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Common Name	Guanidine hydrochloride
CAS Number	50-01-1	Molecular Weight	95.531
Density	1.18 g/mL at 25 °C(lit.)	Boiling Point	132.9oC at 760 mmHg
Molecular Formula	CH6ClN3	Melting Point	180-185 °C(lit.)
MSDS	ChineseUSA	Flash Point	N/A
Symbol	GHS07	Signal Word	Warning

Use of Guanidine hydrochloride

Guanidine HCl, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.Target: OthersGuanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins. Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (> 1 M) causes co-operative unfolding of the molten globule state [1]. Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI+] and other prions of yeast. 5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase [2].

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