Cell lines

Splenic T cells

Reaction Conditions

0.1, 1 and 10 μM paroxetine

Applications

Paroxetine (1?μM and 10?μM) pretreatment distinctly restrained T cell migration induced by CX3CL1 through inhibiting G protein-coupled receptor kinase 2 (GRK2). Paroxetine also inhibited GRK2 induced activation of ERK. Thus, paroxetine could potentially attenuate the symptoms of collagen-induced arthritis due to its inhibitory effect on T cell activation and infiltration to synovial tissue via suppression of ERK pathway.

Animal models

Male CFY rats, 125 ~ 250 g

Dosage form

0.3, 1, 3 and 6 mg/kg

Oral administration

Applications

Paroxetine HCl dose-dependently prevented the 5-HT depleting effect of p-chloroamphetamine (PCA) in rat brain, demonstrating 5-HT uptake blockade in vivo. At the highest dose tested (6 mg/kg), paroxetine HCl completely prevented the PCA effect. Paroxetine HCl (0.3 and 6.0 mg/kg), administered in the absence of PCA, produced significant increases in brain 5-HT concentrations. Therefore, paroxetine HCl could provide a useful pharmacological tool for investigating 5-HT systems.

Note

The technical data provided above is for reference only.

References:

1. Wang Q, Wang L, Wu L, et al. Paroxetine alleviates T lymphocyte activation and infiltration to joints of collagen-induced arthritis. Scientific Reports, 2017, 7: 45364.

2. Thomas DR, Nelson DR, Johnson AM. Biochemical effects of the antidepressant paroxetine, a specific 5-hydroxytryptamine uptake inhibitor. Psychopharmacology (Berl), 1987, 93(2): 193-200.