DMOG

別名: Dimethyloxalylglycine

目錄號：S7483 Purity: 99.96%

DMOG (Dimethyloxalylglycine)是α-酮戊二酸輔助因子的拮抗劑和HIF prolylhydroxylase的抑制劑，它使HIF-1α蛋白在核內(nèi)穩(wěn)定并積累，是HIF-1α的激動劑。DMOG 可增加自噬。在溶液中不穩(wěn)定，請現(xiàn)配現(xiàn)用！

DMOG Chemical Structure

CAS: 89464-63-1

規(guī)格	價格	庫存
20mg	794.43	現(xiàn)貨
100mg	2678.13	現(xiàn)貨
1g	7944.3	現(xiàn)貨
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產(chǎn)品質(zhì)控

批次：純度： 99.96%

99.96

DMOG相關(guān)產(chǎn)品

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細(xì)胞實驗數(shù)據(jù)示例

細(xì)胞系	實驗類型	給藥濃度	活性描述	文獻(xiàn)信息
human 293T cells?	Function assay	2.5 mM	Inhibition of JMJD2-mediated H3K36 demethylation in human 293T cells at 2.5 mM	19359167
human SW982 cell	Growth inhibition assay		Inhibition of human SW982 cell growth in a cell viability assay, IC50=33.7007 μM	SANGER
human OPM-2 cell	Growth inhibition assay		Inhibition of human OPM-2 cell growth in a cell viability assay, IC50=28.8227 μM	SANGER
human KU812 cell	Growth inhibition assay		Inhibition of human KU812 cell growth in a cell viability assay, IC50=28.4579 μM	SANGER
human CAL-12T cell	Growth inhibition assay		Inhibition of human CAL-12T cell growth in a cell viability assay, IC50=26.5502 μM	SANGER
human COR-L105 cell	Growth inhibition assay		Inhibition of human COR-L105 cell growth in a cell viability assay, IC50=26.4436 μM	SANGER
human LC-2-ad cell	Growth inhibition assay		Inhibition of human LC-2-ad cell growth in a cell viability assay, IC50=24.5573 μM	SANGER
human YT cell	Growth inhibition assay		Inhibition of human YT cell growth in a cell viability assay, IC50=23.3984 μM	SANGER
human KYSE-410 cell	Growth inhibition assay		Inhibition of human KYSE-410 cell growth in a cell viability assay, IC50=20.8197 μM	SANGER
human CAL-39 cell	Growth inhibition assay		Inhibition of human CAL-39 cell growth in a cell viability assay, IC50=19.3277 μM	SANGER
human DMS-273 cell	Growth inhibition assay		Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=15.965 μM	SANGER
human BL-70 cell	Growth inhibition assay		Inhibition of human BL-70 cell growth in a cell viability assay, IC50=15.9423 μM	SANGER
human A3-KAW cell	Growth inhibition assay		Inhibition of human A3-KAW cell growth in a cell viability assay, IC50=15.8174 μM	SANGER
human U-698-M cell	Growth inhibition assay		Inhibition of human U-698-M cell growth in a cell viability assay, IC50=13.3306 μM	SANGER
human KS-1 cell	Growth inhibition assay		Inhibition of human KS-1 cell growth in a cell viability assay, IC50=11.2557 μM	SANGER
human LOUCY cell	Growth inhibition assay		Inhibition of human LOUCY cell growth in a cell viability assay, IC50=10.3493 μM	SANGER
human LB996-RCC cell	Growth inhibition assay		Inhibition of human LB996-RCC cell growth in a cell viability assay, IC50=5.6877 μM	SANGER
human SR cell	Growth inhibition assay		Inhibition of human SR cell growth in a cell viability assay, IC50=4.57946 μM	SANGER
human HH cell	Growth inhibition assay		Inhibition of human HH cell growth in a cell viability assay, IC50=34.0235 μM	SANGER
human RS4-11 cell	Growth inhibition assay		Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=34.1806 μM	SANGER
human BEN cell	Growth inhibition assay		Inhibition of human BEN cell growth in a cell viability assay, IC50=36.8187 μM	SANGER
human CCRF-CEM cell	Growth inhibition assay		Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=37.7729 μM	SANGER
human EW-13 cell	Growth inhibition assay		Inhibition of human EW-13 cell growth in a cell viability assay, IC50=39.0481 μM	SANGER
human NCI-H2228 cell	Growth inhibition assay		Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=42.818 μM	SANGER
human KE-37 cell	Growth inhibition assay		Inhibition of human KE-37 cell growth in a cell viability assay, IC50=43.2915 μM	SANGER
human A4-Fuk cell	Growth inhibition assay		Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=45.0364 μM	SANGER
human MZ1-PC cell	Growth inhibition assay		Inhibition of human MZ1-PC cell growth in a cell viability assay, IC50=45.9888 μM	SANGER
human MLMA cell	Growth inhibition assay		Inhibition of human MLMA cell growth in a cell viability assay, IC50=46.3292 μM	SANGER
human EB-3 cell	Growth inhibition assay		Inhibition of human EB-3 cell growth in a cell viability assay, IC50=49.5792 μM	SANGER
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生物活性

產(chǎn)品描述

靶點(diǎn)

HIF prolyl hydroxylase ^[1]

體外研究(In Vitro)
體外研究活性	DMOG在微粒體系統(tǒng)中僅有很低的活性，但在完整細(xì)胞中可強(qiáng)烈抑制羥基脯胺酸合成。^[1]在HPASMC中，DMOG通過抑制脯氨酰羥化酶降低FGF-2誘導(dǎo)的組織和cyclin A表達(dá)。^[3]
細(xì)胞實驗	細(xì)胞系	VSMC細(xì)胞
	濃度	~100 μM
	孵育時間	72-96 小時
	方法	為分析DNA合成作為細(xì)胞增殖的指數(shù)，VSMC細(xì)胞以5000個每平方厘米的密度接種在48孔板培養(yǎng)基中，孵育過夜，除去血清（1%FCS）處理24小時。復(fù)制的孔板儲存−70°C 作為基線（第0天）細(xì)胞計數(shù)，含有或者不含有生長因子的新鮮培養(yǎng)基加入孔板中，在20%或5%的氧氣中孵育72-96小時。第0，3，4天，通過在含有熒光染料的緩沖液中裂解細(xì)胞測定細(xì)胞數(shù)，其自身具有很弱的熒光性，但與DNA或RNA結(jié)合時會增強(qiáng)熒光。細(xì)胞數(shù)目的定量是通過比較樣品的熒光與已知細(xì)胞數(shù)目的標(biāo)準(zhǔn)曲線比較來測定的。
實驗圖片	檢測方法	檢測指標(biāo)	實驗圖片	PMID
	Western blot	HIF-1α p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM WNT11 / HIF-2α		29555474
	Immunofluorescence	HIF1α		28955984

體內(nèi)研究(In Vivo)
體內(nèi)研究活性	在小鼠局部缺血的骨骼肌中，DMOG抑制內(nèi)源性HIF失活，誘導(dǎo)血管新生。^[2]在高血脂癥大鼠體內(nèi)，通過DMOG上調(diào)的缺氧誘導(dǎo)因子1a能夠增強(qiáng)缺血后處理的心臟保護(hù)作用。^[4]
動物實驗	Animal Models	小鼠(C57Bl6)
	Dosages	8毫克在0.5毫升生理鹽水中
	Administration	腹腔注射

參考文獻(xiàn)
[1] Baader E, et al. Biochem J. 1994, 300 ( Pt 2), 525-530. [2] Milkiewicz M, et al. J Physiol. 2004, 60(Pt 1), 21-26. [3] Schultz K, et al. Am J Physiol Lung Cell Mol Physiol. 2009, 296(6), L921-927. [4] Li X, et al. Acta Biochim Biophys Sin (Shanghai). 2014, 46(2), 112-118. + 展開

參考文獻(xiàn)

[1] Baader E, et al. Biochem J. 1994, 300 ( Pt 2), 525-530.
[2] Milkiewicz M, et al. J Physiol. 2004, 60(Pt 1), 21-26.
[3] Schultz K, et al. Am J Physiol Lung Cell Mol Physiol. 2009, 296(6), L921-927.

[4] Li X, et al. Acta Biochim Biophys Sin (Shanghai). 2014, 46(2), 112-118.

+ 展開