α-Amanitin

Catalog No.

A4548

RNA polymerase II inhibitor

Grouped product items
Size	Price	Stock	Qty
1mg	$311.00	In stock

For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Background

α-Amanitin,?the?most?deleterious?toxin?of?A.?phalloides?to?humans,?inhibits?RNA?polymerase?II?(RNAPII),?causing?hepatic?and?renal?failure.

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	918.97
Cas No.	23109-05-9
Formula	C₃₉H₅₄N₁₀O₁₄S
Solubility	≥1 mg/mL in H2O; Soluble in Ethanol
SDF	Download SDF
Canonical SMILES	CCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Cell experiment:[1]
Cell lines	Mouse blastocysts
Reaction Conditions	1.1 μg/ml α-amanitin
Applications	α-Amanitin inhibited the incorporation of ³H-UMP into embryos. α-Amanitin at the concentration of 1.1 μg/ml was found to inhibit RNA polymerase activity by 32%.
Animal experiment:[1]
Animal models	Preimplantation mouse embryos at the two-cell stage of development collected from CF1 female mice
Dosage form	0 ~ 1 μg/ml α-amanitin 48 h
Applications	Less than 1% of the embryos which had been treated with 1 μg/ml of α-amanitin developed to the morula or blastocyst stages. α-Amanitin can be used to determine which forms of RNA polymerase are active and the relative amounts of each form which are present in preimplantation mouse embryos.
Note	The technical data provided above is for reference only.
	References: 1. Warner CM, Versteegh LR. In vivo and in vitro effect of alpha-amanitin on preimplantation mouse embryo RNA polymerase. Nature, 1974, 248(5450): 678-680.

Purity ≥90%