NADPH Tetrasodium salt

Empirical Formula (Hill Notation): C21H26N7Na4O17P3 · xH2O

Synonym(s): β-NADPH, Coenzyme II reduced tetrasodium salt, 2′-NADPH hydrate, β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, Triphosphopyridine nucleotide reduced tetrasodium salt, NADPH Na4, TPNH2 Na4, Dihydronicotinamide adenine dinucleotide phosphate tetrasodium salt

CAS Number: 2646-71-1

Molecular Weight: 833.35 (anhydrous basis)

Assay ≥97% (dry weight)

Form powder

Packaging pkg of 1 g 

pkg of 100 mg

pkg of 500 mg

λmax 260 and 340 nm

Shipped in  ice bag

Storage temp. 2-8°C

DESCRIPTION

General description

Amorphous powder bottled under nitrogen.

Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm

Absorbance maxima: 260 nm and 340 nm

Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4

pH value in water: 8.0 ± 1.0 (10 mg/ml)

Application

NADPH-RO has been used for the determination of thioredoxin reductase activity.

Biochem/physiol Actions

NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase.

Quality

Purity: =97% ?-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm)

Organic solvents: =3% (GC)

Preparation Note

Storage conditions (working solution): 2 to 8 °C

10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C.

Analysis Note

Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm

Other Notes

Packaged based on solid weight.