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Calcein AM (Calcein acetoxymethyl ester)

Catalog No.
B7755
A cell-permeable dye, converted to its fluorescent form after entering live cells
Grouped product items
SizePriceStock Qty
1mg
$206.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Calcein AM (calcein acetoxymethyl ester) is a non-fluorescent, uncharged molecule, which is freely permeable to the plasma membrane of viable cells. Upon entering live cells, calcein AM is cleaved by nonspecific esterases in the cytoplasm to form membrane-impermeant calcein, a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively. Calcein AM has wide-ranging applications in the fields of cell viability, cell-cell communication and cytotoxicity. In addition, calcein is a substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3), which makes calcein AM a useful tool for studying these proteins and their regulation.?

References:

1.?Miles FL, Lynch JE, Sikes RA. Cell-based assays using calcein acetoxymethyl ester show variation in fluorescence with treatment conditions. Journal of Biological Methods, 2015, 2(3): e29.

2.?Liu X, Zhu XY, Chen M, et al. Resveratrol protects PC12 cells against OGD/R-induced apoptosis via the mitochondrial-mediated signaling pathway. Acta Biochimica et Biophysica Sinica, 2016, 48(4): 342-353.

3.?Polli JW, Wring SA, Humphreys JE, et al. Rational use of in vitro P-glycoprotein assays in drug discovery. Journal of Pharmacology and Experimental Therapeutics, 2001, 299(2): 620-628.

4.?Tiberghien F, Loor F. Ranking of P-glycoprotein substrates and inhibitors by a calcein-AM fluorometry screening assay. Anticancer Drugs, 1996, 7(5): 568-578.

5.?Szakács G, Jakab K, Antal F, et al. Diagnostics of multidrug resistance in cancer. Pathology & Oncology Research, 1998, 4(4): 251-257.

Product Citation

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt994.86
Cas No.148504-34-1
FormulaC46H46N2O23
SolubilitySoluble in DMSO
Chemical Nametetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate
SDFDownload SDF
Canonical SMILESO=C1O[C@@]2(C3=CC=CC=C31)C(C=C4)=C(C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4OC(C)=O)OC5=C2C=CC(OC(C)=O)=C5CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Quality Control & MSDS

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Chemical structure

Calcein AM (Calcein acetoxymethyl ester)