM
    Compound: Mitomycin C
    In vitro cytotoxic activity against human breast cancer cell line MD-MB-468 after 72 hr of drug exposure determined by the SRB assay
    In vitro cytotoxic activity against human breast cancer cell line MD-MB-468 after 72 hr of drug exposure determined by the SRB assay
    		[PMID: 14980672]
    MDA-MB-468 IC50
    0.5 μM
    Compound: Mitomycin C
    Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTS assay
    Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTS assay
    		[PMID: 26335269]
    MKN-74 GI50
    1.7 μM
    Compound: Mitomycin
    Growth inhibition of human MKN74 cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human MKN74 cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    NCI-H226 IC50
    0.58 μM
    Compound: Mitomycin C
    Cytotoxicity against human NCI-H226 cells after 72 hrs by MTS assay
    Cytotoxicity against human NCI-H226 cells after 72 hrs by MTS assay
    		[PMID: 26335269]
    NCI-H23 IC50
    2 μM
    Compound: Mytomycin
    Cytotoxicity against human NCI-H23 cells after 48 hrs by MTT assay
    Cytotoxicity against human NCI-H23 cells after 48 hrs by MTT assay
    		[PMID: 23792352]
    NCI-H596 IC50
    11.969 μM
    Compound: 1
    Antiproliferative activity against NQO1 deficient human NCI-H596 cells after 72 hrs by MTT assay
    Antiproliferative activity against NQO1 deficient human NCI-H596 cells after 72 hrs by MTT assay
    		[PMID: 28395199]
    NIH3T3 IC50
    1.7 μM
    Compound: 1, Mitomycin C (MMC)
    Inhibition of NIH 3T3 cell growth required to induce 50% of maximum response using antiproliferative assay
    Inhibition of NIH 3T3 cell growth required to induce 50% of maximum response using antiproliferative assay
    		[PMID: 11855992]
    NUGC-4 ED50
    19.1 μM
    Compound: mitomycin C
    Cytotoxicity against human NUGC4 cells by MTT assay
    Cytotoxicity against human NUGC4 cells by MTT assay
    		[PMID: 12880314]
    P388 IC50
    0.02 μg/mL
    Compound: mitomycin C
    Cytotoxicity against mouse P388 cells after 72 hrs by MTT assay
    Cytotoxicity against mouse P388 cells after 72 hrs by MTT assay
    		[PMID: 8254344]
    P388 IC50
    0.06 μM
    Compound: Mitomycin
    Antiproliferative activity against mouse P388 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
    Antiproliferative activity against mouse P388 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay
    		[PMID: 27010926]
    P388 IC50
    0.15 μg/mL
    Compound: Mitomycin C
    Cytotoxicity against mouse P388 cells by MTT assay
    Cytotoxicity against mouse P388 cells by MTT assay
    		[PMID: 17576066]
    P388 IC50
    1.5 μg/mL
    Compound: (21) mitomycin
    In vitro cytotoxicity of compound against P388, mouse fibroblast leukemia was defined by microculture tetrazolium assay
    In vitro cytotoxicity of compound against P388, mouse fibroblast leukemia was defined by microculture tetrazolium assay
    		[PMID: 12620075]
    P388 IC50
    3000 nM
    Compound: Mitomycin C
    Antiproliferative activity against mouse P388 cells after 48 hrs by MTT assay
    Antiproliferative activity against mouse P388 cells after 48 hrs by MTT assay
    		[PMID: 2778449]
    PBMC IC50
    4.03 μM
    Compound: Mitomycin C
    Growth inhibition of phytohemagglutinin-activated human PBMC cells after 24 to 72 hrs by MTT assay
    Growth inhibition of phytohemagglutinin-activated human PBMC cells after 24 to 72 hrs by MTT assay
    		[PMID: 20117936]
    PC-3 EC50
    0.14 μM
    Compound: mitomycin
    Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue exclusion assay
    Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue exclusion assay
    		[PMID: 19674905]
    PC-3 EC50
    0.14 μM
    Compound: Mitomycin
    Cytotoxicity against human PC3 cells assessed as cell viability by tryptan blue staining method
    Cytotoxicity against human PC3 cells assessed as cell viability by tryptan blue staining method
    		[PMID: 17950604]
    PC-3 IC50
    0.2 μM
    Compound: Mitomycin-C
    Cytotoxicity against human PC3 cells assessed as inhibition of cell growth after 48 hrs by sulforhodamine B assay
    Cytotoxicity against human PC3 cells assessed as inhibition of cell growth after 48 hrs by sulforhodamine B assay
    		10.1039/C5MD00289C
    PC-3 IC50
    1.4 μM
    Compound: Mitomycin
    Cytotoxicity against human PC3 cells after 72 hrs by CCK-8 assay
    Cytotoxicity against human PC3 cells after 72 hrs by CCK-8 assay
    		[PMID: 26615890]
    PC-3 IC50
    1.4 μM
    Compound: Mitomycin C
    Cytotoxicity against Homo sapiens (human) PC3 cells by SRB assay
    Cytotoxicity against Homo sapiens (human) PC3 cells by SRB assay
    		10.1007/s00044-010-9500-5
    PC-3 IC50
    1.5 μM
    Compound: Mitomycin-C
    Cytotoxicity against Homo sapiens (human) PC3 cells after 48 hr by SRB assay
    Cytotoxicity against Homo sapiens (human) PC3 cells after 48 hr by SRB assay
    		10.1007/s00044-012-0424-0
    PC-3 IC50
    1.5 μM
    Compound: Mitomycin-C
    Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
    Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
    		[PMID: 25176329]
    PC-3 IC50
    1.5 μM
    Compound: Mitomycin-c
    Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
    Cytotoxicity against human PC3 cells after 48 hrs by SRB assay
    		[PMID: 24056146]
    PC-3 IC50
    1.5 μM
    Compound: Mitomycin-C
    Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation after 48 hrs by sulforhodamine-B assay
    Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation after 48 hrs by sulforhodamine-B assay
    		10.1039/C3MD00055A
    PC-3 IC50
    1.5 μM
    Compound: Mto-C
    Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay
    Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay
    		[PMID: 19939522]
    RKO IC50
    1.75 μg/mL
    Compound: Mitomycin
    Antitumor activity against human RKO cell line by MTT assay
    Antitumor activity against human RKO cell line by MTT assay
    		[PMID: 16455245]
    SF-295 IC50
    0.54 μM
    Compound: Mitomycin C
    Cytotoxicity against human SF295 cells after 72 hrs by MTS assay
    Cytotoxicity against human SF295 cells after 72 hrs by MTS assay
    		[PMID: 26335269]
    SF-539 IC50
    3.8 x 10-2 μM
    Compound: Mitomycin C
    Cytotoxicity against human SF539 cells after 72 hrs by MTS assay
    Cytotoxicity against human SF539 cells after 72 hrs by MTS assay
    		[PMID: 26335269]
    Sf9 IC50
    3 μg/mL
    Compound: mitomycin C
    Cytotoxicity against Spodoptera frugiperda Sf9 cells after 24 hrs by MTT assay
    Cytotoxicity against Spodoptera frugiperda Sf9 cells after 24 hrs by MTT assay
    		[PMID: 11277765]
    SGC-7901 IC50
    2.05 μM
    Compound: Mitomycin C
    Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay
    Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay
    		[PMID: 23999045]
    SK-BR-3 IC50
    1 μM
    Compound: Mitomycin
    Cytotoxicity against human SKBR3 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human SKBR3 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay
    		[PMID: 31490685]
    SK-OV-3 GI50
    0.1 μM
    Compound: Mitomycin
    Growth inhibition of human SKOV3 cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human SKOV3 cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    SK-OV-3 IC50
    0.18 μM
    Compound: Mitomycin C
    In vitro cytotoxic activity against human ovarian carcinoma cell line SK-OV-3 after 72 hr of drug exposure determined by the SRB assay
    In vitro cytotoxic activity against human ovarian carcinoma cell line SK-OV-3 after 72 hr of drug exposure determined by the SRB assay
    		[PMID: 14980672]
    SMMC-7721 IC50
    2.44 μg/mL
    Compound: Mitomycin
    Antitumor activity against human SMMC7721 cell line by MTT assay
    Antitumor activity against human SMMC7721 cell line by MTT assay
    		[PMID: 16455245]
    SMMC-7721 IC50
    5.4 μM
    Compound: mitomycin
    Cytotoxicity against human SMMC7721 cells after 48 hrs by SRB assay
    Cytotoxicity against human SMMC7721 cells after 48 hrs by SRB assay
    		[PMID: 19432411]
    SMMC-7721 IC50
    5.6 μM
    Compound: mitomycin C
    Cytotoxicity against human SMMC7721 cells after 48 hrs by SRB assay
    Cytotoxicity against human SMMC7721 cells after 48 hrs by SRB assay
    		[PMID: 22642381]
    SW480 IC50
    0.79 μM
    Compound: Mitomycin C
    Cytotoxicity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 34847663]
    T47D IC50
    0.4 μM
    Compound: Mitomycin C
    In vitro cytotoxic activity against human breast cancer cell line T-47D after 72 hr of drug exposure determined by the SRB assay
    In vitro cytotoxic activity against human breast cancer cell line T-47D after 72 hr of drug exposure determined by the SRB assay
    		[PMID: 14980672]
    T47D IC50
    16970 nM
    Compound: MC
    Antiproliferative activity against human T47D cells after 48 hrs by resazurin reduction assay
    Antiproliferative activity against human T47D cells after 48 hrs by resazurin reduction assay
    		[PMID: 26541587]
    THP-1 IC50
    0.1 μM
    Compound: Mitomycin-C
    Cytotoxicity against human THP1 cells after 48 hrs by SRB assay
    Cytotoxicity against human THP1 cells after 48 hrs by SRB assay
    		[PMID: 25259515]
    THP-1 IC50
    0.5 μM
    Compound: Mitomycin-C
    Cytotoxicity against human THP1 cells assessed as inhibition of cell growth after 48 hrs by sulforhodamine B assay
    Cytotoxicity against human THP1 cells assessed as inhibition of cell growth after 48 hrs by sulforhodamine B assay
    		10.1039/C5MD00289C
    THP-1 IC50
    1.5 μM
    Compound: Mitomycin-C
    Cytotoxicity against Homo sapiens (human) THP1 cells after 48 hr by SRB assay
    Cytotoxicity against Homo sapiens (human) THP1 cells after 48 hr by SRB assay
    		10.1007/s00044-012-0424-0
    THP-1 IC50
    1.5 μM
    Compound: Mitomycin-C
    Cytotoxicity against human THP1 cells assessed as inhibition of cell proliferation after 48 hrs by sulforhodamine-B assay
    Cytotoxicity against human THP1 cells assessed as inhibition of cell proliferation after 48 hrs by sulforhodamine-B assay
    		10.1039/C3MD00055A
    U2OS IC50
    8.45 μM
    Compound: Mitomycin C
    Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
    		[PMID: 34847663]
    U-87MG ATCC GI50
    1.8 μM
    Compound: Mitomycin
    Growth inhibition of human U87MG cells after 72 hrs by Cell-Titer Glo assay
    Growth inhibition of human U87MG cells after 72 hrs by Cell-Titer Glo assay
    		[PMID: 28682609]
    WiDr ED50
    0.15 μg/mL
    Compound: Mitomycin C
    Cytotoxicity against human WiDr cells by MTT assay
    Cytotoxicity against human WiDr cells by MTT assay
    		[PMID: 20144547]
    WiDr ED50
    0.18 μM
    Compound: mitomycin C
    Cytotoxicity against human WiDr cells by MTT assay
    Cytotoxicity against human WiDr cells by MTT assay
    		[PMID: 19061391]
    WiDr ED50
    0.35 μg/mL
    Compound: Mitomycin
    Cytotoxicity against human WiDr cells after 72 hrs
    Cytotoxicity against human WiDr cells after 72 hrs
    		[PMID: 16643051]
    體外研究
    (In Vitro)

    HCT116 (p53-/-) 細胞對絲裂霉素 C (Ametycine) 或單獨的 TRAIL 的敏感性最低。然而，令人驚訝的是，MMC 和 TRAIL 的聯(lián)合處理顯著降低了細胞活力。盡管單獨使用絲裂霉素 C 和 TRAIL 的效果適中，但絲裂霉素 C 大大增強了 TRAIL 對抑制細胞增殖的作用。單獨使用絲裂霉素 C 和 TRAIL 處理分別誘導(dǎo) 9.5% 和 35.0% 的細胞凋亡。然而，絲裂霉素 C 和 TRAIL 聯(lián)合處理可將細胞凋亡率提高至 66.6%[1]。
    絲裂霉素 C 是一種細胞毒性化療劑，也會以 DNA 交聯(lián)的形式引起 DNA 損傷作為多種 DNA 單加合物，已知可誘導(dǎo) p53[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    攜帶異種移植 HCT116 (p53-/-) 結(jié)腸腫瘤和 HT-29 結(jié)腸腫瘤的小鼠每隔一段時間用絲裂霉素 C (阿米霉素；ip，1 mg/kg) 和 TRAIL (iv，100 μg) 處理天。用聯(lián)合處理方案的 10 個連續(xù)周期處理動物。聯(lián)合療法顯著抑制了腫瘤生長，并且不影響小鼠的體重，表明絲裂霉素 C 和 TRAIL 的處理組合具有良好的耐受性，并且在體內(nèi)具有抗腫瘤活性[1]。
    膀胱內(nèi) Mitomycin C 滴注對體重有影響。在連續(xù) 3 次每周滴注 1 mg/mL 絲裂霉素 C 后，體重幾乎沒有增加，而與 MMC 處理的大鼠相比，其他 3 組大鼠的體重增加有統(tǒng)計學(xué)意義[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    334.33

    Formula

    C15H18N4O5
    CAS 號
    性狀

    固體

    顏色

    Blue to black

    中文名稱

    絲裂霉素 C；絲裂霉素；密吐霉素；自力霉素；嘧吡霉素；突變霉素
    結(jié)構(gòu)分類
    初始來源

    Streptomyces
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    溶解性數(shù)據(jù)
    細胞實驗： 

    DMSO 中的溶解度 : 50 mg/mL (149.55 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.9911 mL 14.9553 mL 29.9106 mL
    5 mM 0.5982 mL 2.9911 mL 5.9821 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.22 mM); 澄清溶液

      此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.22 mM); 澄清溶液

      此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.98%

    參考文獻
    Cell Assay
    [1]

    Colon adenocarcinoma HCT116 and HT-29 human colon cancer cells are used. The CellTiter-Glo Luminescent Cell Viability Assay is used to measure cell viability, which use a unique, stable form of luciferase to measure ATP as an indicator of viable cells, and the luminescent signal produced is proportional to the number of viable cells present in culture. Cells are pretreated with Mitomycin C (5 μM) for 12 h or 24 h, and then exposed to different concentrations of TRAIL for 12 h. An equal volume (100 μL) of CellTiter-GloTM reagent is added and the solution is mixed gently for 2 min on an orbital shaker. Mixture is incubated at room temperature for 10 min to allow luminescent signal to stabilize, and then imaging is performed using the Xenogen IVIS system to quantify the cell viability[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1][3]

    Mice[1]
    Four- to 6-wk-old NCr nude mice are treated with Mitomycin C (1 mg/kg) by intraperitoneal injection for 24 h, followed by one intravenous dose of purified rhTRAIL (100 μg). As a negative control, a subset of the mice are injected (i.p. and i.v.) with saline (vehicle) at the same frequency of treatment. Animals are treated for 3 consecutive weeks. The tumor size is monitored every week using caliper measurements of the tumor volume.
    Rats[3]
    Young adult female Wistar rats at age 13 weeks with a median weight of 217 g (range 187 to 255) are randomized into 4 groups of 10 each, namely a normal group with no instillations, an NaCl 0.9% or placebo group that received instillations with the solvent of the chemotherapeutic agent, Mitomycin C (1 mg/mL) group.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9911 mL 14.9553 mL 29.9106 mL 74.7764 mL
    5 mM 0.5982 mL 2.9911 mL 5.9821 mL 14.9553 mL
    10 mM 0.2991 mL 1.4955 mL 2.9911 mL 7.4776 mL
    15 mM 0.1994 mL 0.9970 mL 1.9940 mL 4.9851 mL
    20 mM 0.1496 mL 0.7478 mL 1.4955 mL 3.7388 mL
    25 mM 0.1196 mL 0.5982 mL 1.1964 mL 2.9911 mL
    30 mM 0.0997 mL 0.4985 mL 0.9970 mL 2.4925 mL
    40 mM 0.0748 mL 0.3739 mL 0.7478 mL 1.8694 mL
    50 mM 0.0598 mL 0.2991 mL 0.5982 mL 1.4955 mL
    60 mM 0.0499 mL 0.2493 mL 0.4985 mL 1.2463 mL
    80 mM 0.0374 mL 0.1869 mL 0.3739 mL 0.9347 mL
    100 mM 0.0299 mL 0.1496 mL 0.2991 mL 0.7478 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Mitomycin C
    目錄號：
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