Product Details
| Product Name:
ML385 |
CAS No.:
846557-71-9 |
| Purity:
99.85% |
Supply Ability:
10g |
| Release date:
2024/11/16 |
Product Introduction
Bioactivity
| 名稱 | ML385 |
| 描述 | ML385 is an NRF2 inhibitor (IC50=1.9 μM) with novelty and specificity. ML385 has anti-inflammatory activity by modulating anti-oxidative stress through the inhibition of NRF2. ML385 also exhibits anti-tumor activity. |
| 細(xì)胞實(shí)驗(yàn) | cells are treated with ML385 for 36 h. An equal amount of CellTiter-Blue reagent is added to the wells and the fluorescence is measured after 30 min. The CellTiter-Blue reagent is discarded and the Caspase-Glo (100 μL) reagent is added to the cells and incubated at 37°C for an additional 60-90 min. The resulting luminescence is recorded and the caspase activity is normalized to cell number |
| 動物實(shí)驗(yàn) | Mice tumor xenografts are administered intraperitoneally ML385 (30 mg/kg). |
| 體外活性 | METHODS: Human lung cancer cells A549 were treated with ML385 (0.25-5 μM) for 12-72 h. The expression levels of target genes were detected by RT-qPCR.
RESULTS: ML385 dose-dependently and time-dependently decreased the transcriptional activity of NRF2. [1]
METHODS: Human lung cancer cells EBC1 were treated with ML385 (1-25 μM) for 48 h, and the expression levels of target proteins were detected by Western Blot.
RESULTS: NRF2 expression was inhibited by treatment with 5 μM ML385. When the concentration of ML385 was increased above 5 μM, the NRF2 protein level was restored. [2] |
| 體內(nèi)活性 | METHODS: To detect anti-tumor activity in vivo, ML385 (30 mg/kg) and carboplatin (5 mg/kg) were intraperitoneally injected into athymic nude mice harboring human lung cancer tumors A549 or H460 five times a week for three weeks.
RESULTS: Treatment with ML385 in combination with carboplatin showed a significant reduction in tumor growth. Although treatment with a single agent resulted in a reduction in tumor growth, the magnitude of these effects was variable between cell lines and did not reach statistical significance. [1]
METHODS: To investigate whether Nrf2 modulates acute liver failure (ACLF) through iron death, ML385 (30 mg/kg) was injected intraperitoneally four times per week for four weeks into BALB/c mice constructed in the ACLF model.
RESULTS: More severe histopathological lesions were observed in the ML385 group compared to the ACLF group. lipid peroxidation and liver injury were exacerbated by the Nrf2 inhibitor, ML385. [3] |
| 存儲條件 | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 50 mg/mL (97.73 mM)
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 3 mg/mL (5.86 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
|
| 關(guān)鍵字 | Ferroptosis | Inhibitor | inhibit | ML 385 | ML-385 | ML385 | Keap1-Nrf2 |
| 相關(guān)產(chǎn)品 | TBHQ | Acetylcysteine | Butylated hydroxytoluene | α-Vitamin E | Sorafenib | L-Glutathione reduced | Resveratrol | Curcumin | Artemisinin | L-Cystine | L-Glutamic acid monosodium salt | Coenzyme Q10 |
| 相關(guān)庫 | 非甾體類抗炎化合物庫 | 細(xì)胞凋亡化合物庫 | 鐵死亡化合物庫 | 神經(jīng)退行性疾病化合物庫 | 經(jīng)典已知活性庫 | 抑制劑庫 | PPI抑制劑庫 | 抗衰老化合物庫 | 已知活性化合物庫 | 細(xì)胞周期化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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