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With dicyclohexyl-carbodiimide; In N,N-dimethyl-formamide; at 20℃; for 25h;Molecular sieve; Cooling with ice;
Mono(6-deoxy-6-amino)-beta-CD (amino-beta-CD) was obtained in three steps from the parent beta-CD and purified by Sephadex chromatography with aqueous solution (Belanger & Perly, 1992; Croft & Bartsch, 1983). A solution of amino-beta-CD (2.2 g, 2 mmol), scutellarin (SCU, 1.1 g, 2.4 mmol), DCC (0.56 g, 3 mmol) and a small amount of 4 A molecular sieves in DMF (50 mL) were stirred for 1 h in an ice bath and subsequently for 24 h at room temperature.The insoluble materials were removed by filtration. The filtrate was poured into 300 mL of acetone. The precipitate was collected and subsequently purified on a Sephadex G-25 column with water as eluent. The residue was dried in vacuo, and amino-beta-CD conjugate was obtained in yield of 46percent.
General procedure: The CDs were prepared using a reported method[32] the required CD (0.01 mM) and SCU (0.03 mM, 13.9 mg) were added to ultra pure water (10 mL), and the mixture was stirred for 4 days at room temperature in the dark. Then, the precipitate was removed by filtration, and the filtrate was evaporated under reduced pressure to remove the solvent and dried under vacuum to obtain the SCU/CDs complexes.
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