Product Details
| Product Name:
UAMC-3203 |
CAS No.:
2271358-64-4 |
| Purity:
98.81% |
Supply Ability:
10g |
| Release date:
2024/11/13 |
Product Introduction
Bioactivity
| 名稱 | UAMC-3203 |
| 描述 | UAMC-3203 is a potent and selective ferroptosis inhibitor with an IC50 value of 12 nM. |
| 細胞實驗 | In order to determine IC50-values, human neuroblastoma cells (IMR-32) were seeded in a 96-well plate at a density of 25,000 cells/well. The next day, the cells were pretreated for 1h (in triplicates) with a 1/3 dilution series of ferrostatin-1 analogues ranging from 5μM to 0.68nM and Sytox Green (1.6 μM) and a 1/2 dilution series of ferrostatin-1 analogues ranging from 200 nM to 0.78nM and Sytox Green (1.6 μM) for erastin and ferrous ammonium sulphate respectively. After stimulating the cells with erastin (10 μM) or ferrous ammonium sulphate (600 μM), the plate was transferred to a temperature- and CO2-controled FLUOstar Omega fluorescence plate reader. Sytox Green intensity was measured after 13h using an excitation filter of 485 nm and an emission filter of 520 nm. In each setup, Triton-X100 (0.05%) was used to induce lyses of the cells in 6 wells/plate, and was used as 100% cell death reference. The percentage of the cell death was calculated by the formula ((AVG[erastin] - AVG[background]) /(AVG[Triton-X100] - AVG[background])) × 100. Cell death percentage was plotted in GraphPad Prism 6, and IC50-values were calculated using a sigmoidal dose-response (variable slope) curve. |
| 動物實驗 | Vehicle solution (2% DMSO) or compound was administered daily at a concentration of 2 mM (in 0.9% NaCl containing 2% DMSO; 200 μL / 20 g body weight) by intraperitoneal injection. Body temperature and weight were monitored daily. On day 28 the mice were anesthetized with isoflurane and blood was sampled. Hereafter, mice were sacrificed by cervical dislocation. ALT, AST and LDH levels in plasma were measured. CK, creatinine, urea and troponin T were determined. |
| 體外活性 | UAMC-3203 exhibits even lower IC50-values (10 nM in IMR32 Neuroblastoma cells.) combined with an excellent kinetic solubility (> 200 μM). |
| 體內(nèi)活性 | In blood plasma of mice having received a daily injection of compound UAMC-3203 over a period of 4 weeks, Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), LDH, creatine kinase (CK), creatinine, urea and troponin T levels were all comparable to background values detected in control mice. |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 50 mg/mL (106.01 mM)
|
| 關鍵字 | inhibit | Ferroptosis | Inhibitor | UAMC-3203 |
| 相關產(chǎn)品 | TBHQ | Acetylcysteine | Butylated hydroxytoluene | α-Vitamin E | Sorafenib | L-Glutamic acid | L-Glutathione reduced | Curcumin | Artemisinin | L-Cystine | L-Glutamic acid monosodium salt | Coenzyme Q10 |
| 相關庫 | 鐵死亡化合物庫 | 細胞凋亡化合物庫 | 經(jīng)典已知活性庫 | 抑制劑庫 | NO PAINS 化合物庫 | 已知活性化合物庫 | 細胞周期化合物庫 | 抗COVID-19化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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Recommended supplier
| Product name |
Price |
|
Suppliers |
Update time |
|
|
$/ |
|
Anhui Ruihan Technology Co., Ltd
|
2023-08-21 |
United States