Abstract: Fluorescent tracer dyes are popularly used in agricultural spray application studies to evaluate on-target canopy deposition and off-target drift, and the methods/instruments used for evalu ating dye deposit must assure reliability of the data generated. A laboratory setup was con figured to adapt a submersible fluorometer for controlled and reliable measurement of pyranine dye fluorescence in liquid samples. The system setup was blanked using a clean sam ple of de-ionized water and calibrated using standard dye solutions of known concentration (100 to 1,000,000 parts per trillion [ppt]) made from two dyes of different levels of purity (pyranine 98 % and pyranine 85 %). Pyranine 85 % fluorescence readings were consistently lower with reduction factor averaging ～0.62 times that of pyranine 98 % within a range from 500to1,000,000 ppt. The results were verified using a standard laboratory fluorometer. Spray deposition assessment of string, plastic card, cotton ribbon, and artificial foliage samplers was accomplished using the configured fluorometer system setup. The results showed no signifi cant difference among dye purity levels (P=0.430), no statistically significant interaction be tween dye purity and concentration (P=0.484), and no statistically significant interaction between dye purity and sampler type (P=0.173). Consequently, the configured setup can pro duce measurements with similar quality as the standard laboratory fluorometer and both dyes tested may be equally used for spray deposition and drift assessments, or for similar applications.
HPTS;Solvent Green 7;NSC 97285;Pyranine concentrated;Japan Green No. 204;Green No. 204;Green 204;D & C Green no. 8;8-Hydroxypyrene-1,3,6-trisulfonic Acid (sodium salt)
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In 2000 ml three-necked flask into 1200 grams of concentrated sulfuric acid, stirring into the 120 grams of flowers, a little warming to promoteAfter all the dissolved, slowly warming to 90 ° C for 1 hour, three bottles with a water bath to cool below 40 ° C, drop the liquid sulfur black 480 grams, 3 hours drop finished. And then to maintain the temperature of about 100 ° C reaction for 5 hours, down to room temperature, the three bottles of material into the 720 grams of ice water, into a sticky material, filter dry filter cake, get pyrene tetrasulfonic acid tetrasodium salt. And then the pyrene tetrasulfonic acid tetrasodium salt dissolved in 1000 ml of water into the 2000 ml three-necked flask, stirring with a plate of sodium hydroxide to neutral, then add 300 grams of flaky sodium hydroxide, slowly warming To micro-boiling state for 5 hours. After cooling, the first with hydrochloric acid and PH value of 7 or so, then add 100 grams of sodium chloride stirring dissolved, slowly precipitated light yellow solid, filtered and dried to get 8-hydroxy-1,3, 6-pyrene three Sodium sulfonate salt about 245 grams.
Reference：
[1] Patent: CN105272886, 2016, A, . Location in patent: Paragraph 0013; 0026; 0027
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[ 93253-93-1 ]
[ 6358-69-6 ]
Reference：
[1] Journal of the American Chemical Society, 1984, vol. 106, # 26, p. 8086 - 8093
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