Description of PyBOP from Watson Biotechnology Co., Ltd

Specifications(COA) of PyBOP CAS 128625-52-5 Coupling Reagent

**PyBOP (Benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate)** is a coupling reagent widely used in organic chemistry, particularly in **peptide synthesis**. It facilitates the formation of **amide bonds** by activating carboxyl groups to form amides from amines and carboxylic acids. Here's a more detailed overview of its applications:

### Key Applications:

1. **Peptide Bond Formation**:

   - PyBOP is primarily used for **coupling amino acids** during the synthesis of peptides. It converts the carboxyl group of one amino acid into a highly reactive intermediate, allowing it to couple with the amine group of another amino acid to form a peptide bond.

2. **Milder Reaction Conditions**:

   - Compared to other coupling reagents like DCC (Dicyclohexylcarbodiimide), PyBOP works under **milder conditions** and tends to produce fewer side reactions, such as racemization (which is undesirable in peptide synthesis).

3. **Improved Solubility**:

   - PyBOP is soluble in a variety of organic solvents, which enhances its use in solution-phase peptide synthesis.

4. **Less Formation of By-products**:

   - It generates fewer by-products compared to other coupling agents like DCC, which produces urea as a by-product.

### Mechanism:

In peptide synthesis, PyBOP forms an active **Oxyphosphonium intermediate** by reacting with the carboxylic acid group of the amino acid. This intermediate can then react with an amine to form the desired amide bond (peptide bond).

In summary, PyBOP is essential in organic and peptide chemistry for efficient, high-yield peptide bond formation with minimal side reactions.

Founded in 2006 and headquartered in Wuhan, Hubei, China, Watson Biotechnology Co., Ltd is a leading manufacturing powerhouse specializing in the production of Active Pharmaceutical Ingredients (APIs) and Intermediates, Food and Feed Additives, Agricultural and Aquaculture chemicals, and Fine Chemicals. As a dedicated factory, our operations are driven by a commitment to excellence and innovation, ensuring that our products meet the highest standards of quality and compliance.

With a robust team of over 100 skilled professionals, Watson Biotechnology has established itself as a trusted name in the industry. Our products are meticulously crafted to align with international standards such as USP (United States Pharmacopeia), EP (European Pharmacopoeia), and BP (British Pharmacopoeia), guaranteeing reliability and efficacy.

At Watson Biotechnology, we pride ourselves on our cutting-edge facilities and state-of-the-art technology, which enable us to deliver superior products that cater to a diverse range of needs across various sectors. Our dedication to continuous improvement and customer satisfaction drives us to maintain stringent quality control processes and foster an environment of innovation and growth.

Watson Biotechnology Co., Ltd is a professional manufacturer which provide PyBOP with good quality and best price, welcome to contact us to get COA/TDS/MSDS of PyBOP.