[ CAS No. 65-46-3 ] {[proInfo.proName]}

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2D 3D

Structure of 65-46-3 * Storage: {[proInfo.prStorage]}

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Quality Control of [ 65-46-3 ]

COA NMR CNMR HPLC LC-MS

Related Doc. of [ 65-46-3 ]

SDS Specification

Alternatived Products of [ 65-46-3 ]

Product Details of [ 65-46-3 ]

CAS No. :	65-46-3	MDL No. :	MFCD00006545
Formula :	C₉H₁₃N₃O₅	Boiling Point :	-
Linear Structure Formula :	HOCH₂C₄H₄O(OH)₂C₄H₂N₂(O)NH₂	InChI Key :	UHDGCWIWMRVCDJ-XVFCMESISA-N
M.W :	243.22	Pubchem ID :	6175
Synonyms :	Cytosine β-D-riboside;Cytosine-1-β-D-ribofuranoside;NSC 20258;β-D-Cytidine	Chemical Name :	4-Amino-1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidin-2(1H)-one

Safety of [ 65-46-3 ]

Signal Word:	Warning	Class:	N/A
Precautionary Statements:	P261-P280-P301+P312-P302+P352-P305+P351+P338	UN#:	N/A
Hazard Statements:	H302-H315-H319-H335	Packing Group:	N/A
GHS Pictogram:

Application In Synthesis of [ 65-46-3 ]

* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.

Upstream synthesis route of [ 65-46-3 ]
Downstream synthetic route of [ 65-46-3 ]

[ 65-46-3 ] Synthesis Path-Upstream 1~7

1
[ 17287-03-5 ]
[ 65-46-3 ]
[ 20594-00-7 ]
[ 2140-72-9 ]
[ 2140-64-9 ]

Reference： [1] Journal of Organic Chemistry, 1980, vol. 45, # 19, p. 3865 - 3868
[2] Journal of Organic Chemistry, 1980, vol. 45, # 19, p. 3865 - 3868
[3] Journal of Organic Chemistry, 1980, vol. 45, # 19, p. 3865 - 3868
[4] Journal of the Chemical Society, Perkin Transactions 1, 1980, p. 2787 - 2792
[5] Journal of the Chemical Society, Perkin Transactions 1, 1980, p. 2787 - 2792

2
[ 65-46-3 ]
[ 74-88-4 ]
[ 2140-72-9 ]

Reference： [1] Patent: US5646265, 1997, A,

3
[ 684-93-5 ]
[ 65-46-3 ]
[ 2140-72-9 ]
[ 2140-64-9 ]

Reference： [1] Angewandte Chemie - International Edition, 2018, vol. 57, # 20, p. 5943 - 5946[2] Angew. Chem., 2018, vol. 130, # 20, p. 6050 - 6054,5

4
[ 17287-03-5 ]
[ 65-46-3 ]
[ 20594-00-7 ]
[ 2140-72-9 ]

Reference： [1] Bulletin of the Chemical Society of Japan, 1986, vol. 59, p. 2947 - 2949
[2] Bulletin of the Chemical Society of Japan, 1986, vol. 59, p. 2947 - 2949

5
[ 1899-02-1 ]
[ 65-46-3 ]
[ 2140-69-4 ]
[ 20594-00-7 ]
[ 2140-72-9 ]

Reference： [1] Synthetic Communications, 1980, vol. 10, # 1, p. 25 - 36
[2] Synthetic Communications, 1980, vol. 10, # 1, p. 25 - 36

6
[ 65-46-3 ]
[ 74-88-4 ]
[ 20594-00-7 ]
[ 2140-72-9 ]

Reference： [1] Journal of Organic Chemistry, 1991, vol. 56, # 20, p. 5846 - 5859
[2] Journal of Organic Chemistry, 1991, vol. 56, # 20, p. 5846 - 5859

7
[ 186581-53-3 ]
[ 65-46-3 ]
[ 20594-00-7 ]
[ 2140-72-9 ]

Reference： [1] Helvetica Chimica Acta, 1996, vol. 79, # 8, p. 2114 - 2136
[2] Carbohydrate Research, 1981, vol. 91, p. 31 - 38
[3] Carbohydrate Research, 1981, vol. 91, p. 31 - 38

[ 65-46-3 ] Synthesis Path-Downstream 1~10

1
[ 65-46-3 ]
[ 1147-23-5 ]

Yield	Reaction Conditions	Operation in experiment
85.1%	With iodine; iodic acid; acetic acid; In tetrachloromethane; water; at 20 - 40℃;	Synthesis of 5-iodocytidine 191: A mixture of cytidine 190 (15.0 g, 61.7 mmol) in 225 mL of acetic acid and 225 mL of carbon tetrachioride was warmed to 40 CC, and iodine (9.6 g, 75.7 mmol) was added.To the stirred reaction mixture was added slowly a solution of iodic acid (9.6 g, 54.6 mmol) in 25 mL of water within 10 mm. The reaction mixture was stirred at 40 CC for 6 h and stirred at room temperature overnight. Upon completion of the reaction as monitored by TLC, the reaction mixture was concentrated under reduced pressure. The residue was purified by flash chromatography on a silica gel column using dichloromethane-methanol(15:1 to 10:1 to5:1) asgradienteluents resulting in 19.4g (85.1%) desiredproduct 5-iodocytidine (191).
85.1%	With iodine; iodic acid; In tetrachloromethane; water; acetic acid; at 40℃; for 6h;	Synthesis of 5-iodocytidine 1 91 : A mixture of cytidine 190 (15.0 g, 61 .7 mmol) in 225 mL of acetic d and 225 mL of carbon tetrachloride was warmed to 40 C, and iodine (9.6 g, 75.7 mmol) was added. the stirred reaction mixture was added slowly a solution of iodic acid (9.6 g, 54.6 mmol) in 25 mL of ter within 10 min. The reaction mixture was stirred at 40 C for 6 h and stirred at room temperature ernight. Upon completion of the reaction as monitored by TLC, the reaction mixture was concentrated der reduced pressure. The residue was purified by flash chromatography on a silica gel column using hloromethane-methanol (15:1 to 1 0:1 to 5:1 ) as gradient eluents resulting in 1 9.4 g (85.1 %) desired duct 5-iodocytidine (191 ).
85.1%	With iodine; iodic acid; acetic acid; In tetrachloromethane; water; at 20 - 40℃;	Synthesis of 5-iodocytidine 191: A mixture of cytidine 190 (15.0 g, 61.7 mmol) in 225 mL of acetic acid and 225 mL of carbon tetrachloride was warmed to 40 C, and iodine (9.6 g, 75.7 mmol) was added. To the stirred reaction mixture was added slowly a solution of iodic acid (9.6 g, 54.6 mmol) in 25 mL of water within 10 min. The reaction mixture was stirred at 40 C for 6 h and stirred at room temperature overnight. Upon completion of the reaction as monitored by TLC, the reaction mixture was concentrated under reduced pressure. The residue was purified by flash chromatography on a silica gel column using dichloromethane-methanol (15:1 to 10:1 to 5:1) as gradient eluents resulting in 19.4 g (85.1 %) desired product 5-iodocytidine (191).

72.4%	With N-iodo-succinimide; In methanol; at 40 - 70℃;	The cytidine (0.66 g, 2.7 mol) was added to the three-necked flask,N-iodosuccinimide (0.60 g, 2.4 mol) and 50 ml of methanol, and the temperature was raised to 40-70 C. The reaction was stirred for 6-12 hours.Cooled to room temperature, filtered, added with silica gel and removed by rotary evaporation. The column chromatography gave 0.48 g of a pale yellow solid in 72.4% yield.
51%	With iodine; iodic acid; acetic acid; In tetrachloromethane; water; at 40℃; for 2h;	General procedure: The suspension of nucleosides 2a,b (19 mmol) in water (5.7 mL) was treated with HIO3(9.7 mmol, 1.7 g), AcOH (15.2 mL) and a solution of iodine (11.22 mmol, 2.85 g) inCCl4 (3.8 mL). The resulting mixture was stirred at 40C for 2 h until the starting materialwas consumed or some by-product was formed (monitored by HPLC). After that,water (20 mL) was added. The reaction mixture was cooled to 4C and filtered. The precipitatewas washed with water (2 ￡ 10 mL). The combined solutions were diluted withwater (250 ml) and extracted with benzene (3 ￡ 150 mL). The aqueous layer was evaporatedunder reduced pressure. The product was purified by RPC in a linear gradient ofEtOH in water (0-30%) to give the product 3a,b.

Reference： [1]Patent: WO2015/196130,2015,A2 .Location in patent: Page/Page column 634; 635
[2]Patent: WO2015/196128,2015,A2 .Location in patent: Page/Page column 642
[3]Patent: EP2918275,2015,A1 .Location in patent: Paragraph 2219; 2220
[4]Journal of the American Chemical Society,2015,vol. 137,p. 34 - 37
[5]Synthesis,2003,p. 1039 - 1042
[6]Patent: CN106674316,2017,A .Location in patent: Paragraph 0015-0016; 0019-0020
[7]Synthesis,2009,p. 3957 - 3962
[8]Tetrahedron Letters,1999,vol. 40,p. 8961 - 8964
[9]Organic Preparations and Procedures International,2018,vol. 50,p. 332 - 342
[10]Chemical and pharmaceutical bulletin,1982,vol. 30,p. 2688 - 2697

2
[ 628-36-4 ]
[ 65-46-3 ]
[ 104105-76-2 ]

Reference： [1]Journal of Organic Chemistry,1995,vol. 60,p. 7066 - 7069

3
[ 628-36-4 ]
[ 65-46-3 ]
[ 172323-29-4 ]

Reference： [1]Journal of Organic Chemistry,1995,vol. 60,p. 7066 - 7069

4
[ 58066-85-6 ]
[ 65-46-3 ]
n-Hexadecylphosphorylcytidine [ No CAS ]

Reference： [1]Journal of Physical Chemistry B,1998,vol. 102,p. 8686 - 8691

5
[ 65-46-3 ]
[ 7075-11-8 ]

Reference： [1]Chemical and Pharmaceutical Bulletin,1980,vol. 28,p. 2915 - 2923

6
[ 5578-73-4 ]
[ 65-46-3 ]
2C₉H₁₃N₃O₅*C₂₀H₁₄NO₄⁽¹⁺⁾*Cl^(1-) [ No CAS ]

Reference： [1]Asian Journal of Chemistry,2012,vol. 24,p. 809 - 812

7
[ 1147-23-5 ]
[ 65-46-3 ]

Reference： [1]Chemistry - A European Journal,2019,vol. 25,p. 1773 - 1780

8
[ 65-46-3 ]
[ 10212-25-6 ]

Yield	Reaction Conditions	Operation in experiment
47.7%	With [1,3]-dioxolan-2-one; In N,N-dimethyl-formamide; at 82.0℃; for 2.0h;	Add 100g cytidine and 500ml N to the three-neck bottle.N-dimethylformamide,After stirring and dispersing evenly,Add 410g of ethylene carbonate,Turn on heating,Warming up to 82 C,After basic dissolution,start the timer,Reaction 2h; TLC showed that the starting material reaction was complete,Stop heating,Naturally cool and stir,When cooling to 40 C,Drop 200ml of methanol,After the addition is completed,Stir at room temperature for 1.2 h,Then the solvent was distilled off under reduced pressure; 2500 ml of methanol was added to the basic evaporation.Beating at room temperature for 3 hours,Filter under reduced pressure,Rinse twice with ethyl acetate,The amount of ethyl acetate used is 200ml each time.The filter cake was dried under vacuum at 65 C for 4 h; the gray solid powder was obtained.Weighing 51.4g,The yield is 47.7%.Based on cytidine,The purity is 79%.

Reference： [1]Patent: CN109422788,2019,A .Location in patent: Paragraph 0101-0103

9
[ 321574-29-2 ]
[ 65-46-3 ]
N⁴-[O-(4-trifluoromethylbenzyloxy)]cytidine [ No CAS ]

Reference： [1]Journal of Medicinal Chemistry,2019,vol. 62,p. 3677 - 3695

10
[ 321574-29-2 ]
[ 65-46-3 ]
N⁴-[O-(4-trifluoromethylbenzyloxy)]-cytidine-5'-O-[(phosphonomethyl)phosphonic acid] ditriethylamine salt [ No CAS ]

Reference： [1]Journal of Medicinal Chemistry,2019,vol. 62,p. 3677 - 3695

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[ 65-46-3 ]

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Reason: Stable Isotope

Ghs Precautionary Statements

Precautionary Statements-General
Code	Phrase
P101	If medical advice is needed,have product container or label at hand.
P102	Keep out of reach of children.
P103	Read label before use
Prevention
Code	Phrase
P201	Obtain special instructions before use.
P202	Do not handle until all safety precautions have been read and understood.
P210	Keep away from heat/sparks/open flames/hot surfaces. - No smoking.
P211	Do not spray on an open flame or other ignition source.
P220	Keep/Store away from clothing/combustible materials.
P221	Take any precaution to avoid mixing with combustibles
P222	Do not allow contact with air.
P223	Keep away from any possible contact with water, because of violent reaction and possible flash fire.
P230	Keep wetted
P231	Handle under inert gas.
P232	Protect from moisture.
P233	Keep container tightly closed.
P234	Keep only in original container.
P235	Keep cool
P240	Ground/bond container and receiving equipment.
P241	Use explosion-proof electrical/ventilating/lighting/equipment.
P242	Use only non-sparking tools.
P243	Take precautionary measures against static discharge.
P244	Keep reduction valves free from grease and oil.
P250	Do not subject to grinding/shock/friction.
P251	Pressurized container: Do not pierce or burn, even after use.
P260	Do not breathe dust/fume/gas/mist/vapours/spray.
P261	Avoid breathing dust/fume/gas/mist/vapours/spray.
P262	Do not get in eyes, on skin, or on clothing.
P263	Avoid contact during pregnancy/while nursing.
P264	Wash hands thoroughly after handling.
P265	Wash skin thouroughly after handling.
P270	Do not eat, drink or smoke when using this product.
P271	Use only outdoors or in a well-ventilated area.
P272	Contaminated work clothing should not be allowed out of the workplace.
P273	Avoid release to the environment.
P280	Wear protective gloves/protective clothing/eye protection/face protection.
P281	Use personal protective equipment as required.
P282	Wear cold insulating gloves/face shield/eye protection.
P283	Wear fire/flame resistant/retardant clothing.
P284	Wear respiratory protection.
P285	In case of inadequate ventilation wear respiratory protection.
P231 + P232	Handle under inert gas. Protect from moisture.
P235 + P410	Keep cool. Protect from sunlight.
Response
Code	Phrase
P301	IF SWALLOWED:
P304	IF INHALED:
P305	IF IN EYES:
P306	IF ON CLOTHING:
P307	IF exposed:
P308	IF exposed or concerned:
P309	IF exposed or if you feel unwell:
P310	Immediately call a POISON CENTER or doctor/physician.
P311	Call a POISON CENTER or doctor/physician.
P312	Call a POISON CENTER or doctor/physician if you feel unwell.
P313	Get medical advice/attention.
P314	Get medical advice/attention if you feel unwell.
P315	Get immediate medical advice/attention.
P320
P302 + P352	IF ON SKIN: wash with plenty of soap and water.
P321
P322
P330	Rinse mouth.
P331	Do NOT induce vomiting.
P332	IF SKIN irritation occurs:
P333	If skin irritation or rash occurs:
P334	Immerse in cool water/wrap n wet bandages.
P335	Brush off loose particles from skin.
P336	Thaw frosted parts with lukewarm water. Do not rub affected area.
P337	If eye irritation persists:
P338	Remove contact lenses, if present and easy to do. Continue rinsing.
P340	Remove victim to fresh air and keep at rest in a position comfortable for breathing.
P341	If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P342	If experiencing respiratory symptoms:
P350	Gently wash with plenty of soap and water.
P351	Rinse cautiously with water for several minutes.
P352	Wash with plenty of soap and water.
P353	Rinse skin with water/shower.
P360	Rinse immediately contaminated clothing and skin with plenty of water before removing clothes.
P361	Remove/Take off immediately all contaminated clothing.
P362	Take off contaminated clothing and wash before reuse.
P363	Wash contaminated clothing before reuse.
P370	In case of fire:
P371	In case of major fire and large quantities:
P372	Explosion risk in case of fire.
P373	DO NOT fight fire when fire reaches explosives.
P374	Fight fire with normal precautions from a reasonable distance.
P376	Stop leak if safe to do so. Oxidising gases (section 2.4) 1
P377	Leaking gas fire: Do not extinguish, unless leak can be stopped safely.
P378
P380	Evacuate area.
P381	Eliminate all ignition sources if safe to do so.
P390	Absorb spillage to prevent material damage.
P391	Collect spillage. Hazardous to the aquatic environment
P301 + P310	IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.
P301 + P312	IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.
P301 + P330 + P331	IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
P302 + P334	IF ON SKIN: Immerse in cool water/wrap in wet bandages.
P302 + P350	IF ON SKIN: Gently wash with plenty of soap and water.
P303 + P361 + P353	IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.
P304 + P312	IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell.
P304 + P340	IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.
P304 + P341	IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338	IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P306 + P360	IF ON CLOTHING: Rinse Immediately contaminated CLOTHING and SKIN with plenty of water before removing clothes.
P307 + P311	IF exposed: call a POISON CENTER or doctor/physician.
P308 + P313	IF exposed or concerned: Get medical advice/attention.
P309 + P311	IF exposed or if you feel unwell: call a POISON CENTER or doctor/physician.
P332 + P313	IF SKIN irritation occurs: Get medical advice/attention.
P333 + P313	IF SKIN irritation or rash occurs: Get medical advice/attention.
P335 + P334	Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages.
P337 + P313	IF eye irritation persists: Get medical advice/attention.
P342 + P311	IF experiencing respiratory symptoms: call a POISON CENTER or doctor/physician.
P370 + P376	In case of fire: Stop leak if safe to Do so.
P370 + P378	In case of fire:
P370 + P380	In case of fire: Evacuate area.
P370 + P380 + P375	In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion.
P371 + P380 + P375	In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion.
Storage
Code	Phrase
P401
P402	Store in a dry place.
P403	Store in a well-ventilated place.
P404	Store in a closed container.
P405	Store locked up.
P406	Store in corrosive resistant/ container with a resistant inner liner.
P407	Maintain air gap between stacks/pallets.
P410	Protect from sunlight.
P411
P412	Do not expose to temperatures exceeding 50 oC/ 122 oF.
P413
P420	Store away from other materials.
P422
P402 + P404	Store in a dry place. Store in a closed container.
P403 + P233	Store in a well-ventilated place. Keep container tightly closed.
P403 + P235	Store in a well-ventilated place. Keep cool.
P410 + P403	Protect from sunlight. Store in a well-ventilated place.
P410 + P412	Protect from sunlight. Do not expose to temperatures exceeding 50 oC/122oF.
P411 + P235	Keep cool.
Disposal
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P501	Dispose of contents/container to ...
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Physical hazards
Code	Phrase
H200	Unstable explosive
H201	Explosive; mass explosion hazard
H202	Explosive; severe projection hazard
H203	Explosive; fire, blast or projection hazard
H204	Fire or projection hazard
H205	May mass explode in fire
H220	Extremely flammable gas
H221	Flammable gas
H222	Extremely flammable aerosol
H223	Flammable aerosol
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H226	Flammable liquid and vapour
H227	Combustible liquid
H228	Flammable solid
H229	Pressurized container: may burst if heated
H230	May react explosively even in the absence of air
H231	May react explosively even in the absence of air at elevated pressure and/or temperature
H240	Heating may cause an explosion
H241	Heating may cause a fire or explosion
H242	Heating may cause a fire
H250	Catches fire spontaneously if exposed to air
H251	Self-heating; may catch fire
H252	Self-heating in large quantities; may catch fire
H260	In contact with water releases flammable gases which may ignite spontaneously
H261	In contact with water releases flammable gas
H270	May cause or intensify fire; oxidizer
H271	May cause fire or explosion; strong oxidizer
H272	May intensify fire; oxidizer
H280	Contains gas under pressure; may explode if heated
H281	Contains refrigerated gas; may cause cryogenic burns or injury
H290	May be corrosive to metals
Health hazards
Code	Phrase
H300	Fatal if swallowed
H301	Toxic if swallowed
H302	Harmful if swallowed
H303	May be harmful if swallowed
H304	May be fatal if swallowed and enters airways
H305	May be harmful if swallowed and enters airways
H310	Fatal in contact with skin
H311	Toxic in contact with skin
H312	Harmful in contact with skin
H313	May be harmful in contact with skin
H314	Causes severe skin burns and eye damage
H315	Causes skin irritation
H316	Causes mild skin irritation
H317	May cause an allergic skin reaction
H318	Causes serious eye damage
H319	Causes serious eye irritation
H320	Causes eye irritation
H330	Fatal if inhaled
H331	Toxic if inhaled
H332	Harmful if inhaled
H333	May be harmful if inhaled
H334	May cause allergy or asthma symptoms or breathing difficulties if inhaled
H335	May cause respiratory irritation
H336	May cause drowsiness or dizziness
H340	May cause genetic defects
H341	Suspected of causing genetic defects
H350	May cause cancer
H351	Suspected of causing cancer
H360	May damage fertility or the unborn child
H361	Suspected of damaging fertility or the unborn child
H361d	Suspected of damaging the unborn child
H362	May cause harm to breast-fed children
H370	Causes damage to organs
H371	May cause damage to organs
H372	Causes damage to organs through prolonged or repeated exposure
H373	May cause damage to organs through prolonged or repeated exposure
Environmental hazards
Code	Phrase
H400	Very toxic to aquatic life
H401	Toxic to aquatic life
H402	Harmful to aquatic life
H410	Very toxic to aquatic life with long-lasting effects
H411	Toxic to aquatic life with long-lasting effects
H412	Harmful to aquatic life with long-lasting effects
H413	May cause long-lasting harmful effects to aquatic life
H420	Harms public health and the environment by destroying ozone in the upper atmosphere

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[ CAS No. 65-46-3 ] {[proInfo.proName]}

Quality Control of [ 65-46-3 ]

Related Doc. of [ 65-46-3 ]

Product Details of [ 65-46-3 ]

Safety of [ 65-46-3 ]

Application In Synthesis of [ 65-46-3 ]

[ 65-46-3 ] Synthesis Path-Upstream 1~7

[ 65-46-3 ] Synthesis Path-Downstream 1~10

Similar Product of [ 65-46-3 ]

Precautionary Statements-General

Prevention

Response

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Physical hazards

Health hazards

Environmental hazards

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