Cell lines

Chinese hamster ovary cell CHO

Preparation method

The solubility of this compound in DMSO is ≥9.6 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

25, 50, 100 and 200 μg/ml

Applications

In the absence of the S-9 mixture, as the concentration of nitrofurazone increased, the major trends of chromosomal gaps, breaks and swaps in CHO cells increased. The incidence of chromatid damage was also increased in cells treated with nitrofurazone, but there was no significant dose correlation. In the presence of a mixture of S-9, an increase in chromosomal damage was accompanied by an increase in the dose correlation of chromatid breaks and interchanges.

Animal models

Male rats

Dosage form

40,120 and 400 mg/kg (administration by gavage)

Application

After nitrofurazone treatment, there was no evidence of any increase in the percentage of cell aberrations, the average number of chromosomal aberrations, or the number of animal abnormalities at any sampling time (single or five doses per day).

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Kong D, Yun H, Cui D, Qi M, Shao C, Cui D, Ren N, Liang B, Wang A. Response of antimicrobial nitrofurazone-degrading biocathode communities to different cathode potentials. Bioresour Technol. 2017 Oct; 241:951-958. doi:10.1016/j.biortech.2017.06.056. Epub 2017 Jun 13. PubMed PMID: 28637162.