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Chemical Structure| 200626-61-5 Chemical Structure| 200626-61-5

Structure of ITSA-1
CAS No.: 200626-61-5

Chemical Structure| 200626-61-5

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CAS No.: 200626-61-5

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ITSA-1 is a histone deacetylase (HDAC) activator that counteracts trichostatin A (TSA)-induced cell cycle arrest, histone acetylation, and transcriptional activation.

Synonyms: ITSA-1

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Product Details of ITSA-1

CAS No. :200626-61-5
Formula : C13H7Cl2N3O
M.W : 292.12
SMILES Code : O=C(N1N=NC2=CC=CC=C21)C3=CC=C(Cl)C=C3Cl
Synonyms :
ITSA-1
InChI Key :UVNLAUGZMOPBPR-UHFFFAOYSA-N
Pubchem ID :771910

Safety of ITSA-1

GHS Pictogram:
Signal Word:Warning
Hazard Statements:H302
Precautionary Statements:P280-P305+P351+P338

Related Pathways of ITSA-1

epigenetics

Isoform Comparison

Biological Activity

In Vitro:

Cell Line
Concentration Treated Time Description References
Human Umbilical Vein Endothelial Cells (HUVECs) 150 μM 12 h ITSA-1 increased the expressions of H3K9me3, pHDAC3, HDAC3, and p-HDAC4/5/7 in ALS-treated HSVECs and reduced VCAM-1 expression, indicating that ITSA-1 could reverse ALS-induced vEC inflammation. PMC8063156
Human Saphenous Vein Endothelial Cells (HSVECs) 150 μM 12 h ITSA-1 increased the expressions of H3K9me3, pHDAC3, HDAC3, and p-HDAC4/5/7 in ALS-treated HSVECs and reduced VCAM-1 expression, indicating that ITSA-1 could reverse ALS-induced vEC inflammation. PMC8063156
growth plate chondrocytes 50 nM, 100 nM, 150 nM 24 hours To observe the effect of ITSA-1 on the morphology and expression of growth and differentiation marker proteins in chondrocytes, it was found that chondrocytes treated with ITSA-1 were oblate or oval in shape, with different cell sizes and a loose and disordered arrangement, indicating that the chondrocytes were still in the proliferative stage. PMC10342131
BMMSCs 12.5 μM 3 weeks ITSA-1 treatment restored osteoblast differentiation and mineralization in CBS+/? BMMSCs PMC6189133
CNE2 cells 10 μM 48 hours ITSA-1 reversed the TSA-impeded proliferation, migration, and HDACs expression in CNE2 cells and re-induced the cells to turn into epithelial cell phenotypes. PMC6431036
C666-1 cells 10 μM 48 hours ITSA-1 reversed the TSA-impeded proliferation, migration, and HDACs expression in C666-1 cells and re-induced the cells to turn into epithelial cell phenotypes. PMC6431036

In Vivo:

Species
Animal Model
Administration Dosage Frequency Description References
C57BL/6 J CBS+/? mice Intraperitoneal injection 0.5 mg/kg body weight 3 times/week for 8 weeks ITSA-1 treatment restored HDAC3 activity in CBS+/? mice, reduced inflammatory signaling, and improved bone formation PMC6189133

Protocol

Bio Calculators
Preparing Stock Solutions 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.42mL

0.68mL

0.34mL

17.12mL

3.42mL

1.71mL

34.23mL

6.85mL

3.42mL

Dissolving Methods
Please choose the appropriate dissolution scheme according to your animal administration guide.For the following dissolution schemes, clear stock solution should be prepared according to in vitro experiments, and then cosolvent should be added in turn:

in order to ensure the reliability of the experimental results, the clarified stock solution can be properly preserved according to the storage conditions; The working fluid for in vivo experiment is recommended to be prepared now and used on the same day;

The percentage shown in front of the following solvent refers to the volume ratio of the solvent in the final solution; If precipitation or precipitation occurs in the preparation process, it can be assisted by heating and/or ultrasound.
Protocol 1
Protocol 2

References

 

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