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  3. Dihydrofluorescein diacetate

Dihydrofluorescein diacetate  (Synonyms: 二氫熒光素二乙酸酯)

目錄號: HY-101893 純度: 98.63%
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Dihydrofluorescein diacetate是用于無細(xì)胞系統(tǒng)和細(xì)胞模型中測量氧化應(yīng)激的熒光探針。

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Dihydrofluorescein diacetate Chemical Structure

Dihydrofluorescein diacetate Chemical Structure

CAS No. : 35340-49-9

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MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.

體外研究
(In Vitro)

Dihydrofluorescein diacetate 是許多基于細(xì)胞研究的優(yōu)良熒光探針。它是用于檢測細(xì)胞內(nèi)氧化劑的熒光探針,因為它對特定的氧化物質(zhì)更具反應(yīng)性。Dihydrofluorescein diacetate 在復(fù)氧內(nèi)皮細(xì)胞中表現(xiàn)出線性結(jié)構(gòu)的熒光,與線粒體一致[1]。
Dihydrofluorescein diacetate 能夠檢測線粒體中 ROS 的存在。Dihydrofluorescein diacetate 的熒光是尖銳的和劃定的細(xì)絲,其所有細(xì)節(jié)都與 TMRM 染色的線粒體一致。它進入線粒體并與基質(zhì)中釋放的 ROS 發(fā)生反應(yīng)[2]。
Dihydrofluorescein diacetate 可能是一種有用的定量方法,用于測量納米顆粒處理的細(xì)胞的氧化電位[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

418.40

Formula

C24H18O7

CAS 號
性狀

固體

顏色

White to yellow

Emission (Em)

616

Excitation (Ex)

518

中文名稱

二氫熒光素二乙酸酯

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實驗: 

DMSO 中的溶解度 : ≥ 106.5 mg/mL (254.54 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響,請使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.3901 mL 11.9503 mL 23.9006 mL
5 mM 0.4780 mL 2.3901 mL 4.7801 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時,請在6個月內(nèi)使用,-20°C儲存時,請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息:

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

由于實驗過程有損耗,建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料
染色示例
參考文獻
Cell Assay

Dihydrofluorescein diacetate is used at a final concentration of 5 μM for the cell-free studies. The excitation wavelength is 488 nm and the emission wavelength is 521 nm, the same as the excitation and emission wavelengths of the confocal microscope. All fluorescence measurements are performed on room temperature solutions. Human umbilical vein endothelium at first passage are cultured to confluence on 48 well plates. Cells are ished with PBS, then PBS with glucose, pH 7.4, is added to the cells. Dihydrofluorescein diacetate is added at a final concentration of 20 mM. Wells without cells are also loaded with fluorescent probes to serve as controls. Fluorescence measurements are made on a fluorescent platereader every 10 min at Exλ 488 nm and Emλ 510 (bandpass 5 nm) and 538 nm (bandpass 10 nm). The Emλ 510 nm and Emλ 538 nm values are added together[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時,請在6個月內(nèi)使用,-20°C儲存時,請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3901 mL 11.9503 mL 23.9006 mL 59.7514 mL
5 mM 0.4780 mL 2.3901 mL 4.7801 mL 11.9503 mL
10 mM 0.2390 mL 1.1950 mL 2.3901 mL 5.9751 mL
15 mM 0.1593 mL 0.7967 mL 1.5934 mL 3.9834 mL
20 mM 0.1195 mL 0.5975 mL 1.1950 mL 2.9876 mL
25 mM 0.0956 mL 0.4780 mL 0.9560 mL 2.3901 mL
30 mM 0.0797 mL 0.3983 mL 0.7967 mL 1.9917 mL
40 mM 0.0598 mL 0.2988 mL 0.5975 mL 1.4938 mL
50 mM 0.0478 mL 0.2390 mL 0.4780 mL 1.1950 mL
60 mM 0.0398 mL 0.1992 mL 0.3983 mL 0.9959 mL
80 mM 0.0299 mL 0.1494 mL 0.2988 mL 0.7469 mL
100 mM 0.0239 mL 0.1195 mL 0.2390 mL 0.5975 mL
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產(chǎn)品名稱:
Dihydrofluorescein diacetate
目錄號:
HY-101893
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