Thiolutin
Antibiotic; inhibits bacterial RNA polymerase. Inhibits adhesion of HUVEC cells to vitronectin (IC50 = 0.83 mM) and subsequently reduces paxillin levels. Suppresses tumor cell-induced angiogenesis.
| Physical Appearance | A crystalline solid |
| Storage | Desiccate at -20°C |
| M.Wt | 228.28 |
| Cas No. | 87-11-6 |
| Formula | C8H8N2O2S2 |
| Solubility | Limited solubility, soluble in DMSO |
| Chemical Name | N-(4-methyl-5-oxodithiolo[4,3-b]pyrrol-6-yl)acetamide |
| SDF | Download SDF |
| Canonical SMILES | CC(=O)NC1=C2C(=CSS2)N(C1=O)C |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Human umbilical vein endothelial cells (HUVECs), as well as breast carcinoma (MCF-7), prostate carcinoma (PC3), ovarian carcinoma (CaOV3), and small cell lung carcinoma (OH-1) cells |
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Reaction Conditions |
0.05 ~ 500 μM thiolutin for 72 h incubation |
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Applications |
Thiolutin potently inhibited HUVEC proliferation at 0.1 μM. The potent antiproliferative activity of thiolutin was selective for endothelial cells in that several other tumor cell lines showed weaker inhibition of proliferation by thiolutin (i.e., at 10 μM), and a small cell lung carcinoma cell line was completely resistant. Thus, thiolutin appeared to be a selective inhibitor of endothelial cell proliferation. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Dai S, Jia Y, Wu SL, et al. Comprehensive characterization of heat shock protein 27 phosphorylation in human endothelial cells stimulated by the microbial dithiole thiolutin. Journal of Proteome Research, 2008, 7(10): 4384-4395. |
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Quality Control & MSDS
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Chemical structure
