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CAS No. : | 116313-85-0 | MDL No. : | MFCD00871897 |
Formula : | C7H5NO5 | Boiling Point : | - |
Linear Structure Formula : | C6H2CHONO2OHOH | InChI Key : | BBFJODMCHICIAA-UHFFFAOYSA-N |
M.W : | 183.12 | Pubchem ID : | 5748957 |
Synonyms : |
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Signal Word: | Warning | Class: | N/A |
Precautionary Statements: | P280-P305+P351+P338-P310 | UN#: | N/A |
Hazard Statements: | H302-H315-H319-H332-H335 | Packing Group: | N/A |
GHS Pictogram: |
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* All experimental methods are cited from the reference, please refer to the original source for details. We do not guarantee the accuracy of the content in the reference.
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
With xanthin; for 20.0h;pH 7.4;Enzymatic reaction; | Reaction of XO and DHNB [0084] To determine how DHNB inhibits XO enzyme activity, 30 muM DHNB was incubated with 15 mU/ml (or 30 nM) XO in phosphate buffer (pH 7.4) and xanthine was then added to initiate the reaction as discussed above. The inhibition of DHNB on XO activity lasted up to 20 h. After that, the enzymatic activity of XO was recovered. The optical spectral change of DHNB was measured in a system without xanthine, i.e., 30 muM DHNB with 15 mU/ml (or 30 nM) XO in phosphate buffer. The absorption of DHNB at 327 nm decreased with time and a new peak appeared at 270 nm (see FIG. 6A). The decay rate was in the range of 10-10 mol/L/s and was pH dependent, i.e., the higher the pH value, the faster DHNB decayed (see FIG. 6B). Without XO, however, DHNB itself was very stable. At room temperature, DHNB was converted by XO enzyme to a product which has no inhibitory effect on XO and thus recovered the XO activity as the concentration of DHNB decreased. The UV-VIS spectrum of the product was different from that of DHNB. HPLC analysis of DHNB/XO showed a new peak which was more polar than DHNB (see FIG. 6C). Mass spectrometric analysis of the product gave a molecular ion (EM-HI) peak at m/z 198 in the EI mass spectrum, while DHNB showed EM-Fir peak at m/z 182 (see FIG. 6D, E). The MS/MS of m/z 182 of DHNB gave several typical fragments such as m/z 165 ([M-H-OH]-), 152 ([M-H-CHO-H]-) and 135 (m/z 152-OH). However, the MS/MS of molecular ion at m/z 198 of the product gave a first main fragment at m/z 154, a mass difference of 44 indicating a loss of CO2, which further loses a -OH to give a fragment at m/z 137. Based on the mass spectrum, the product is 3,4-dihydroxy-5-nitrobenzoic acid, implying that DHNB is oxidized to the acid by the enzyme. |
Yield | Reaction Conditions | Operation in experiment |
---|---|---|
90.6% | With hydrogenchloride; In methanol; water;Reflux; | General procedure: 3,4-Dihydroxy-5-nitrobenzaldehyde (14, 1.092 mmol) and theappropriate 1-tetralone (15), 1-indanone (16), 3-coumaranone (17) oracetophenone (21) reagents, or 4-chromanone (18) or 4-thiochromanone(19) (1.092 mmol) were dissolved in a mixture of 6 mL HCl(32%) and 4 mL methanol, and heated under reflux for 24-26 h. Theprogress of the reactions was monitored with silica gel TLC with amixture of petroleum ether and ethyl acetate (1:1) as mobile phase.Upon completion, the reaction was quenched with addition ice water(50 mL), upon which a precipitate formed. The precipitated materialwas collected by filtration and dried at 60 C for 48 h before furtheranalysis. Compound 5b and 20c were purified by recrystallisation fromethanol and acetonitrile, respectively. |
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