Poly(I:C)
Poly (I:C), a synthetic double-stranded RNA (dsRNA) analog, is an immunostimulant that acts as the most potent interferon (IFN) inducer. Toll-like receptor- 3 (TLR3) agonist[1]. Its average molecular weight is between 1.5-8kb.
Mixtures that included the TLR7/8 agonists R848 or CL075, combined with the Poly (I:C), yield 3-d mature dendritic cells [2].
Commonly used to stimulate immune system activation in vivo. Also promotes maturation of hPSC-derived cardiomyocytes[3].?
References:
[1]. Li Y G, Siripanyaphinyo U, Tumkosit U, et al. Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells. Virology Journal, 2012, 9(1): 114.
[2]. Spranger S, Javorovic M, Burdek M, et al. Generation of Th1-Polarizing Dendritic Cells Using the TLR7/8 Agonist CL075. The Journal of Immunology, 2010, 185(1): 738-747.
[3]. Biermann M1, Cai W2, Lang D, et al. Epigenetic Priming of Human Pluripotent Stem Cell-Derived Cardiac Progenitor Cells Accelerates Cardiomyocyte Maturation. Stem Cells, 2019, 37(7): 910-923.
- 1. Qing Zhao, Yanjing Zhu, et al. "Targeting resident astrocytes attenuates neuropathic pain after spinal cord injury." Elife. 2024 Nov 15:13:RP95672. PMID: 39545839
- 2. Weiwen Fan, Wenkai Li, et al. "Bifunctional HDAC and DNMT inhibitor induces viral mimicry activates the innate immune response in triple-negative breast cancer." Eur J Pharm Sci. 2024 Jun 1:197:106767. PMID: 38636781
- 3. Yixuan He, Baocai Gang, et al. "ACE2 improves endothelial cell function and reduces acute lung injury by downregulating FAK expression." Int Immunopharmacol. 2024 Feb 15:128:111535. PMID: 38246001
- 4. Chengyue Wu, Mengdong Wang, et al. "TRIM26 facilitates PRV infection through NDP52-mediated MAVS autophagic degradation." Research Square. 02 Nov, 2023.
- 5. Qiufan Xu, Jianpeng Liang, et al. "Newcastle disease virus nucleocapsid protein mediates the degradation of 14-3-3ε to antagonize the interferon response and promote viral replication." Vet Microbiol. 2023 Sep;284:109851. PMID: 37598526
- 6. Fan-Lian Zeng, Xiao-Yan Wang, et al. "Interleukin‐37 promotes DMBA/TPA skin cancer through SIGIRR‐mediated inhibition of glycolysis in CD103+DC cells." MedComm (2020). 2023 Mar 5;4(2):e229. PMID: 36891351
- 7. Liu Y, Ge X, et al. "Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus)." In Vitro Cell Dev Biol Anim 2023 Feb 21 PMID: 36809593
- 8. Zhaoyi Pan, Yujie Feng, et al. "MERS-CoV nsp1 impairs the cellular metabolic processes by selectively downregulating mRNAs in a novel granules." Virulence. 2022 Dec;13(1):355-369. PMID: 35129074
- 9. Tao Chen, Yu-Xin Lin, et al. "A Low-Producing Haplotype of Interleukin-6 Disrupting CTCF Binding Is Protective against Severe COVID-19." mBio. 2021 Oct 26;12(5):e0137221. PMID: 34634929
- 10. Yueyan Liao, Xiangyu Ke, et al. "The Second-Generation XPO1 Inhibitor Eltanexor Inhibits Human Cytomegalovirus (HCMV) Replication and Promotes Type I Interferon Response." Front Microbiol. 2021 May 3;12:675112. PMID: 34012430
- 11. Lin Lv , Mingzhu Cao, et al. "PRV-encoded UL13 protein kinase acts as an antagonist of innate immunity by targeting IRF3-signaling pathways." Vet Microbiol. 2020 Nov;250:108860. PMID: 33045632
- 12. Song Y, Li P, et al. "CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription." Cell Mol Immunol. 2019 Nov 15. PMID: 31729464
- 13. Qi C, Wang X, et al. "Arginine supplementation improves growth, antioxidant capacity, immunity and disease resistance of juvenile Chinese mitten crab, Eriocheir sinensis." Fish Shellfish Immunol. 2019 Jul 30. pii: S1050-4648(19)30792-2. PMID: 31374316
- 14. Chistyakov DV, Azbukina NV, et al. "Toll-like receptors control p38 and JNK MAPK signaling pathways in rat astrocytes differently, when cultured in normal or high glucose concentrations." Neurochem Int. 2019 Jul 29:104513. PMID: 31369777
| Physical Appearance | A solid (HMW) |
| Storage | Store at -20°C |
| Cas No. | 24939-03-5 |
| Formula | (C10H13N4O8P)x.(C9H14N3O8P)x |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥21.5 mg/mL in H2O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
Dendritic cell |
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Preparation method |
Soluble in sterile water. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
12.5 mg/ml, 3 days |
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Applications |
Treatment of immature DC for 72 h with poly(I:C) resulted in the typical DC morphology and induced significant maturation. Poly(I:C)-treated DC remained stable in phenotype after cytokine withdrawal. Poly(I:C)-treated DC down-regulated pinocytic activity. Poly(I:C)-treated DC produced high levels of IL-12 and low levels of IL-10. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Verdijk R M, Mutis T, Esendam B, et al. Polyriboinosinic polyribocytidylic acid (poly (I: C)) induces stable maturation of functionally active human dendritic cells[J]. The Journal of Immunology, 1999, 163(1): 57-61. |
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Quality Control & MSDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
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