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Levofloxacin
Levofloxacin, a synthetic fluoroquinolone, is an antibacterial agent that inhibits the supercoiling activity of bacterial DNA gyrase, halting DNA replication.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 361.37 |
| Cas No. | 100986-85-4 |
| Formula | C18H20FN3O4 |
| Solubility | insoluble in H2O; ≥36.19 mg/mL in DMSO; ≥2.82 mg/mL in EtOH with ultrasonic |
| Chemical Name | (S)-9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-3,7-dihydro-2H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid |
| SDF | Download SDF |
| Canonical SMILES | FC1=C(C2=C(C3=C1[H])N(C([H])=C(C(O[H])=O)C3=O)[C@](C([H])([H])[H])([H])C([H])([H])O2)N4C([H])([H])C([H])([H])N(C([H])([H])[H])C([H])([H])C4([H])[H] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
Osteoblastic cells |
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Preparation method |
The solubility of this compound in DMSO is ≥11.4 mg/ml. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
80 μg/ml |
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Applications |
Levofloxacin has the smallest inhibitory effect on osteoblast growth, with a concentration of approximately 80 mg / mL at 50% inhibition at 48 and 72 hours. Levofloxacin strongly inhibits calcium deposition, as can be determined by alizarin red staining and biochemical analysis on day 14. |
| Animal experiment [1]: | |
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Animal models |
Juvenile New Zealand White rabbits |
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Dosage form |
100 mg/kg (p.o.), for 7 days |
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Application |
Levofloxacin initially inhibited the synthesis of glycosaminoglycans, followed by the inhibition of DNA synthesis and mitochondrial function in cultured rabbit chondrocytes at actual arthritic concentrations, but these changes were reversible and were not sufficient to kill the cells. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Holtom PD, Pavkovic SA, Bravos PD, Patzakis MJ, Shepherd LE, Frenkel B.Inhibitory effects of the quinolone antibiotics trovafloxacin, ciprofloxacin, and levofloxacin on osteoblastic cells in vitro. J Orthop Res. 2000 Sep;18(5):721-7. PubMed PMID: 11117292. [2]. Kato M, Takada S, Ogawara S, Takayama S. Effect of levofloxacin on glycosaminoglycan and DNA synthesis of cultured rabbit chondrocytes at concentrations inducing cartilage lesions in vivo. Antimicrob Agents Chemother.1995 Sep;39(9):1979-83. PubMed PMID: 8540702; PubMed Central PMCID: PMC162867. |
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Quality Control & MSDS
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Chemical structure
