Berberine chloride

別名: NSC 646666, Natural Yellow 18 chloride 中文名稱：鹽酸小檗堿，黃連素

目錄號：S2271 Purity: 99.75%

Berberine chloride是異喹啉類生物堿的季銨鹽。Berberine chloride 可激活 caspase 3 和 caspase 8，分裂 poly ADP-ribose polymerase (PARP) 和釋放 cytochrome c。Berberine chloride 可降低 c-IAP1，Bcl-2 和 Bcl-XL 的表達。Berberine chloride 可通過 JNK 和 p38 MAPK 的持續(xù)磷酸化以及產生 ROS 來誘導凋亡。Berberine chloride 是一個 topoisomerase I 和 II 的雙效抑制劑。Berberine chloride 也是一個潛在的自噬調節(jié)劑。

Berberine chloride Chemical Structure

CAS: 633-65-8

規(guī)格	價格	庫存
10mM (1mL in DMSO)	744.27	現(xiàn)貨
10mg	571.33	現(xiàn)貨
50mg	794.43	現(xiàn)貨
200mg	1040.13	現(xiàn)貨
1g	5217.03	現(xiàn)貨
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產品質控

批次：純度： 99.75%

99.75

Berberine chloride相關產品

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細胞實驗數(shù)據(jù)示例

細胞系	實驗類型	給藥濃度	孵育時間	活性描述	文獻信息
MRC5 cells	Proliferation assay	10 μM	54 hrs	Inhibition of HCMV proliferation in MRC5 cells after 54 hrs post-infection at 10 uM by plaque assay	17239594
MRC5 cells	Proliferation assay	10 μM	24 h	Inhibition of HCMV proliferation in MRC5 cells after 24 hrs post-infection at 10 uM by plaque assay	17239594
HepG2 cells	Function assay	10 ug/mL	12 h	Induction of LDLR protein expression in human HepG2 cells at 10 ug/mL after 12 hrs by flow cytometry	19090767
A10 cells	Function assay	30 μM	24 hrs	Downregulation of Scd2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis	21401114
A10 cells	Function assay	30 μM	24 hrs	Down regulation of Prim2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis	21401114
A10 cells	Function assay	30 μM	24 hrs	Downregulation of Impk mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis	21401114
HepG2 cells	Function assay	10 μM	4 h	Increase in AMPKalpha phosphorylation in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control	23058107
HepG2 cells	Function assay	10 μM	4 h	Increase in total AMPKalpha level in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control	23058107
HepG2 cells	Function assay	20 μM	24 hrs	Induction of apoptosis in human HepG2 cells assessed as morphological changes at 20 uM after 24 hrs using Hoechst 33258 staining by fluorescence microscopic analysis	23182088
HepG2-A16-CD81 cells	Function assay	10 μM		NOVARTIS: Antimalarial liver stage activity measured as a greater than 50% reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells at 10uM compound concentration, determined by immuno-fluorescence.	22096101
HepG2-A16-CD81 cells	Function assay	10 μM		NOVARTIS: Antimalarial liver stage activity measured as reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells by immuno-fluorescence, and median schizont size at 10uM compound concentration, IC50=0.548 μM	22096101
Bel7402 cells	Function assay		12 h	Induction of LDLR protein in human Bel7402 cells after 12 hrs by RT-PCR assay relative to control	19090767
KB cells	Cytotoxicity assay		72 h	Cytotoxicity against human KB cells after 72 hrs, IC50=7.32 μM	11141105
HL60 cells	Apoptosis assay		48 hrs	Induction of apoptosis in human HL60 cells after 48 hrs using annexin V-propidium iodide staining by FACS analysis	20227144
CEM cells	Cytotoxicity assay		48 hrs	Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay, CC50=2.09 μM	21295891
human CEM cells	Function assay		7 days	Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA, EC50=0.13 μM	21295891
SKN cells	Growth inhibition assay		72 h	Growth inhibition against human SKN cells after 72 hrs by MTT assay, GI50=15.88 μM	21401114
RKN cells	Growth inhibition assay		48 hrs	Growth inhibition against human RKN cells after 48 hrs by MTT assay, GI50=49.6 μM	21401114
G402 cells	Growth inhibition assay		48 hrs	Growth inhibition against human G402 cells after 48 hrs by MTT assay, GI50=11.87 μM	21401114
HT-29 cells	Cytotoxicity assay		48 hrs	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay, IC50=8.45 μM	23182088
HepG2 cells	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50=11.22 μM	23182088
HepG2 cells	Cytotoxicity assay		48 hrs	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay, IC50=8.32 μM	23182088
MRC5 cells	Function assay			Antiviral activity against HCMV in MRC5 cells by plaque reduction assay, IC50=0.68 μM	17239594
A549 cells	Cytotoxicity assay			Cytotoxicity against human A549 cells by SRB assay, IC50=6.27 μM	20594848
SKOV3 cells	Cytotoxicity assay			Cytotoxicity against human SKOV3 cells by SRB assay, IC50=16.44 μM	20594848
SK-MEL-2 cells	Cytotoxicity assay			Cytotoxicity against human SK-MEL-2 cells by SRB assay, IC50=13.76 μM	20594848
HCT15 cells	Cytotoxicity assay			Cytotoxicity against human HCT15 cells by SRB assay, IC50=16.59 μM	20594848
點擊查看更多細胞系數(shù)據(jù)

生物活性

產品描述

靶點

Caspase-3 ^[1]

Caspase-8 ^[1]

PARP ^[1]

cytochrome c ^[1]

cIAP1 ^[1]

點擊更多

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試驗信息 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06273241	Not yet recruiting	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	March 4 2024	Not Applicable
NCT05845931	Recruiting	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	May 5 2023	Not Applicable
NCT05480670	Completed	Polycystic Ovary Syndrome	Ayub Teaching Hospital	November 1 2022	Not Applicable
NCT05463003	Completed	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	July 19 2022	Not Applicable

參考文獻
[1] Wen-Hsiu Hsu, et al. Arch Toxicol. 2007 Oct;81(10):719-28. [2] Marta Cernáková, et al. BMC Complement Altern Med. 2002 Feb 19;2:2. [3] Wang K, et al. Front Pharmacol. 2021;12:632201.

參考文獻

[1] Wen-Hsiu Hsu, et al. Arch Toxicol. 2007 Oct;81(10):719-28.
[2] Marta Cernáková, et al. BMC Complement Altern Med. 2002 Feb 19;2:2.
[3] Wang K, et al. Front Pharmacol. 2021;12:632201.

化學信息&溶解度

分子量	371.81	分子式	C₂₀H₁₈NO₄.Cl
CAS號	633-65-8	SDF	Download Berberine chloride SDF
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復凍融！	1年-80°C溶于溶劑
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復凍融！	1個月-20°C溶于溶劑

體外溶解度
批次：

DMSO : 25 mg/mL ( (67.23 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : Insoluble

Ethanol : Insoluble

DMSO : 38 mg/mL ( (102.2 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩爾濃度計算器

體內溶解度
批次：

現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑

動物體內配方計算器

實驗計算

摩爾濃度計算器

質量	濃度	體積	分子量
=	×	×

稀釋計算器分子量計算器

動物體內配方計算器（澄清溶液）

第一步：請輸入基本實驗信息（考慮到實驗過程中的損耗，建議多配一只動物的藥量）

給藥劑量 mg/kg 動物平均體重 g 每只動物給藥體積 μL 動物數(shù)量只

第二步：請輸入動物體內配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

計算結果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；

體內配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH₂O，混勻澄清。

體內配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

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