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[ CAS No. 10417-94-4 ] {[proInfo.proName]}

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Chemical Structure| 10417-94-4
Chemical Structure| 10417-94-4
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Lee, Jin ; Park, Hyun-Ah ; Shin, Kyung-Chul , et al. DOI: PubMed ID:

Abstract: Resolvin D5 (RvD5), 7S,17S-dihydroxy-4Z,8E,10Z,13Z,15E,19Z-docosahexaenoic acid (DHA) is a specialized pro-resolving mediator (SPM) generated in human macrophages. It is implicated in the resolution of inflammation and synthesized using an inefficient chem. process. Here, DHA-enriched oil hydrolyzate was prepared from oils by lipase with resin treatment and solvent extraction The reaction factors on the biotransformation of oil hydrolyzate into RvD5 were optimized using Escherichia coli expressing arachidonate double-oxygenating 15S-lipoxygenase. After optimization, the cells converted 5.0 mM (1.64 g/L) DHA in oil hydrolyzate into 4.0 mM (1.44 g/L) RvD5 in a bioreactor for 3.0 h, which was 15-fold higher than that in a flask before optimization, and RvD5 with a purity of > 97% was prepared from reaction solution by treatments of resins. This is the first trial for the production of C22-dihydroxy fatty acid using a bioreactor. This study will contribute to the large-scale production of SPMs from oils.

Keywords: Lipoxygenase ; Resolvin D5 ; Docosahexaenoic acid-enriched oil hydrolysate ; Biotransformation ; Bioreactor

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Lee, Hyejin ; Yoon, Hye-Ran ; DOI:

Abstract: We developed and validated a fast, sensitive, and accurate anal. method using ultra high-pressure liquid chromatog.-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) for simultaneous extraction and quantification of 5 targeted omega fatty acids on human dried serum spots. MS/MS was performed with multiple reaction monitoring (MRM) mode under neg. electrospray ionization. We targeted omega fatty acids which are likely to have a pos. effect on various inflammatory reactions such as asthma, chronic obstructive pulmonary diseases and rheumatoid arthritis. If applicable, it is necessary to determine them as asthma biomarkers for intervention and public health. The coefficient of determination (r2) of calibration curve exhibited a good linear relationship between 0.9942 and 0.9963 in a range of 0.05-5μg mL-1. The anal. method was validated with excellent sensitivity (limit of detection; 0.005-0.01μg mL-1, limit of quantification; 0.03-0.1μg mL) that allows targeted analyses of omega fatty acids. We obtained the recoveries of 92.6 to 110.4% (RSD, 0.2-9.6%) for intraday and 92.6 to 107.6% (RSD, 1.4-7.0%) for interday. Clin. correlation between the healthy subjects and asthmatic patients sera was evaluated with the ratio of omega-3/omega-6 fatty acids. The developed method combines the advantages of easy handling dried filter paper and UHPLC-MS/MS for effectiveness of extraction and analyses step. We evaluated the validation for the quantification and applicability of human serum as a possible role of asthma biomarkers.

Keywords: Omega fatty acids ; Dried human serum spot ; UHPLC-MS ; MS ; Negative ionization ; Asthma

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Product Details of [ 10417-94-4 ]

CAS No. :10417-94-4 MDL No. :MFCD00065716
Formula : C20H30O2 Boiling Point : -
Linear Structure Formula :- InChI Key :JAZBEHYOTPTENJ-JLNKQSITSA-N
M.W : 302.45 Pubchem ID :446284
Synonyms :
EPA;Timnodonic acid;Icosapent, Eicosapentaenoic acid, Timnodonic acid, Vascepa, Epadel, EPAX;omega-3 Fatty acid;5-LOX inhibitor];C20:5 (all cis-5,8,11,14,17) Fatty acid [Anti-hyperlipoproteinenic agent;Eicosapentaenoic acid (all cis-5,8,11,14,17)
Chemical Name :cis-5,8,11,14,17-Eicosapentaenoic acid

Safety of [ 10417-94-4 ]

Signal Word:Danger Class:8
Precautionary Statements:P210-P264-P271-P280-P305+P351+P338-P301+P330+P331-P303+P361+P353-P304+P340-P310-P370+P378-P363-P403+P233-P501 UN#:3265
Hazard Statements:H314-H227 Packing Group:Ⅲ
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