FITC-Dextran (MW 110000) is a compound belonging to the class of fluorescent dyes. It is commonly used in biomedical research as a tracer molecule to label and track cells or other biological matter. FITC-Dextran consists of fluorescein isothiocyanate (FITC) and dextran, a complex carbohydrate derived from starch. The combination of the two creates a stable fluorescent tracer that can be viewed under a microscope or quantified using specialized detection instruments.

FITC-Dextran (MW 110000) 是一種異硫氰酸熒光素 (FITC) 葡聚糖熒光探針 (Ex=495 nm; Em=525 nm)。FITC-Dextran (MW 110000) 可作為一種標記物來揭示熱休克引起的細胞損傷，并研究細胞凋亡的早期和晚期階段。FITC-Dextran (MW 110000) 還可用于細胞滲透性的研究，如血腦屏障通透性以及血腦屏障破壞程度的測定。儲存方式：避光。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

腸屏障功能測定^[5]
1. 小鼠饑餓處理 4 小時。
2. FITC-Dextran MW 110000 灌胃小鼠 (0.6 mg/g)。
3. 在 4 h 內測量熒光強度 (Ex nm/Em 520 nm)。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

110000(Average)

60842-46-8

Room temperature in continental US; may vary elsewhere.

• [1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1).

  [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.  [Content Brief]

  [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.  [Content Brief]

  [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276.  [Content Brief]

  [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282.  [Content Brief]