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  2. Metabolic Enzyme/Protease
  3. Oxidative Phosphorylation Mitochondrial Metabolism
  4. BAM 15

BAM 15 是線粒體質(zhì)子解偶聯(lián)劑。BAM 15 是氧化磷酸化 (OXPHOS) 解偶聯(lián)劑。

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BAM 15 Chemical Structure

BAM 15 Chemical Structure

CAS No. : 210302-17-3

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥830
In-stock
1 mg ￥340
In-stock
5 mg ￥750
In-stock
10 mg ￥1234
In-stock
25 mg ￥2475
In-stock
50 mg ￥3975
In-stock
100 mg ￥5738
In-stock
200 mg   詢價  
500 mg   詢價  

* Please select Quantity before adding items.

Customer Review

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

BAM 15 is a mitochondrial protonophore uncoupler. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupler[1].

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
L6 EC50
0.31 μM
Compound: 8; BAM15
Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay
Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay
[PMID: 32392051]
L6 EC50
0.31 μM
Compound: BAM15
Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer
Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer
[PMID: 35907607]
L6 EC50
0.49 μM
Compound: 5; BAM15
Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay
Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay
[PMID: 32017849]
MCF7 EC50
0.31 μM
Compound: BAM15
Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 33569941]
體外研究
(In Vitro)

BAM 15 is able to increase O2 consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1 μM increase cellular O2 consumption rate (OCR) to a similar degree as FCCP, but higher concentrations from 1 μM to 50 μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling, demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

Compare to vehicle-treated mice, animals that receive BAM 15 are protected from kidney injury as indicated by lower plasma creatinine levels at 24 and 48 h post-ischemia, reduced tubular necrosis, less depletion of brush border villi, less obstruction of proximal tubules, and less immune cell infiltration[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

340.29

Formula

C16H10F2N6O
CAS 號
性狀

固體

顏色

Light yellow to yellow

運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
溶解性數(shù)據(jù)
細(xì)胞實驗： 

DMSO 中的溶解度 : 50 mg/mL (146.93 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.9387 mL 14.6933 mL 29.3867 mL
5 mM 0.5877 mL 2.9387 mL 5.8773 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.35 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.92%

參考文獻(xiàn)
Kinase Assay
[1]

Electron flow assays are performed. Briefly, 5 μg of mitochondrial protein in MAS is loaded into a 24-well tissue culture plate and centrifuged at 2000×g for 15 min at 4°C. Prior to the assay, mitochondria are incubated at 37°C for 10 mins in MAS containing 10 mM pyruvate, 2 mM malate, and 5 μM BAM 15 or FCCP. Rotenone (2 μM), succinate (10 mM), antimycin A (4 μM), and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD, 100 μM) plus ascorbate (10 mM) are added sequentially as indicated in the figure. N=3 wells/plate of a representative of 3 plates[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[1]

L6 cells are incubated with the fluorescent indicator of mitochondrial membrane potential tetramethylrhodamine (TMRM, 125 nM) or DMSO (1%) control for 30 min. The cells are then centrifuged for 5 min at 700×g and resuspended in unbuffered DMEM at a concentration of 1×105 cells/mL. The cells are then treated with BAM 15 or DMSO (0.1%) for 10 min prior to flow cytometric analysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Male mice (8-week old, C57BL/6) are used. Mice are i.p. injected with BAM 15 at 1 or 5 mg/kg, 1 h before kidney IR. Vehicle mice are also injected with the same solution BAM 15 is prepared with (3% DMSO in 50% PEG400)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9387 mL 14.6933 mL 29.3867 mL 73.4667 mL
5 mM 0.5877 mL 2.9387 mL 5.8773 mL 14.6933 mL
10 mM 0.2939 mL 1.4693 mL 2.9387 mL 7.3467 mL
15 mM 0.1959 mL 0.9796 mL 1.9591 mL 4.8978 mL
20 mM 0.1469 mL 0.7347 mL 1.4693 mL 3.6733 mL
25 mM 0.1175 mL 0.5877 mL 1.1755 mL 2.9387 mL
30 mM 0.0980 mL 0.4898 mL 0.9796 mL 2.4489 mL
40 mM 0.0735 mL 0.3673 mL 0.7347 mL 1.8367 mL
50 mM 0.0588 mL 0.2939 mL 0.5877 mL 1.4693 mL
60 mM 0.0490 mL 0.2449 mL 0.4898 mL 1.2244 mL
80 mM 0.0367 mL 0.1837 mL 0.3673 mL 0.9183 mL
100 mM 0.0294 mL 0.1469 mL 0.2939 mL 0.7347 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
BAM 15
目錄號：
HY-110284
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