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  2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. MAFP

MAFP  (Synonyms: Methyl Arachidonyl Fluorophosphonate)

目錄號: HY-103334 純度: 98.6%
COA 產(chǎn)品使用指南 技術支持

MAFP (Methyl Arachidonyl Fluorophosphonate) 是選擇性，針對活性位點，不可逆的 cPLA2 和 iPLA2 抑制劑。 MAFP也是一種有效的不可逆的 anandamide amidase 抑制劑。

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MAFP Chemical Structure

MAFP Chemical Structure

CAS No. : 188404-10-6

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規(guī)格 價格 是否有貨 數(shù)量
1 mg (26.99 mM * 100 μL in Methyl acetate) ￥1350
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查看 Phospholipase 亞型特異性產(chǎn)品:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

MAFP (Methyl Arachidonyl Fluorophosphonate) is an selective, active-site directed and irreversible inhibitor of cPLA2 and iPLA2. MAFP is also a potent irreversible inhibitor of anandamide amidase.

IC50 & Target

cPLA2, iPLA2[1], Anandamide amidase[2]
體外研究
(In Vitro)

在 P388D1 細胞中 40°C 預孵育 5 分鐘后，MAFP 以濃度依賴性方式抑制 iPLA2，IC50 為 0.5 μM。cPLA是一種以228位絲氨酸殘基的羥基為催化親核試劑的磷脂水解酶[1]。
MAFP 也是 anandamide 酰胺酶的抑制劑，并且是 CB1 大麻素受體的配體。MAFP 表現(xiàn)出對 anandamide 酰胺酶的選擇性，其效力分別比對胰凝乳蛋白酶和胰蛋白酶的效力高約 3000 和 30000 倍。MAFP 取代[3H]CP-55940 與 CB1 大麻素受體的結合，IC50 為 20 nM vs 40 nM for anandamide[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

370.48

Formula

C21H36FO2P
CAS 號
性狀

液體（密度：0.960±0.06 g/cm3）

顏色

Colorless to light yellow

運輸條件

Shipping with dry ice.
儲存方式

-80°C, protect from light
溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : ≥ 100 mg/mL (269.92 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.6992 mL 13.4960 mL 26.9920 mL
5 mM 0.5398 mL 2.6992 mL 5.3984 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months (protect from light)。-80°C 儲存時，請在6個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: 98.6%

參考文獻
Kinase Assay
[1]

MAFP is dissolved and diluted in DMSO. To investigate the reversibility of iPLA 2 inhibition by MAFP, the P388D1 iPLA 2 is first concentrated approximately 10-fold using a Centricon-10 concentrator from Amicon. The concentrated iPLA 2 (20 μL) is then preincubated with either 80 μM MAFP in DMSO or DMSO alone (2 μL) for 5 min at 40°C. A 2 μL aliquot is removed and subsequently diluted 1500-fold into 3 mL of assay mixture containing 100 μM DPPC (200000 cpm per 50 μL assay mixture), 400 μM Triton X-100, 100 mM Hepes (pH 7.5), 5 mM EDTA, 1 mM DTT and 0.8 mM ATP. At the indicated time points, a 50 μL aliquot is removed and the remaining enzyme activity is quantified[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[2]

Inhibition of anandamide amidase in cell culture is measured using approximately 1x106 Nl8TG2 intact neuroblastoma cells. Experimental cells are preincubated for 20 min in 1.5 mL medium, consisting of Fl2/DMEM with penicillin, streptomycin, gentamicin, 10% bovine calf serum, plus MAFP (1, 5, 10, 20 nM). Control cells contained no inhibitor. Arachidonoyl is then added and the incubation continued for I hr. The amount of [3H]anandamide in the cells is quantified by liquid scintillation counting of the silica scraped from the appropriate areas of the TLC plate identified by exposure to X-ray film[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months (protect from light)。-80°C 儲存時，請在6個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6992 mL 13.4960 mL 26.9920 mL 67.4800 mL
5 mM 0.5398 mL 2.6992 mL 5.3984 mL 13.4960 mL
10 mM 0.2699 mL 1.3496 mL 2.6992 mL 6.7480 mL
15 mM 0.1799 mL 0.8997 mL 1.7995 mL 4.4987 mL
20 mM 0.1350 mL 0.6748 mL 1.3496 mL 3.3740 mL
25 mM 0.1080 mL 0.5398 mL 1.0797 mL 2.6992 mL
30 mM 0.0900 mL 0.4499 mL 0.8997 mL 2.2493 mL
40 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6870 mL
50 mM 0.0540 mL 0.2699 mL 0.5398 mL 1.3496 mL
60 mM 0.0450 mL 0.2249 mL 0.4499 mL 1.1247 mL
80 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8435 mL
100 mM 0.0270 mL 0.1350 mL 0.2699 mL 0.6748 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
MAFP
目錄號：
HY-103334
需求量：