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  2. Immunology/Inflammation
  3. NOD-like Receptor (NLR)
  4. MCC950 sodium

MCC950 sodium  (Synonyms: CP-456773 sodium; CRID3 sodium salt)

目錄號(hào): HY-12815A 純度: 99.75%
COA 產(chǎn)品使用指南

MCC950 sodium (CP-456773 sodium; CRID3 sodium salt) 是一種有效，選擇性的 NLRP3 抑制劑，在BMDMs 和 HMDMs中的 IC50 分別為 7.5 nM 和 8.1 nM。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

MCC950 sodium Chemical Structure

MCC950 sodium Chemical Structure

CAS No. : 256373-96-3

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥660
In-stock
5 mg ￥600
In-stock
10 mg ￥910
In-stock
25 mg ￥1900
In-stock
50 mg ￥3200
In-stock
100 mg ￥3975
In-stock
200 mg ￥5813
In-stock
500 mg 現(xiàn)貨 詢價(jià)
1 g   詢價(jià)  
5 g   詢價(jià)  

* Please select Quantity before adding items.

Customer Review

Other Forms of MCC950 sodium:

MCE 顧客使用本產(chǎn)品發(fā)表的 286 篇科研文獻(xiàn)

WB
IF

    MCC950 sodium purchased from MCE. Usage Cited in: J Agric Food Chem. 2023 May 4.  [Abstract]

    MCC950 (10 μM; 2 h) markedly inhibits the expressionof NLRP3, Cleaved Caspase-1, and Cleaved IL-1β in HUVECs.

    MCC950 sodium purchased from MCE. Usage Cited in: Int J Mol Sci. 2023 Mar 27.

    MCC950 (5 μM; every other day for four weeks) significantly decreases CD68+ and GSDMD+ cells in rats.

    MCC950 sodium purchased from MCE. Usage Cited in: Journal of Traditional and Complementary Medicine. 2023 Mar 29.

    MCC950 (10 μM; 1 h) inhibits the expression of NLRP3 in THP-1 cells.

    MCC950 sodium purchased from MCE. Usage Cited in: EMBO J. 2022 Oct 17;e111173.  [Abstract]

    BMDMs isolated from Phb1F/F mice and Phb1MyeKO mice are incubated with MCC950 (50?nM) for 30?min and are subsequently stimulated by LPS (200?ng/ml) for 6?h and ATP (4?mM) for 45?min. Protein levels in whole cell lysates and culture medium are detected by WB assay.

    MCC950 sodium purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Mar 24;15(1):95.  [Abstract]

    Chronic PVN infusion of MCC950 significantly decreases apoptosis-associated speck-like protein (ASC), pro-caspase-1, and IL-1β expression in high-salt diet rats.

    MCC950 sodium purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Sep 20;9(10):946.   [Abstract]

    The efficiency of MCC950, and its effect on caspase-1 activation and IL-1β production in As2O3-treated HepG2 cells.

    MCC950 sodium purchased from MCE. Usage Cited in: Neurobiol Dis. 2018 Sep;117:15-27.  [Abstract]

    Effect of MCC950 on the expression of NLRP3 inflammasome components and substrates after traumatic brain injury (TBI).

    MCC950 sodium purchased from MCE. Usage Cited in: Biofactors. 2018 Mar;44(2):123-136.  [Abstract]

    NLRP3, Casp1 p20, IL-1b, and ICAM-1 protein levels are assessed in the cell lysates by Western blotting.

    MCC950 sodium purchased from MCE. Usage Cited in: Clin Exp Immunol. 2018 Nov;194(2):231-243.  [Abstract]

    MCC950 treatment inhibits Nucleotide-binding, oligomerization domain (NOD)-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome pathway activation.

    MCC950 sodium purchased from MCE. Usage Cited in: Molecules. 2018 Feb 27;23(3). pii: E522.  [Abstract]

    Effects of MCC950 on hippocampal NLRP3 inflammasome activation in db/db mice. Western blot analysis of NLRP3 inflammasome-associated NLRP3, ASC, IL-1β, and β-actin are performed in the hippocampus of each group.

    MCC950 sodium purchased from MCE. Usage Cited in: Dig Dis Sci. 2018 Jan;63(1):81-91.  [Abstract]

    The expression of various molecules in pyroptotic and apoptotic pathways is detected by western blot. Representative bands from densitometric analysis in experimental groups are presented. M, MCC950 group (an inhibitor of NLRP3).

    MCC950 sodium purchased from MCE. Usage Cited in: J Am Heart Assoc. 2017 Sep 4;6(9). pii: e006347.  [Abstract]

    Cells are pretreated with YVAD(10 μM) or MCC950 (10 μM) for 2 hours, and then exposed to TMAO (600 μM) for a further 24 hours. Expression of IL-1β, ICAM-1, MMP-9, and caspase-1 p20 is detected via Western blot.

    MCC950 sodium purchased from MCE. Usage Cited in: Biochim Biophys Acta. 2017 Oct 3;1864(1):1-10.  [Abstract]

    MCC950 or vehicle control (PBS) is administered at 1, 4 and 6 day after Ang II infusion in the absence or presence of EMD638683. Representative images and quantification of picrosirius red-stained collagen in the heart at 7 days after Ang II infusion.

    查看 NOD-like Receptor (NLR) 亞型特異性產(chǎn)品:

    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    MCC950 sodium (CP-456773 sodium; CRID3 sodium salt) is a potent, selective NLRP3 inhibitor with IC50s of 7.5 and 8.1 nM in BMDMs and HMDMs, respectively.

    IC50 & Target

    NLRP3

     

    體外研究
    (In Vitro)

    MCC950 在納摩爾濃度下阻斷經(jīng)典和非經(jīng)典 NLRP3 激活。MCC950 特異性抑制 NLRP3，但不抑制 AIM2、NLRC4 或 NLRP1 激活。MCC950 對(duì) NLRP3 炎性體激活的影響在小鼠骨髓來(lái)源的巨噬細(xì)胞 (BMDM) 和人單核細(xì)胞來(lái)源的巨噬細(xì)胞 (HMDM) 中進(jìn)行了測(cè)試。MCC950 在 BMDM 中的 IC50 約為 7.5 nM，而在 HMDM 中具有相似的抑制能力 (IC50=8.1 nM)。MCC950 還劑量依賴性地抑制 IL-1β 但不抑制 TNF-α 分泌。MCC950 在非經(jīng)典途徑刺激時(shí)特異性阻斷 caspase-11 定向的 NLRP3 激活和 IL-1β 分泌。NLRC4 刺激的 IL-1β 和 TNF-α 分泌 (被鼠傷寒沙門(mén)氏菌激活) 即使在 10 μM 的濃度下也不會(huì)被 MCC950 抑制。MCC950 不抑制響應(yīng)鼠傷寒沙門(mén)氏菌的 caspase-1 激活或 IL-1β 加工。pro-caspase-1 和 pro-IL-1β 在細(xì)胞裂解物中的表達(dá)基本上不受 MCC950 處理的影響[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    MCC950 減少白細(xì)胞介素-1p (IL-1β) 的產(chǎn)生并減輕實(shí)驗(yàn)性自身免疫性腦脊髓炎 (EAE) 的嚴(yán)重程度，EAE 是一種多發(fā)性硬化癥的疾病模型。用 MCC950 預(yù)處理可降低 IL-1β 和 IL-6 的血清濃度，同時(shí)不會(huì)顯著降低 TNF-α 的量。用 MCC950 處理小鼠可延遲 EAE 的發(fā)作并降低其嚴(yán)重程度。第 22 天處死的小鼠腦單核細(xì)胞的細(xì)胞內(nèi)細(xì)胞因子染色和 FACS 分析顯示，與 PBS 處理的小鼠相比，MCC950 處理的小鼠中產(chǎn)生 IL-17 和 IFN-γ 的 CD3+ T 細(xì)胞的頻率適度降低。CD3+ T 的 CD4+ 和 γδ+ 亞群中產(chǎn)生 IFN-γ 尤其是 IL-17 的細(xì)胞數(shù)量也減少[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    426.46

    Formula

    C20H23N2NaO5S
    CAS 號(hào)
    性狀

    固體

    顏色

    Off-white to yellow

    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : 100 mg/mL (234.49 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

    H2O 中的溶解度 : ≥ 30 mg/mL (70.35 mM)

    * "≥" means soluble, but saturation unknown.

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.3449 mL 11.7244 mL 23.4489 mL
    5 mM 0.4690 mL 2.3449 mL 4.6898 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

    • 方案 一

      請(qǐng)依序添加每種溶劑： 5% DMSO    95% PBS

      Solubility: ≥ 5 mg/mL (11.72 mM); 澄清溶液

    • 方案 二

      請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (5.86 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      20% SBE-β-CD in Saline 的配制（4°C，儲(chǔ)存一周）：2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑： PBS

      Solubility: 6.25 mg/mL (14.66 mM); 澄清溶液; 超聲助溶

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請(qǐng)具體參考實(shí)測(cè) 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: 99.75%

    參考文獻(xiàn)
    Cell Assay
    [1]

    BMDM are seeded at 5×105/mL or 1×106/mL, HMDM at 5×105/mL and PBMC at 2×106/mL or 5×106/mL in 96 well plates. The following day the overnight medium is replaced and cells are stimulated with 10 ng/mL LPS from Escherichia coli serotype EH100 (ra) TLRgrad for 3 h. Medium is removed and replaced with serum free medium (SFM) containing DMSO (1:1,000), MCC950 (0.001-10 μM), Parthenolide (10 μM) or Bayer cysteinyl leukotriene receptor antagonist 1-(5-carboxy-2{3-[4-(3-cyclohexylpropoxy)phenyl]propoxy}benzoyl)piperidine-4-carboxylic acid (40 μM) for 30 min. Cells are then stimulated with inflammasome activators: 5 mM adenosine 5’-triphosphate disodium salt hydrate (ATP) (1 h), 1 μg/mL Poly(deoxyadenylic-thymidylic) acid sodium salt (Poly dA:dT) transfected with Lipofectamine 200 (3-4 h), 200 μg/mL MSU (overnight) and 10 μM nigericin (1 h) or S. typhimurium UK-1 strain. Cells are also stimulated with 25 μg/mL Polyadenylic-polyuridylic acid (4 h). For non-canonical inflammasome activation cells are primed with 100 ng/mL Pam3CSK4 for 4 h, medium is removed and replaced with SFM containing DMSO or MCC950 and 2 μg/mL LPS is transfected using 0.25% FuGENE for 16 h. Supernatants are removed and analysed using ELISA kits. LDH release is measured using the CytoTox96 non-radioactive cytotoxicity assay[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice[1]
    C57BL/6 mice are immunized subcutaneously with 150 μg of MOG peptide 35-55 emulsified in CFA containing 4 mg/mL (0.4.mg/mouse) of heat-killed MTB. Mice are injected i.p. with 500 ng pertussis toxin (PT: kaketsuken) on days 0 and 2. MCC950 is administered i.p. to mice (10 mg/kg) at induction of the disease, day 0, 1 and 2 and every 2 days thereafter. Control mice are administered vehicle (PBS) at the same time points. Mice are observed for clinical signs of disease daily (unblinded). Disease severity is scored as follows: no clinical signs, 0; limp tail, 1; ataxic gait, 2; hind limb weakness, 3; hind limb paralysis, 4; and tetra paralysis, 5.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.3449 mL 11.7244 mL 23.4489 mL 58.6221 mL
    5 mM 0.4690 mL 2.3449 mL 4.6898 mL 11.7244 mL
    10 mM 0.2345 mL 1.1724 mL 2.3449 mL 5.8622 mL
    15 mM 0.1563 mL 0.7816 mL 1.5633 mL 3.9081 mL
    20 mM 0.1172 mL 0.5862 mL 1.1724 mL 2.9311 mL
    25 mM 0.0938 mL 0.4690 mL 0.9380 mL 2.3449 mL
    30 mM 0.0782 mL 0.3908 mL 0.7816 mL 1.9541 mL
    40 mM 0.0586 mL 0.2931 mL 0.5862 mL 1.4656 mL
    50 mM 0.0469 mL 0.2345 mL 0.4690 mL 1.1724 mL
    60 mM 0.0391 mL 0.1954 mL 0.3908 mL 0.9770 mL
    DMSO 80 mM 0.0293 mL 0.1466 mL 0.2931 mL 0.7328 mL
    100 mM 0.0234 mL 0.1172 mL 0.2345 mL 0.5862 mL

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    MCC950 sodium
    目錄號(hào)：
    HY-12815A
    需求量：