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AICAR

Catalog No.
A8184
AMPK activator
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$61.00
In stock
50mg
$55.00
In stock
200mg
$143.00
In stock
1g
$407.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

AICAR is a cell-permeable, allosteric activator of AMP-activated protein kinase (AMPK).

AMPK is a heterodimeric protein serine/threonine kinase that regulates the energy status of cells to protect cell from metabolic stress. AMPK phosphorylates various metabolic enzymes to activate catabolic pathways (e.g. ketogenesis) and block anabolic pathways (e.g. protein synthesis).

In rat primary astrocytes, microglia, and peritoneal macrophages, AICAR does-dependently inhibits the LPS-induced production of TNFα, IL-1β, and IL-6. AICAR treatment also blocks LPS-induced nitrite production and iNOS gene expression in those cells in a dose-dependent manner by activation of AMPK. Moreover, AICAR inhibits the LPS-induced C/EBP nuclear relocation via downregulating the expression of C/EBP-δ. [1]

In LPS-injected rats, AICAR treatment abolishes LPS-mediated increased levels of IL-1β and IFN-γ in serum. AICAR treatment also strongly inhibits the LPS-induced expression of iNOS in peritoneal macrophages isolated from these rats. Furthermore, the intraperitoneal injection of LPS significantly induces the expression of TNFα, IL-1β, and IFN-γ message in the rat spleen.

Reference:
1.? Giri S, Nath N, Smith B et al. 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside inhibits proinflammatory response in glial cells: a possible role of AMP-activated protein kinase. J Neurosci. 2004 Jan 14;24(2):479-87.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt258.23
Cas No.2627-69-2
FormulaC9H14N4O5
SynonymsGP 1-110
Solubilityinsoluble in EtOH; ≥12.9 mg/mL in DMSO; ≥52.9 mg/mL in H2O
Chemical Name5-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]imidazole-4-carboxamide
SDFDownload SDF
Canonical SMILESCC(C=C(C)C=CC(=O)NO)C(=O)C1=CC=C(C=C1)N(C)C
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:

Cell lines

Rat primary astrocytes, microglia and peritoneal macrophages

Preparation method

The solubility of this compound in DMSO is limited. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20 °C for several months.

Reaction Conditions

0.01 ~ 1 mM; 2 hrs

Applications

In rat primary astrocytes, microglia and peritoneal macrophages, AICAR does-dependently inhibited the LPS-induced production of TNFα, IL-1β and IL-6.
Animal experiment [1]:

Animal models

LPS-injected rats

Dosage form

100 mg/kg; i.p.

Applications

In LPS-injected rats, AICAR abolished LPS-mediated increased levels of IL-1β and IFN-γ in serum.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Giri S, Nath N, Smith B et al. 5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside inhibits proinflammatory response in glial cells: a possible role of AMP-activated protein kinase. J Neurosci. 2004 Jan 14;24(2):479-87.

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