IBMX NEW
| Price | $33 | $47 | $58 |
| Package | 25mg | 50mg | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-18 |
Product Details
| Product Name: IBMX | CAS No.: 28822-58-4 |
| Purity: 99.86% | Supply Ability: 10g |
| Release date: 2024/11/18 |
Product Introduction
Bioactivity
| Name | IBMX |
| Description | IBMX (Methylisobutylxanthine) is a broad-spectrum phosphodiesterase (PDE) inhibitor with inhibitory activity against PDE3, PDE4, and PDE5 (IC50=6.5/26.3/31.7 μM). IBMX enhances the intracellular cAMP level. |
| Cell Research | Intracellular cyclic GMP and cyclic AMP concentrations in guinea-pig TSMCs were assayed as previously described. In brief, cells were grown in 24-well plates 10^5 cells per well. At confluence, monolayer cells were washed with phosphate buffer solution (PBS) and then incubated with KMUP-1 (0.1–100 μM) in the presence of 100 μM IBMX for 20 min. Incubation was terminated by the addition of 10% trichloroacetic acid (TCA). Cell suspensions were sonicated and then centrifuged at 2500 × g for 15 min at 4°C. To remove TCA, the supernatants were extracted three times with 5 volumes of water-saturated diethyl ether. Then, the supernatants were lyophilized and the cyclic GMP or AMP of each sample was determined by using commercially available radioimmunoassay kits [1]. |
| Animal Research | Male mice (25-35 g), obtained from the animal house of Faculty of Medicine, were kept in controlled environmental conditions (temperature: 23±2 oC; light-dark cycle: 7 a.m. to 7 p.m.) and were divided randomly into groups of seven. All test compounds were dissolved in DMSO and diluted to desired concentration with less than 1% DMSO. For the experiment, the test compound (IBMX, milrinone, MCPIP, mc1, mc2, mc5 or mc6) or solvent (control) was injected subcutaneously to mice at 1 mg/kg dosage twice a day (8:00 a.m. and 8:00 p.m.) for 7 days. On day 8, animals were anesthetized with intraperitoneal injection of thiopental (80 mg/kg) and blood samples were obtained from their hearts and then the liver was dissected. Each sample was centrifuged for 5 min and its serum was separated [3]. |
| In vitro | METHODS: CCDs isolated from HK-fed rats were pretreated with IBMX (100 μM) for 20 min and examined the effect of ANG II or cGMP on channel activity. RESULTS: IBMX activated ROMK channels and prevented further channel activation by ANG II. [1] METHODS: Primary cultures of guinea pig TSMC were assayed for the effect of KMUP-1 on cAMP and cGMP levels in the presence of IBMX (100 μM). RESULTS: IBMX and KMUP-1 significantly increased cAMP and cGMP levels. The effect of KMUP-1 alone on cAMP and cGMP levels was not significantly different from that in the presence of IBMX. [2] METHODS: Mammalian cell CHO was treated with IBMX (10-1000 μM) and whole cell currents were measured using the membrane clamp technique. RESULTS: A steady-state dose-response curve for the effect of IBMX on THIK-1 currents could be fitted with a Hill coefficient of 1 and an IC50 of 120 μM.[3] |
| In vivo | METHODS: To test the metabolic effects on mice, IBMX (1 mg/kg) was injected subcutaneously into mice twice daily for seven days. RESULTS: IBMX significantly increased blood glucose levels in mice (blood glucose, mg/dl, control=141, IBMX=210). [4] METHODS: To test the metabolic effects on hyperglycemic mice, glucose (0.5 g/kg) and IBMX (1 mg/kg) were injected i.v. in femoral veins into Wistar rats. RESULTS: In hyperglycemic rats, IBMX lowered blood glucose, IBMX did not change plasma insulin levels, and IBMX decreased hepatic glycogen stores. [4] |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | H2O : 2.22 mg/mL (10 mM), Sonication is recommended. DMSO : 16.7 mg/mL (75 mM) 10% DMSO+90% Saline : 1.67 mg/mL (7.51 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. Ethanol : 22.24 mg/mL (100 mM), Sonication is recommended. |
| Keywords | Phosphodiesterase (PDE) | IBMX | inhibit | Inhibitor |
| Inhibitors Related | Theophylline monohydrate | Roflumilast | Theobromine | Apremilast | Isoprenaline hydrochloride | Indomethacin | Icariin | Theophylline | Vardenafil hydrochloride | Doxofylline |
| Related Compound Libraries | 神經(jīng)退行性疾病化合物庫 | 經(jīng)典已知活性庫 | 抗阿爾茨海默癥化合物庫 | 抑制劑庫 | NO PAINS 化合物庫 | 代謝化合物庫 | 已知活性化合物庫 | 抗代謝疾病化合物庫 | 人代謝物化合物庫 | 核苷類化合物庫 |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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