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CAS RN: | 產(chǎn)品編碼: D5909
DAB staining Kit
純度/分析方法:
- DAB染色試劑盒
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補充產(chǎn)品信息:
Product Information
Components : DAB solution, Hydrogen Peroxide solution, 1.5M Tris-HCl buffer
Size : 100 tests (About 500 mL substrate solution)
Description
This kit consists three reagents; 3, 3’-diaminobenzidine (DAB) solution, Hydrogen Peroxide solution and 1.5M Tris-HCl buffer and it is supplied to detect horseradish peroxidase (HRP) activity in Western blotting and so on. When DAB reacts with HRP, a brown precipitate appears. This kit does not include nickel component.
Direction for Use
1) Prepare a blotting membrane covered with HRP-conjugated antibody.
2) Prepare the DAB substrate solution according to the following volume;
? - Add 5 drops of DAB solution (about 140 μL)
? - Add 2 drops of Hydrogen Peroxide solution (about 80 μL)
? - Add 2 drops of 1.5M Tris-HCl buffer (about 80 μL)
? - Each reagent is added into 5 mL of deionized water
3) Soak the membrane in the DAB substrate solution and incubate until the membrane is colored.
4) Add deionized water and stop the coloring reaction.
Storage
The product should be stored at 4 °C and used within 6 months.
產(chǎn)品編碼 | D5909 |
外觀與形狀(20°C) | 液體 |
儲存溫度 | 冷藏 (0-10°C) |
應(yīng)避免的情況 | 加熱 |
監(jiān)管條件代碼(*) |
Figure. Dot blot image of substrate solutions prepared by DAB staining kit reacting with HRP (from left to right: 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078 (mU/spot)).
References
- Assessment of a method for immunochemical detection of antigen on nitrocellulose membranes.
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
References
- Studies on Microperoxisomes V. Are Microperoxisomes Ubiquitous in Mammalian Cells?
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