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CAS No. : | 93-56-1 | MDL No. : | MFCD00003546 |
Formula : | C8H10O2 | Boiling Point : | - |
Linear Structure Formula : | HOCH(C6H5)CH2OH | InChI Key : | PWMWNFMRSKOCEY-UHFFFAOYSA-N |
M.W : | 138.16 | Pubchem ID : | 7149 |
Synonyms : |
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Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P280-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H302 | Packing Group: | N/A |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With ketoreductase; stereospecific carbonyl reductase 1; zinc(II) sulfate; In aq. phosphate buffer; at 30℃; for 8h;pH 7.0;Enzymatic reaction; | The biocatalytic deracemization of racemic (R,S)-PED was performed by the following methods: a reaction mixture (2.0 mL) containing 1gL-1 (R,S)-PED, 1 mM ZnSO4, and the purified enzymes of SCR1 and KRD. The reaction was performed at 30C and 200 rpm for 8 h. The analytical methods were carried out as described above. The reaction parameters, including activity ratio of SCR1 (20-100 U) to KRD (20-100 U), molar ratio of NADP+ (0.05-0.2 mM) to NADPH (0.05-0.2 mM), and substrate concentration (1-5 gL-1), were evaluated for the deracemization of (R,S)-PED to (R)-enantiomer. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
In hexane; isopropyl alcohol; at 35℃;Resolution of racemate;Product distribution / selectivity; | 1HNMR (400 MHz, CD3COCD3) δ 3.53 (dd, J=2 Hz, 4 Hz, 1H, CHHOH), 3.64 (dd, J=2 Hz, 10 Hz, 1H, CHHOH), 4.04 (br, H, OH), 4.43 (br, 1H, OH), 4.73 (dd, J=4 Hz, 10 Hz, 1H, CHOH), 7.21-7.40 (m, 5H, aromatic H); HPLC (CHIRALCEL OB; solvent, hexane/2-propanol=98/2; flow rate, 1.0 ml/min; temperature, 35 C.; UV wavelength, 220 nm); tR of both optical isomers of 1-phenyl-1,2-ethanediol, 26.0 minutes and 36.2 minutes. In the reaction, the optical isomer detected at 26.0 minutes was a main component, but R and S isomers were not identified. |
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