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[ CAS No. 188627-80-7 ] {[proInfo.proName]}

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Chemical Structure| 188627-80-7
Chemical Structure| 188627-80-7
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Herr, Kevin ; Fleckenstein, Max ; Brodrecht, Martin , et al. DOI: PubMed ID:

Abstract: A novel specific spin-labeling strategy for bioactive mols. is presented for eptifibatide (integrilin) an antiplatelet aggregation inhibitor, which derives from the venom of certain rattlesnakes. By specifically labeling the disulfide bridge this mol. becomes accessible for anal. techniques such as ESR (EPR) and solid state Dynamic Nuclear Polarization (DNP). The necessary spin-label was synthesized and inserted into the disulfide bridge of eptifibatide via reductive followed by insertion by a double Michael addition under physiol. conditions. This procedure is universally applicable for disulfide containing biomols. and is expected to preserve their tertiary structure with minimal change due to the small size of the label and restoring of the previous disulfide connection. HPLC and MS anal. show the successful introduction of the spin label and EPR spectroscopy confirms its activity. DNP-enhanced solid state NMR experiments show signal enhancement factors of up to 19 in 13C CP MAS experiments which corresponds to time saving factors of up to 361. This clearly shows the high potential of our new spin labeling strategy for the introduction of site selective radical spin labels into biomols. and biosolids without compromising its conformational integrity for structural investigations employing solid-state DNP or advanced EPR techniques.

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Product Details of [ 188627-80-7 ]

CAS No. :188627-80-7 MDL No. :MFCD05662245
Formula : C35H49N11O9S2 Boiling Point : -
Linear Structure Formula :- InChI Key :CZKPOZZJODAYPZ-LROMGURASA-N
M.W : 831.96 Pubchem ID :448812
Synonyms :
Chemical Name :2-((3R,11S,17S,20S,25aS)-20-((1H-Indol-3-yl)methyl)-3-carbamoyl-11-(4-guanidinobutyl)-1,9,12,15,18,21-hexaoxodocosahydro-1H-pyrrolo[2,1-g][1,2,5,8,11,14,17,20]dithiahexaazacyclotricosin-17-yl)acetic acid

Safety of [ 188627-80-7 ]

Signal Word:Warning Class:N/A
Precautionary Statements:P260-P314-P501 UN#:N/A
Hazard Statements:H373 Packing Group:N/A
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