Bryostatin 3, a macrocyclic lactone, is a protein kinase C activator, with a K_i of 2.75 nM. Bryostatin 3 can block 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibition of cell proliferation, yet did not block TPA-enhanced cell-substratum adhesion^[1][2].

Ki: 2.75 nM (protein kinase C)^[1]

Bryostatin 3 (1 μM; 24 h) does not inhibit [3Hlthymidine incorporation and largely blocks the growth-inhibitory action of TPA^[2].
Bryostatin 3 (1 μM; 24 h) blocks the action of TPA to inhibit cell proliferation but does not block TPA-enhanced cell-substratum adhesion^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

888.99

C₄₆H₆₄O₁₇

143370-84-7

Room temperature in continental US; may vary elsewhere.

• [1]. Wender PA, et, al. Modeling of the bryostatins to the phorbol ester pharmacophore on protein kinase C. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7197-201.  [Content Brief]

  [2]. Mackanos EA, et, al. Bryostatins selectively regulate protein kinase C-mediated effects on GH4 cell proliferation. J Biol Chem. 1991 Jun 15;266(17):11205-12.  [Content Brief]