FITC-Dextran (MW 40000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 40000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 40000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

細胞標記^[1]:
適用于正在發(fā)生凋亡的 HeLa 細胞和人外周血單個核細胞 (PBMC) (活的 HeLa 和 PBMC 不被 FITC-Dextran 染色)。
1. 43.5°C 孵育 1 h，37°C 孵育 8 h，誘導細胞凋亡。
2. 將細胞懸浮在 100 μL 的培養(yǎng)基中，在 Q-prep 管與 10 μL 的 propidium iodide (PI)，10 μL 的 FITC-Dextran (MW 40000) 混合，PI 和 FITC-Dextran (MW 40000) 的最終濃度分別為 7.5 μM 和 1.13 μM。
3. 將細胞在室溫黑暗條件下培養(yǎng) 25 分鐘。
4. 取 3 mL 培養(yǎng)基的標記細胞，500 g 離心 10 分鐘。
5. 取離心細胞，加入 1 mL 培養(yǎng)基，用流式細胞術(shù)或熒光顯微鏡分析 (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 40000): Ex=495 nm, Em=525 nm)。


細胞旁滲透性檢測^[4]
1. 在 transwell 室的基礎(chǔ)培養(yǎng)基中加入 FITC-Dextran (0.1 mg/mL)。
2. 在 15 分鐘后從 transwell 插入器中收集培養(yǎng)基。
3. 測量熒光信號 (Ex=485 nm, Em=538 nm)。
4. 根據(jù)熒光強度計算 FITC-Dextran 濃度。
5. 計算滲透率。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

腸屏障功能測定^[5]
1. 小鼠饑餓處理 4 小時。
2. FITC-Dextran MW 40000 灌胃小鼠 (0.6 mg/g)。
3. 在 4 h 內(nèi)測量熒光強度 (Ex nm/Em 520 nm)。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

40000(Average)

60842-46-8

固體

Yellow to orange

525

488

Room temperature in continental US; may vary elsewhere.

• [1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1).

  [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.  [Content Brief]

  [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.  [Content Brief]

  [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276.  [Content Brief]

  [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282.  [Content Brief]