Tetracycline Hydrochloride is an inhibitor of bacterial ribosomes [1].?

Tetracycline Hydrochloride is believed to inhibit translation by binding to the 16S rRNA and inhibiting the binding of aminoacyl-tRNA to the mRNA-ribosome complex. However, the exact target site(s) and mechanism(s) of action remain subjects of much debate [1].?

Tetracycline Hydrochloride at doses of 0, 10, 25, 50, 75, 150, 225 and 300 μM exhibited increasing antimicrobial effect against resistant strains of Staphylococcus aureus (S. aureus). The statistically evaluated IC50 values of Tetracycline Hydrochloride against S. aureus, cadmium-resistant S. aureus, and lead-resistant S. aureus after first 6 h were 4.8, 3.8, 2.2 μM, respectively [2].?

In humans who had minimum Propionibacterium acnes (P. acnes) levels of 10000 colony-forming units per cm² at baseline, Tetracycline Hydrochloride treatment (1000 mg/d, b.i.d., p.o.) over a 6-week period significantly reduced P. acnes on both cheeks and forehead after 3 and 6 weeks, but produced little to no residual effect. At 9 weeks, P. acnes levels in the Tetracycline Hydrochloride group had returned to baseline [3].?

References:

[1]. Chukwudi C U. rRNA Binding Sites and the Molecular Mechanism of Action of the Tetracyclines. Antimicrobial Agents and Chemotherapy, 2016, 60(8): 4433-4441.

[2]. Chudobova D, Dostalova S, Blazkova I, et al. Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus. International Journal of Environmental Research and Public Health, 2014, 11(3): 3233-3255.

[3]. Leyden J J, Kaidbey K, Gans E H. The antimicrobial effects in vivo of minocycline, doxycycline and tetracycline in humans. Journal of Dermatological Treatment, 1996, 7: 223-225.