Elucidating the Role of Optical Activity of Polymers in Protein-Polymer Interactions
Jahan, Samin
;
Doyle, Catherine
;
Ghimire, Anupama
, et al.
Polymers,2023,16(1):65.
DOI:
10.3390/polym16010065
PubMed ID:
38201730
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Abstract: Proteins are biomols. with potential applications in agriculture, food sciences, pharmaceutics, biotechnol., and drug delivery. Interactions of hydrophilic and biocompatible polymers with proteins may impart proteolytic stability, improving the therapeutic effects of biomols. and also acting as excipients for the prolonged storage of proteins under harsh conditions. The interactions of hydrophilic and stealth polymers such as poly(ethylene glycol), poly(trehalose), and zwitterionic polymers with various proteins are well studied. This study evaluates the mol. interactions of hydrophilic and optically active poly(vitamin B5 analogus methacrylamide) (poly(B5AMA)) with model proteins by fluorescence spectroscopy, NMR (NMR) spectroscopy, and CD (CD) spectroscopy anal. The optically active hydrophilic polymers prepared using chiral monomers of R-(+)- and S-(-)-B5AMA by the photo-iniferter reversible addition fragmentation chain transfer (RAFT) polymerization showed concentration-dependent weak interactions of the polymers with bovine serum albumin and lysozyme proteins. Poly(B5AMA) also exhibited a concentration-dependent protein stabilizing effect at elevated temperatures, and no effect of the stereoisomers of polymers on protein thermal stability was observed NMR anal., however, showed poly(B5AMA) stereoisomer-dependent changes in the secondary structure of proteins.
Keywords:
chiral materials ;
protein-polymer interactions ;
antifouling ;
protein stabilizing
Purchased from AmBeed:
5405-40-3
Photoiniferter RAFT Polymerization of Optically Active Hydrophilic Vitamin B5 Analogous Methacrylamide
Combita, Diego
;
Pamunuwegedara, Randima
;
Ahmed, Marya
ACS Appl. Polym. Mater.,2023,5(3):2289-2297.
DOI:
10.1021/acsapm.3c00168
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Abstract: Chiroptical polymers are interesting due to diverse applications in the field of chromatog., biomedicines, and catalysis. This study describes the synthesis of new types of optically active polymers of vitamin B5 analogous methacrylamide (B5AMA) using a photoiniferter polymerization approach at ambient temperatures Enantiomers of B5AMA monomer ((R) and (S)) were prepared by the ring opening method and were characterized for their optical activity. The monomers were then polymerized by a photoiniferter polymerization approach using two different thiocarbonylthio (TCT) compounds as iniferters. Both TCTs showed controlled polymerization of B5AMA enantiomers and yielded polymers of opposite optical rotation. The enantiomeric polymers of B5AMA exhibited a complementary mirror-image Cotton effect to each other in the CD (CD) spectra, and 13C NMR anal. revealed the formation of syndiotactic polymer structures. To the best of our knowledge, this is the first study that depicts the successful synthesis of optically active macromols. by a photoiniferter polymerization approach.
Keywords:
enantiomers ;
photoiniferter polymerization ;
polymer tacticity ;
optically active polymers ;
random coil structure ;
pendant group chirality
Purchased from AmBeed:
5405-40-3
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