PMSF
PMSF (Phenylmethanesulfonyl fluoride) is an irreversible inhibitor of serine proteinases, which is associated with the development of the delayed organophosphorus neuropathy. It has a role in a lot of cellular repair and regeneration processes in many kinds of tissues. PMSF is a long acting Neuropathy Target Esterase (NTE) inhibitor. NTE is a protection was related to inhibition of the putative target of organophosphate-induced delayed polyneuropathy (OPIDP). PMSF can increase NTE inhibition to more than 90%. PMSF also acts as an active site directed reagent for γ-glutamyl transpeptidase.
Reference
Thomas Baker, Herbert E. Lowndes, Martin K. Johnson, Irene C. Sandborg. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology. 1980; 46(3-4): 305 – 311.
Marcello Lotti, Stefano Caroldi, Eugenio Capodicasa, Angelo Moretto. Promotion of organophosphate-induced delayed polyneuropathy by phenylmethanesulfonyl fluoride. Toxicology and Applied Pharmacology. 1991; 108(2): 234 – 241.
Masayasu Inoue, Seikoh Horiuchi, Yoshimasa Morino. Inactivation of γ-glutamyl transpeptidase by phenylmethanesulfonyl fluoride, a specific inactivator of serine enzymes. Biochemical and Biophysical Research Communications. 1978; 82(4): 1183 – 1188.
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| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 174.2 |
| Cas No. | 329-98-6 |
| Formula | C7H7FO2S |
| Solubility | insoluble in H2O; ≥17.4 mg/mL in DMSO; ≥28.3 mg/mL in EtOH |
| Chemical Name | phenylmethanesulfonyl fluoride |
| SDF | Download SDF |
| Canonical SMILES | C1=CC=C(C=C1)CS(=O)(=O)F |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Longitudinal smooth muscle of guinea pig ileum |
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Reaction Conditions |
2 mM PMSF for 30 min incubation |
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Applications |
PMSF inhibited carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15% ~ 19%. The inhibition effect of PMSF on phosphoinositide turnover was due to one or more steps following phosphoinositide breakdown. |
| Animal experiment:[2] | |
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Animal models |
Cats |
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Dosage form |
30 mg/kg Administered intraperitoneally (i.p.) |
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Applications |
Pretreatment of cats with PMSF (30 mg/kg i.p.) 24 h before the diisopropylfluorophosphate (DFP) injection protected the cats from the delayed neuropathy. No clinical neurotoxic signs were observed at 21 days after DFP exposure. The stimulus-bound repetitive capacity of soleus α-motor nerve terminals was not lost at this time and its incidence was much greater than that which occurred in cats not pretreated with PMSF. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Sekar MC, Roufogalis BD. Differential effects of phenylmethanesulfonyl fluoride (PMSF) on carbachol and potassium stimulated phosphoinositide turnover and contraction in longitudinal smooth muscle of guinea pig ileum. Cell Calcium, 1984, 5(3): 191-203. 2. Baker T, Lowndes HE, Johnson MK, et al. The effects of phenylmethanesulfonyl fluoride on delayed organophosphorus neuropathy. Archives of Toxicology, 1980, 46(3-4): 305-311. |
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