Description of Fluorescein isothiocyanate isomer I from Wuhan Fortuna Chemical Co.,Ltd

Specifications(COA) of High Quality Fluorescein isothiocyanate isomer I / FITC Powder CAS 3326-32-7 For Biochemical Studies / Scientific Research

Introduction of Fluorescein isothiocyanate isomer I.

Fluorescein isothiocyanate (FITC) isomer I is a fluorescent dye commonly used in various biological and biochemical applications. FITC is a derivative of fluorescein, a synthetic organic compound that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The isothiocyanate group in FITC allows it to covalently bind to amine groups on proteins, antibodies, and other biomolecules, making it a versatile tool for labeling and detection.

Key Applications of FITC Isomer I:

Flow Cytometry:FITC is widely used in flow cytometry to label antibodies that bind to specific cell surface markers. This allows for the identification and quantification of different cell populations based on their fluorescence characteristics.

Fluorescence Microscopy:FITC-labeled antibodies or other probes can be used to visualize the localization of specific proteins or other molecules within cells and tissues under a fluorescence microscope.

Immunofluorescence:In immunofluorescence assays, FITC-conjugated antibodies are used to detect the presence and distribution of antigens in biological samples. This technique is commonly used in research and diagnostic laboratories.

Western Blotting:FITC can be used to label secondary antibodies in Western blotting, allowing for the detection of specific proteins on a membrane using fluorescence imaging systems.

ELISA (Enzyme-Linked Immunosorbent Assay):FITC-labeled antibodies can be used in ELISA to detect the presence of specific antigens in a sample, with the fluorescence signal providing a quantitative readout.

Live Cell Imaging:FITC can be used to label live cells for various imaging applications, including tracking cell movement, proliferation, and interactions in real-time.

Properties of FITC Isomer I:

Excitation and Emission: FITC has an excitation peak around 495 nm and an emission peak around 519 nm, which corresponds to its bright green fluorescence.

Reactivity: The isothiocyanate group (-N=C=S) in FITC reacts with primary amines (-NH2) on proteins and other biomolecules, forming a stable thiourea bond.

Solubility: FITC is generally soluble in organic solvents like dimethyl sulfoxide (DMSO) and dimethylformamide (DMF), and it can also be dissolved in aqueous buffers for biological applications.

Handling and Storage:

Light Sensitivity: FITC is light-sensitive and should be protected from prolonged exposure to light to prevent photobleaching.

Storage: FITC and its conjugates should be stored at low temperatures (e.g., -20°C) and in the dark to maintain stability and fluorescence intensity.

Overall, FITC isomer I is a powerful and widely used tool in molecular biology, immunology, and cell biology for the fluorescent labeling and detection of various biomolecules.

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Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide Fluorescein isothiocyanate isomer I with good quality and best price, welcome to contact us to get COA/TDS/MSDS of Fluorescein isothiocyanate isomer I.