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CAS No. : | 40246-10-4 | MDL No. : | MFCD00800711 |
Formula : | C22H22O10 | Boiling Point : | - |
Linear Structure Formula : | - | InChI Key : | OZBAVEKZGSOMOJ-MIUGBVLSSA-N |
M.W : | 446.40 | Pubchem ID : | 187808 |
Synonyms : |
Glycitein 7-O-β-glucoside;Glycitein-7-β-O-glucoside;HY-N0012;ZX-AFC000687;Glycitein-7-beta-O-glucoside
|
Chemical Name : | 3-(4-Hydroxyphenyl)-6-methoxy-7-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-4H-chromen-4-one |
Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P261-P305+P351+P338 | UN#: | N/A |
Hazard Statements: | H302-H315-H319 | Packing Group: | N/A |
GHS Pictogram: |
* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With acetic acid; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. | |
With acetic acid; In ethyl acetate; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. | |
With acetic acid; In 2,2,4-trimethylpentane; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. |
With acetic acid; In cyclohexane; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. | |
With acetic acid; In hexane; acetone; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. | |
With acetic acid; In hexane; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. | |
With acetic acid; In water; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. | |
With acetic acid; In Octanoic acid; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. | |
With acetic acid; In acetone; at 20℃; for 0.5h;Purification / work up; | 10.0 gram aliquots of"Solgen 40" (containing 26.9% genistin, 11.9% daidzin, 2.0% glycetin; negligible aglycon content; genistin-to-daidzin ratio 2.3 : 1) were charged to a 100 ml ERLENMEYER flask equipped with a magnetic stirring bar. Varying amounts of solvents comprising glacial acetic acid (GAA) and various co-solvents were added and the resulting slurry was stirred at ambient temperature for 30 minutes, then filtered on a Buechner funnel through Whatman No.541 paper. The filter cakes were washed with 10 milliliters of the solvent employed, dried in vacuo at 80C., and subjected to HPLC analysis. The results are tabulated below. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With hydrogenchloride; In methanol; water; at 66℃; for 0.25h;Heating / reflux;Purification / work up; | 50.0 grams OF ADM Novasoy (22.16% genistin + genistein; 18.56% daidzin + daidzein; 5.04% glycetin + glycetein; total aglycons 1.70% ; total isoflavone content 45.39% by wt.; genistin-to daidzin ratio 1.2 : 1) was added to 250 ml of methanol and 50 ml of concentrated hydrochloric acid in an ERLENMEYER flask equipped with a magnetic stirring bar and reflux condenser. The mixture was heated on a hotplate to reflux (66 C.), maintained at reflux for 15 minutes, then cooled to 15 C. The resulting slurry was filtered at this temperature on a Buechner funnel through Whatman No.541 filter paper. Filtration was very rapid, with no blinding. The off-white filter cake solids were washed with a modest volume of 5: 1 methanol: HCL and dried in a vacuum oven at 80 C. to give 10.13 grams of off-white solids containing: 55.4% by wt. genistin (5.61 grams, or 50.7% of that present in the starting material) 15.65% by wt. daidzin (1.58 grams, or 17.1% of that present in the starting material) 0.86% by wt. glycetin (0.087 grams, or 3.4% of that present in the starting material) 72.00% total isoflavones; genistin-to-daidzin ratio = 3.54 : 1 No isoflavone aglycons were detected. |